The islands of São Tomé and Príncipe host extraordinary biodiversity that evolved over millions of years without human presence. In the fifteenth century, the colonization of the islands created a society of migrants, associated with extensive land-use change and generally low knowledge and stewardship of autochthonous biodiversity. Formal education became widely accessible after the country’s independence but the curriculum has never been aligned with the natural heritage of the islands. Informal environmental education started in the 1990s alongside the pioneer conservation initiatives involving the scientific community. In the last decade, these efforts have multiplied, in line with the need to engage and involve local actors to promote stewardship and ensure the success of conservation efforts. Some changes were made recently at a formal level with the inclusion of environmental education curricula and new manuals. In addition, several initiatives and improvements are being developed in the private education sector. However, limited access to resources for educators reduces motivation and capacity to implement longer-term improvements. Most environmental education activities are still promoted by NGOs and mainly focus on endemic or threatened species and target school-age audiences. Other initiatives focusing on specific demographic groups have provided interesting results but are more intermittent and their impacts have largely not yet been evaluated. Improving formal assessments for current and future projects to assess impacts and refine future approaches will be essential moving forward. In addition, ensuring the involvement of local actors, coordination between different initiatives, and the use of diversified approaches will ensure that environmental education engages the widest possible audiences.