Challenges in the production of itaconic acid by metabolically engineered<i>Escherichia coli</i>

Yamamoto, Kouhei; Nagata, Kenichi; Ohara, Hirotaka; Aso, Yoichi

doi:10.1080/21655979.2015.1068471

Cited by 7 publications

(5 citation statements)

References 22 publications

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“…Numerous reviews have summarized the attempts to improve the various IA production technologies [27,28]. Many researchers have actively evaluated different industrially significant microorganisms that synthesize IA [29,30], and also specific improvements through genetic engineering of various strains for higher IA production [31,32]. Due to the increased global marketing potential of IA, biotechnological production was also evaluated [33].…”

Section: Introductionmentioning

confidence: 99%

Biomass-Derived Production of Itaconic Acid as a Building Block in Specialty Polymers

2019

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Biomass, the only source of renewable organic carbon on Earth, offers an efficient substrate for bio-based organic acid production as an alternative to the leading petrochemical industry based on non-renewable resources. Itaconic acid (IA) is one of the most important organic acids that can be obtained from lignocellulose biomass. IA, a 5-C dicarboxylic acid, is a promising platform chemical with extensive applications; therefore, it is included in the top 12 building block chemicals by the US Department of Energy. Biotechnologically, IA production can take place through fermentation with fungi like Aspergillus terreus and Ustilago maydis strains or with metabolically engineered bacteria like Escherichia coli and Corynebacterium glutamicum. Bio-based IA represents a feasible substitute for petrochemically produced acrylic acid, paints, varnishes, biodegradable polymers, and other different organic compounds. IA and its derivatives, due to their trifunctional structure, support the synthesis of a wide range of innovative polymers through crosslinking, with applications in special hydrogels for water decontamination, targeted drug delivery (especially in cancer treatment), smart nanohydrogels in food applications, coatings, and elastomers. The present review summarizes the latest research regarding major IA production pathways, metabolic engineering procedures, and the synthesis and applications of novel polymeric materials.

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Section: Introductionmentioning

confidence: 99%

Biomass-Derived Production of Itaconic Acid as a Building Block in Specialty Polymers

2019

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“…A similar result was previously found when IA was produced in the resting cells of a recombinant E. coli (Yamamoto et al . 2015). These results suggest that the yield of 9‐HHIA and 10‐HHIA from glucose would be improved by using the resting cells of A. niger S17‐5.…”

Section: Discussionmentioning

confidence: 99%

Caprylic acid enhances hydroxyhexylitaconic acid production in Aspergillus niger S17‐5

et al. 2020

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Aim Aspergillus niger S17‐5 produces two alkylitaconic acids, 9‐hydroxyhexylitaconic acid (9‐HHIA) and 10‐hydroxyhexylitaconic acid (10‐HHIA), which have cytotoxic and polymer building block properties. In this study, we characterized the production of 9‐HHIA and 10‐HHIA by addition of their expected precursor, caprylic acid, to a culture of A. niger S17‐5, and demonstrated batch fermentation of 9‐HHIA and 10‐HHIA in a jar fermenter with DO‐stat. Methods and Results Production titres of 9‐HHIA and 10‐HHIA from 3% glucose in a flask after 25 days cultivation were 0·35 and 1·01 g l−1 respectively. Addition of 0·22 g l−1 of caprylic acid to a suspension of resting cells of A. niger S17‐5 led to 32% enhancement of total 9‐HHIA and 10‐HHIA production compared to no addition. No enhancement of the production of 9‐HHIA or 10‐HHIA by the addition of oxaloacetic acid was observed. Addition of caprylic acid to the culture at mid‐growth phase was more suitable for 9‐HHIA and 10‐HHIA production due to less cell growth inhibition by caprylic acid. DO‐stat batch fermentation with 3% glucose and 14·4 g l−1 of caprylic acid in a 1·5 l jar fermenter resulted in the production titres of 9‐HHIA and 10‐HHIA being 0·48 and 1·54 g l−1 respectively after 10 days of cultivation. Conclusions Addition of caprylic acid to the culture of A. niger S17‐5 enhances 9‐HHIA and 10‐HHIA production. Significance and Impact of the Study These results suggest that 9‐HHIA and 10‐HHIA are synthesized with octanoyl‐CoA derived from caprylic acid, and that the supply of octanoyl‐CoA is a rate‐limiting step in 9‐HHIA and 10‐HHIA production. To the best of our knowledge, this is the first report regarding the fermentation of naturally occurring itaconic acid derivatives in a jar fermenter.

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“…The transformants of E. coli were cultured in Luria Bertani (LB) broth containing appropriate antibiotics for cell growth and protein expression. Then, the reconstructed strains for ITA production were grown at 30 °C on a rotary shaker at 200 rpm in minimal media (MM) broth [ 25 ]. To quantify ITA production, samples were collected from 1-mL of culture and filtered using a 0.45-μm filtration membrane.…”

Section: Methodsmentioning

confidence: 99%

A CRISPRi mediated self-inducible system for dynamic regulation of TCA cycle and improvement of itaconic acid production in Escherichia coli

Zhao

Li²,

Wang³

et al. 2022

Synthetic and Systems Biotechnology

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Challenges in the production of itaconic acid by metabolically engineeredEscherichia coli

Cited by 7 publications

References 22 publications

Biomass-Derived Production of Itaconic Acid as a Building Block in Specialty Polymers

Biomass-Derived Production of Itaconic Acid as a Building Block in Specialty Polymers

Caprylic acid enhances hydroxyhexylitaconic acid production in Aspergillus niger S17‐5

A CRISPRi mediated self-inducible system for dynamic regulation of TCA cycle and improvement of itaconic acid production in Escherichia coli

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