Work rules are an important guiding principle that regulates professional behaviour in organizational settings, however, the mechanism through which work rules affect physicians’ compassion satisfaction and career satisfaction remains unknown. Additionally, existing literature mainly focuses on direct associations, overlooking team support’s potential mediating role. This creates a research gap regarding team support as a mediator between work rules and both compassion and career satisfaction, requiring further scholarly investigation. Hence, the present study aims to investigate team support as a mediator between work rules and both compassion and career satisfaction in Obstetrics and Gynaecology (Obs and Gynae) physicians. A cross-sectional survey was distributed to 347 participants between 24 and 60 years (M = 31.22; SD = 6.81) in tertiary care hospitals. Participants were approached through purposive and convenient sampling techniques. A structural equation modelling through AMOS was analysed to study the complex interlinkages among the variables. The study found significant mediation of team support between work rules and career satisfaction (b = 0.077, 95% CI = 0.052, 0.106, P = 0.000). Also, an indirect significant path of team support is found between work rules and compassion satisfaction (b = 0.032, 95% CI = 0.020, 0.044, P = 0.000). Teamwork has far-reaching implications for healthcare professionals’ well-being, team dynamics, patient care quality, and the overall effectiveness of healthcare organizations. Filling this research gap enables healthcare professionals to foster more supportive work environment, benefiting both physicians and the patients they serve.