Challenges and prospects for developing genetic resistance in common wheat against stem rust in Western Siberia

Kelbin, Vasiliy N.; Сколотнева, Е. С.; Салина, Е. А.

doi:10.18699/vj20.679

Cited by 3 publications

(1 citation statement)

References 59 publications

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“…In Western Siberia, the isolated Pgt population is subdivided into two subpopulations (Omsk and Altai ones). The Omsk Pgt population is highly aggressive, and its pathotypes have a large number of virulence genes [19,20].…”

Section: Introductionmentioning

confidence: 99%

Nonhost Resistance of Thinopyrum ponticum to Puccinia graminis f. sp. tritici and the Effects of the Sr24, Sr25, and Sr26 Genes Introgressed to Wheat

Plotnikova

Knaub

Pozherukova

2023

IJPB

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The damage to wheat crops by stem rust poses a threat to the food security of the world’s population. The species Thinopyrum ponticum (Podpěra, 1902) (Z.-W. Liu and R.-C. Wang, 1993) is a non-host for the stem rust fungus Puccinia graminis f. sp. tritici Eriks. and Henn. (Pgt). The Sr24, Sr25, and Sr26 genes, transferred from the Th. ponticum to the wheat gene pool, protect cultivars from the disease in different regions of the world. The study of the non-host resistance (NHR) of Th. ponticum and the effects of the introgressed Sr24, Sr25, and Sr26 genes in wheat is important for breeding cultivars with durable resistance to stem rust. The aim of the research is to study the interaction of Pgt with Th. ponticum and common wheat lines with the Sr24, Sr25, and Sr26 genes, in addition to determining the role of ROS in resistance. Wheat lines with Sr24, Sr25, and Sr26 were resistant to the West Siberian Pgt population. Using cytological methods, it was found that the NHR of Th. ponticum and Sr24, Sr25, and Sr26 led to inhibition of the most inoculumdevelopment on the plant surface. This was mainly due to the suppression of the appressoria development and their death at the stage of penetration into the stomata. Upon contact of Pgt appressoria with stomatal guard cells, the generation of the superoxide anion O2•− was revealed. This interaction is similar to the stomatal immunity of Arabidopsis thaliana to non-pathogenic bacteria. The results of our studies show that the Sr24, Sr25, and Sr26 genes reproduce the action of some NHR mechanisms in wheat.

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Section: Introductionmentioning

confidence: 99%

Nonhost Resistance of Thinopyrum ponticum to Puccinia graminis f. sp. tritici and the Effects of the Sr24, Sr25, and Sr26 Genes Introgressed to Wheat

Plotnikova

Knaub

Pozherukova

2023

IJPB

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Molecular cytogenetic characteristics of new spring bread wheat introgressive lines resistant to stem rust

Baranova,

Adonina,

Sibikeev

2024

Vestn. VOGiS

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Anticipatory wheat breeding for pathogen resistance is key to preventing economically significant crop losses caused by diseases. Recently, the harmfulness of a dangerous wheat disease, stem rust, caused by Puccinia graminis f. sp. tritici, was increased in the main grain-producing regions of the Russian Federation. At the same time, importation of the Ug99 race (TTKSK) is still a possibility. In this regard, the transfer of effective resistance genes from related species to the bread wheat breeding material followed by the chromosomal localization of the introgressions and a marker analysis to identify known resistance genes is of great importance. In this work, a comprehensive analysis of ten spring bread wheat introgressive lines of the Federal Center of Agricultural Research of the South-East Region (L657, L664, L758, L935, L960, L968, L971, L995/1, L997 and L1110) was carried out. These lines were obtained with the participation of Triticum dicoccum, T. timopheevii, T. kiharae, Aegilops speltoides, Agropyron elongatum and Secale cereale. In this study, the lines were evaluated for resistance to the Ug99 race (TTKSK) in the Njoro, Kenya. Evaluation of introgression lines in the field for resistance to the Ug99 race (TTKSK) showed that four lines were immune, two were resistant, three were moderately resistant, and one had an intermediate type of response to infection. By cytogenetic analysis of these lines using fluorescent (FISH) and genomic (GISH) in situ hybridization, introgressions from Ae. speltoides (line L664), T. timopheevii (lines L758, L971, L995/1, L997 and L1110), Thinopyrum ponticum = Ag. elongatum (2n = 70) (L664, L758, L960, L971, L997 and L1110), as well as introgressions from T. dicoccum (L657 and L664), T. kiharae (L960) and S. cereale (L935 and L968) were detected. Molecular markers recommended for marker-oriented breeding were used to identify known resistance genes (Sr2, Sr25, Sr32, Sr1A.1R, Sr36, Sr38, Sr39 and Sr47). The Sr36 and Sr25 genes were observed in lines L997 and L1110, while line L664 had the Sr39+Sr47+Sr25 gene combination. In lines L935 and L968 with 3R(3D) substitution from S. cereale, gene resistance was presumably identified as SrSatu. Thus, highly resistant to both local populations of P. graminis and the Ug99 race, bread wheat lines are promising donors for the production of new varieties resistant to stem rust.

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What Was the Reason for the Durable Effect of Sr31 against Wheat Stem Rust?

et al. 2022

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Common wheat cultivars have been protected from stem rust for several decades worldwide by the Sr31 resistance gene transferred from Secale cereale L. (cv. Petkus). In 1998, Sr31 was overcome in Uganda by the Ug99 race of Puccinia graminis f. sp. tritici Eriks. & Henn. (Pgt). The Ug99 race and its derivatives have spread widely in Africa, neighboring regions and Europe. However, Sr31 remains effective in other areas of the world, including Russia. To breed wheat with durable resistance, it is promising to research the resistance mechanisms of nonhost species and introgressive cultivars. The aim of the research was to estimate the resistance of S. cereale and Triticum aestivum cultivars with Sr31 to stem rust and to study the mechanisms of incompatibility of Pgt with plants at the cellular level. The research was carried out in Western Siberia (Russia, Omsk region) in 2018–2022. Rye and wheat with Sr31 (cvs. Kavkaz, Seri 82, Bacanora (=Kauz’s’), NIL Thatcher TcLr26/Sr31) were resistant at the stages of seedling and adult plant, and cv. PWB343 was more susceptible to disease. Cytological studies have shown that Pgt died on the rye plants on the surface, and cv. Petkus intensively suppressed the development of the appressoria necessary to penetrate into tissues. Wheat cultivars inhibited the Pgt development mainly on the surface and while it attempted to penetrate into the stomata (pre-haustorial resistance). It has been demonstrated that Pgt has to adapt step-by-step to the surface and tissue properties for compatible interaction, which may be the reason for the durable effectiveness of Sr31.

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Challenges and prospects for developing genetic resistance in common wheat against stem rust in Western Siberia

Cited by 3 publications

References 59 publications

Nonhost Resistance of Thinopyrum ponticum to Puccinia graminis f. sp. tritici and the Effects of the Sr24, Sr25, and Sr26 Genes Introgressed to Wheat

Nonhost Resistance of Thinopyrum ponticum to Puccinia graminis f. sp. tritici and the Effects of the Sr24, Sr25, and Sr26 Genes Introgressed to Wheat

Molecular cytogenetic characteristics of new spring bread wheat introgressive lines resistant to stem rust

What Was the Reason for the Durable Effect of Sr31 against Wheat Stem Rust?

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