Challenges in Studying the Incorporation of Nanomaterials to Building Materials on Microbiological Models

Augustyniak, Adrian; Sikora, Paweł; Cendrowski, Krzysztof; Nawrotek, Paweł; Mijowska, Ewa; Stephan, Dietmar

doi:10.1007/978-3-030-17755-3_20

Cited by 6 publications

(6 citation statements)

References 87 publications

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“…Even though the incorporation of nanosized materials such as C-S-H, silica nanoparticles, carbon nanotubes, titanium dioxide, and others has been widely studied in the literature of the last two decades (Spinazzè et al 2016;Bossa et al 2017;Diamond et al 2017;Miller et al 2017), still, there are strong concerns regarding the handling and safety of using nanosized admixtures during cement-based composites production, as well as during the demolition works (Lee et al 2010;Van Broekhuizen et al 2011;Jones et al 2016;Strokova et al 2018;Torres-Carrasco et al 2019). There are many factors affecting the potentially hazardous effects of nanomaterials arising from their size, shape, chemical composition, the tendency to agglomerate, solubility, surface area and charge (Nawrotek and Augustyniak 2015;Miller et al 2017;Augustyniak et al 2019). While the toxicity of nanoparticles has been proven by many studies in laboratory conditions (Aruguete et al 2013;Mosselhy et al 2017;Díez-Pascual 2018), their toxicity under the mass production of cementitious composites may vary due to the fact that most of the nanosized admixtures are not stabilized in a working aqueous suspension.…”

Section: Introductionmentioning

confidence: 99%

The effects of calcium–silicate–hydrate (C–S–H) seeds on reference microorganisms

et al. 2020

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Building materials are constantly improved with various additives and admixtures in order to achieve goals ranging from obtaining increased durability or antimicrobial activity up to reducing the carbon footprint left by the cement production. Since nanomaterials were proposed for cement products, many studies explored the possibilities for their incorporation. One of the novel trends in studying these materials is evaluating their impact on living organisms, with the focus not only on toxicology but also on the application potential. Therefore, in this study, we investigated the effects of three types of calciumsilicate-hydrate (C-S-H) seeds on reference microorganisms in the scope of their basic physiology and primary metabolism. Shape, size and elemental composition of C-S-H seeds were also evaluated. The tests on the reference microorganisms have shown that the reaction to these nanomaterials can be specific and depends on the strain as well as the type of used nanomaterial. Furthermore, the presence of C-S-H seeds in the growth environment led to metabolic stimulation that resulted in faster growth, higher biochemical activity, and increased biofilm formation. Based on our findings, we conclude that even though C-S-H seeds have antimicrobial potential, they can be potentially used to promote the growth of selected microbial strains. This phenomenon could be further investigated towards the formation of beneficial biofilms on building materials.

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Section: Introductionmentioning

confidence: 99%

The effects of calcium–silicate–hydrate (C–S–H) seeds on reference microorganisms

et al. 2020

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“…However, the effects are in most cases described only with viability measures, often restricted to plate count methods or optical density measurements (Sikora et al 2016(Sikora et al , 2017. This approach may suggest a decrease in viability, although it does not describe the changes in bacterial physiology (Augustyniak et al 2019). Therefore, the literature also argued that it is necessary to propose a comprehensive approach for studying nanomaterials on microorganisms which will broaden the current perspective, frequently restricted to solely studying the toxicity (Nawrotek and Augustyniak 2015).…”

Section: Introductionmentioning

confidence: 99%

Basic physiology of Pseudomonas aeruginosa contacted with carbon nanocomposites

et al. 2022

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Experiments describing properties of nanomaterials on bacteria are frequently limited to the disk diffusion method or other end-point methods indicating viability or survival rate in plate count assay. Such experimental design does not show the dynamic changes in bacterial physiology, mainly when performed on reference microorganisms (Escherichia coli and Staphylococcus aureus). Testing other microorganisms, such as Pseudomonas aeruginosa, could provide novel insights into the microbial response to nanomaterials. Therefore, we aimed to test selected carbon nanomaterials and their components in a series of experiments describing the basic physiology of P. aeruginosa. Concentrations ranging from 15.625 to 1000 µg/mL were tested. The optical density of cultures, pigment production, respiration, growth curve analysis, and biofilming were tested. The results confirmed variability in the response of P. aeruginosa to tested nanostructures, depending on their concentration. The co-incubation with the nanostructures (in concentration 125 µg/mL) could inhibit the population growth (in most cases) or promote it in the case of graphene oxide. Furthermore, a specific concentration of a given nanomaterial could cause contradictory effects leading to stimulation or inhibition of pigmentation, an optical density of the cultures, or biofilm formation. We have found that particularly nanomaterials containing TiO2 could induce pigmentation in P. aeruginosa, which indicates the possibility of increased virulence. On the other hand, nanocomposites containing cobalt nanoparticles had the highest anti-bacterial potential when cobalt was displayed on the surface. Our approach revealed changes in respiration and growth dynamics that can be used to search for nanomaterials’ application in biotechnology.

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“…Despite the significant amount of research works and the first commercially available products on the market, occupational safety, and health-related issues regarding handling and exposure to nanoparticles are still unclear and fully understood. During various internal and external conditions over the life cycle of building structures, cementitious composites undergo a series of deterioration and weathering processes, thus the possibility of releasing of nanomaterials should be carefully studied (Lee et al 2010;Van Broekhuizen et al 2011;Jones et al 2016;Silva et al 2018;Giese et al 2018;Augustyniak et al 2019). Another aspect that was rarely considered is the interaction between various human-made materials and microorganisms, even though they have multiple meanings, e.g., for the sustainability of cementitious materials (Holden et al 2014;Sikora et al 2018a).…”

Section: Introductionmentioning

confidence: 99%

“…Nanomaterials were found to exhibit certain toxicity when analyzed in the laboratory scale; however, the assessed toxicity of particular NPs cannot be directly transferred from laboratory results to the real release scenarios. Since NPs are immobilized within the cement matrix, there is a high possibility that NPs during the deterioration of composite will be released together with matrix particles or in an agglomerated form (Nowack and Bucheli 2007;Mitrano et al 2015;Giese et al 2018;Augustyniak et al 2019). Moreover, obtaining the full dispersion of NPs when producing cementitious composites is a challenging issue.…”

Section: Introductionmentioning

confidence: 99%

Investigating the release of ZnO nanoparticles from cement mortars on microbiological models

et al. 2021

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Incorporating zinc oxide nanoparticles (ZnO NPs) into cement mortars may provide additional functions, e.g., self-cleaning and antibacterial or electroconductive ability. However, these NPs are also known for their potential toxicity. During the life cycle of a cement mortar, various abrasive forces cause the release of admixtures to the natural environment. The effect of the released NPs on model microorganisms has not been extensively studied. Previous studies have shown that nanomaterials may affect various microorganisms’ physiological responses, including changes in metabolic activity, biofilming, or growth rate. In this study, we have focused on evaluating the response of model microorganisms, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Candida albicans, towards ZnO nanoparticles released from cement mortars in different deterioration scenarios. The addition of ZnO nanoparticles to cement mortars had a noticeable effect on impeding the strength development. We have also detected that depending on the deterioration scenario, the release of ZnO nanoparticles was varied. Our studies have also shown that even though the release of nanoform ZnO could be limited by poor dispersion or the used filtration technique, the eluates have caused slight but statistically significant changes in the physiological features of studied microorganisms showing relatively low toxicity.

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Challenges in Studying the Incorporation of Nanomaterials to Building Materials on Microbiological Models

Cited by 6 publications

References 87 publications

The effects of calcium–silicate–hydrate (C–S–H) seeds on reference microorganisms

The effects of calcium–silicate–hydrate (C–S–H) seeds on reference microorganisms

Basic physiology of Pseudomonas aeruginosa contacted with carbon nanocomposites

Investigating the release of ZnO nanoparticles from cement mortars on microbiological models

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