Challenges in the Physical Characterization of Lipid Nanoparticles

Hallan, Supandeep Singh; Sguizzato, Maddalena; Esposito, Elisabetta; Cortesi, Rita

doi:10.3390/pharmaceutics13040549

Cited by 70 publications

(39 citation statements)

References 178 publications

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“…The viability of cells treated with charged liposomes was slightly higher than those treated with plain-LP. This effect could be ascribed to the possible electrostatic repulsion between the negative charge and the membrane macromolecules, leading to a reduced cell-liposome interaction with a subsequent reduction in the LP antiproliferative effect [49,50].…”

Section: In Vitro Effect Of Produced Liposomal Formulations On Cultur...mentioning

confidence: 99%

Manganese in Diagnostics: A Preformulatory Study

et al. 2022

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This investigation aims to find lipid-based nanosystems to be used as tools to deliver manganese for diagnostic purposes in multimodal imaging techniques. In particular, the study describes the production and characterization of aqueous dispersions of anionic liposomes as delivery systems for two model manganese-based compounds, namely manganese chloride and manganese acetylacetonate. Negatively charged liposomes were obtained using four different anionic surfactants, namely sodium docusate (SD), N-lauroylsarcosine (NLS), Protelan AG8 (PAG) and sodium lauroyl lactylate (SLL). Liposomes were produced by the direct hydration method followed by extrusion and characterized in terms of size, polydispersity, surface charge and stability over time. After extrusion, liposomes are homogeneous and monodispersed with an average diameter not exceeding 200 nm and a negative surface charge as confirmed by ζ potential measurement. Moreover, as indicated by atomic absorption spectroscopy analyses, the loading of manganese-based compounds was almost quantitative. Liposomes containing NLS or SLL were the most stable over time and the presence of manganese-based compounds did not affect their size distribution. Liposomes containing PAG and SD were instable and therefore discarded. The in vitro cytotoxicity of the selected anionic liposomes was evaluated by MTT assay on human keratinocyte. The obtained results highlighted that the toxicity of the formulations is dose dependent.

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Section: In Vitro Effect Of Produced Liposomal Formulations On Cultur...mentioning

confidence: 99%

Manganese in Diagnostics: A Preformulatory Study

et al. 2022

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“…For example, none of the nanocarriers associated with CPPs have so far been approved for clinical studies. Evidently, besides overcoming membrane barriers, a string of challenges remain that need to be tackled for peptide nanocarriers to make a breakthrough in clinical application whereas lipid-based formulations, for all their drawbacks [277], are being used in the field of gene therapy and as a delivery vehicle in mRNA-based vaccines [278]. Short circulation half-lives, inadequate biodistribution, and poor chemical and physical serum stability, especially susceptibility to proteolytic degradation associated with off-target nucleic acid release, hamper clinical translation of peptide-based nanocarriers.…”

Section: Discussionmentioning

confidence: 99%

Peptide-Assisted Nucleic Acid Delivery Systems on the Rise

Tarvirdipour

Skowicki

Schoenenberger

et al. 2021

IJMS

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Concerns associated with nanocarriers’ therapeutic efficacy and side effects have led to the development of strategies to advance them into targeted and responsive delivery systems. Owing to their bioactivity and biocompatibility, peptides play a key role in these strategies and, thus, have been extensively studied in nanomedicine. Peptide-based nanocarriers, in particular, have burgeoned with advances in purely peptidic structures and in combinations of peptides, both native and modified, with polymers, lipids, and inorganic nanoparticles. In this review, we summarize advances on peptides promoting gene delivery systems. The efficacy of nucleic acid therapies largely depends on cell internalization and the delivery to subcellular organelles. Hence, the review focuses on nanocarriers where peptides are pivotal in ferrying nucleic acids to their site of action, with a special emphasis on peptides that assist anionic, water-soluble nucleic acids in crossing the membrane barriers they encounter on their way to efficient function. In a second part, we address how peptides advance nanoassembly delivery tools, such that they navigate delivery barriers and release their nucleic acid cargo at specific sites in a controlled fashion.

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“…Among lipid nanoparticles, solid lipid nanoparticles (SLNs) and nanostructured lipid carriers (NLCs) are colloidal carriers exploiting many effects on skin, and therefore are suitable for skin applications. Figure 5 shows exemplary cryo-TEM images of tristearin SLNs (c) and NLCs (d) [ 99 , 100 , 101 ]. It has been demonstrated that the solid core of these nanoparticles is able to better sustain and control the release of drug as compared to emulsions or other vesicles, such as liposomes [ 96 ].…”

Section: Lipid-based Nanosystemsmentioning

confidence: 99%

Lipid-Based Nanosystems as a Tool to Overcome Skin Barrier

Sguizzato

Esposito

Cortesi

2021

IJMS

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Skin may be affected by many disorders that can be treated by topical applications of drugs on the action site. With the advent of nanotechnologies, new efficient delivery systems have been developed. Particularly, lipid-based nanosystems such as liposomes, ethosomes, transferosomes, solid lipid nanoparticles, nanostructured lipid carriers, cubosomes, and monoolein aqueous dispersions have been proposed for cutaneous application, reaching in some cases the market or clinical trials. This review aims to provide an overview of the different lipid-based nanosystems, focusing on their use for topical application. Particularly, biocompatible nanosystems able to dissolve lipophilic compounds and to control the release of carried drug, possibly reducing side effects, are described. Notably, the rationale to topically administer antioxidant molecules by lipid nanocarriers is described. Indeed, the structural similarity between the nanosystem lipid matrix and the skin lipids allows the achievement of a transdermal effect. Surely, more research is required to better understand the mechanism of interaction between lipid-based nanosystems and skin. However, this attempt to summarize and highlight the possibilities offered by lipid-based nanosystems could help the scientific community to take advantage of the benefits derived from this kind of nanosystem.

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Challenges in the Physical Characterization of Lipid Nanoparticles

Cited by 70 publications

References 178 publications

Manganese in Diagnostics: A Preformulatory Study

Manganese in Diagnostics: A Preformulatory Study

Peptide-Assisted Nucleic Acid Delivery Systems on the Rise

Lipid-Based Nanosystems as a Tool to Overcome Skin Barrier

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