Challenges of scaling-up PHA production from waste streams. A review

Rodríguez-Pérez, Santiago; Serrano, Antonio; Pantión, Alba A.; Alonso-Fariñas, Bernabé

doi:10.1016/j.jenvman.2017.09.083

Cited by 220 publications

(112 citation statements)

References 68 publications

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“…Fed‐batch cultures have been used to evaluate the behaviour of the strain during PHA production and to optimize the fermentation to try to achieve high cell density cultures. As mentioned before, PHA is an intracellular product, so it is very important to obtain high cell density cultures as a step prior to the industrial scale‐up especially using waste streams as substrates (Rodriguez‐Perez et al ., ). A first fermentation was performed using the MM3 medium supplemented with magnesium sulfate and ammonium sulfate to ensure the nutrients needed by the strain during the first phase of the fermentation.…”

Section: Resultsmentioning

confidence: 99%

In silico prospection of microorganisms to produce polyhydroxyalkanoate from whey: Caulobacter segnis DSM 29236 as a suitable industrial strain

Bustamante

Segarra

Tortajada

et al. 2019

Microbial Biotechnology

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Summary Polyhydroxyalkanoates ( PHA s) are polyesters of microbial origin that can be synthesized by prokaryotes from noble sugars or lipids and from complex renewable substrates. They are an attractive alternative to conventional plastics because they are biodegradable and can be produced from renewable resources, such as the surplus of whey from dairy companies. After an in silico screening to search for ß‐galactosidase and PHA polymerase genes, several bacteria were identified as potential PHA producers from whey based on their ability to hydrolyse lactose. Among them, Caulobacter segnis DSM 29236 was selected as a suitable strain to develop a process for whey surplus valorization. This microorganism accumulated 31.5% of cell dry weight ( CDW ) of poly(3‐hydroxybutyrate) ( PHB ) with a titre of 1.5 g l −1 in batch assays. Moreover, the strain accumulated 37% of CDW of PHB and 9.3 g l −1 in fed‐batch mode of operation. This study reveals this species as a PHA producer and experimentally validates the in silico bioprospecting strategy for selecting microorganisms for waste re‐valorization.

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Section: Resultsmentioning

confidence: 99%

In silico prospection of microorganisms to produce polyhydroxyalkanoate from whey: Caulobacter segnis DSM 29236 as a suitable industrial strain

Bustamante

Segarra

Tortajada

et al. 2019

Microbial Biotechnology

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“…Polar and disperse contributions to the surface energy and surface tension, respectively, were combined. Therefore (3,4):…”

Section: Surface Free Energy Of Polyester Samplesmentioning

confidence: 99%

“…Currently, efforts are made to reduce the amount of this waste by replacing petrochemical polymers by easily degradable polymer materials obtained from biorenewable sources (such as alginate, cellulose, chitosan, collagen carrageenan, corn zein, soy, starches). Sustainable packaging materials are often more functional and environmentally friendly then traditional plastic [3,4].…”

Section: Introductionmentioning

confidence: 99%

Biodegradable Polyester Materials Containing Gallates

Latos‐Brozio

Masek

2020

Polymers

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Gallates are widely used as antioxidants in the food and cosmetics industries. The purpose of the study was to obtain pro-ecological materials based on biodegradable polyesters, such as polylactide (PLA) and polyhydroxyalkanoate (PHA), and gallates. Gallates (ethyl, propyl, octyl, and lauryl) have not been used so far in biodegradable polymers as stabilizers and indicators of aging. This manuscript examines the properties of gallates such as antioxidant capacity and thermal stability. This paper also presents the following analyses of polymer materials: specific migration of gallates from polymers, SEM microscopy, differential scanning calorimetry (DSC), wide-angle X-ray diffraction, mechanical properties, surface free energy, and determination of change of color after controlled UV exposure, thermooxidation, and weathering. All gallates showed strong antioxidant properties and good thermal stability. Due to these properties, in particular their high oxidation temperature, gallates can be successfully used as polyester stabilizers. Biodegradable polyesters containing gallates can be an environmentally friendly alternative to petrochemical packaging materials.Increasing attention is being paid to the fact that, besides polymer matrices, also processing additives should be environmentally and consumer-friendly, especially those for food packaging and medical applications materials. Plant-based substances are more and more commonly proposed as pro-ecological stabilizers for polymeric materials, dyes, antibacterial/antifungal agents, freshness indicators of packaged food products, as well as indicators of the life time of polymers [12][13][14][15][16][17][18]. The combination of biodegradable polyesters with substances of plant origin and their derivatives can allow to obtain a new generation of polymeric materials that will be fully degradable and safe for consumers [19,20].The aim of study was obtained pro-ecological, biodegradable polyester materials containing gallates. Chemically, gallates are alkyl esters of 3,4,5-trihydroxybenzoic acid, the naturally occurring gallic acid. Gallic acid is one of the most abundant phenolic acids in the plant kingdom. It has been isolated from different plants such as oaks and pomegranates. Due to its free-radical scavenging and antioxidant properties, gallic acid and its derivatives, such as lauryl gallate, propyl gallate, octyl gallate, tetradecyl gallate, and hexadecyl gallate, can inhibit oxidation and rancidity in oils and fats [21][22][23][24][25][26].Gallates are antioxidants used as preservatives in the food, cosmetics, and pharmaceutical industries. In industry, synthetic derivatives of gallic acid, i.e., propyl gallate (E310), octyl gallate (E311), and dodecyl (lauryl) gallate (E312), are commonly used [27,28].In this manuscript, gallates were used as environmentally and consumer-friendly stabilizers to control the lifetime of biodegradable polyesters. Such an application has not been described in the literature so far and is a scientific novelty. It seems that the pr...

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“…This includes the quest for new wild type and genetically modified microbes, development of a process design adapted to the kinetic characteristics PHA production, and ecologically benign and efficient techniques for separation of PHA and residual non-PHA biomass. Only the synopsis of these steps allows ecologically feasible and sustainable manufacturing of PHA (6,37,38). Fig.…”

Section: Polyhydroxyalkanoates (Pha) -A Biological Alternativementioning

confidence: 99%

Advanced approaches to produce polyhydroxyalkanoate (PHA) biopolyesters in a sustainable and economic fashion

Koller

Braunegg

2018

The EuroBiotech Journal

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Polyhydroxyalkanoates (PHA), the only group of "bioplastics" sensu stricto, are accumulated by various prokaryotes as intracellular "carbonosomes". When exposed to exogenous stress or starvation, presence of these microbial polyoxoesters of hydroxyalkanoates assists microbes to survive."Bioplastics" such as PHA must be competitive with petrochemically manufactured plastics both in terms of material quality and manufacturing economics. Cost-effectiveness calculations clearly show that PHA production costs, in addition to bioreactor equipment and downstream technology, are mainly due to raw material costs. The reason for this is PHA production on an industrial scale currently relying on expensive, nutritionally relevant "1 st -generation feedstocks", such as like glucose, starch or edible oils. As a way out, carbon-rich industrial waste streams ("2 nd -generation feedstocks") can be used that are not in competition with the supply of food; this strategy not only reduces PHA production costs, but can also make a significant contribution to safeguarding food supplies in various disadvantaged parts of the world. This approach increases the economics of PHA production, improves the sustainability of the entire lifecycle of these materials, and makes them unassailable from an ethical perspective.In this context, our EU-funded projects ANIMPOL and WHEYPOL, carried out by collaborative consortia of academic and industrial partners, successfully developed PHA production processes, which resort to waste streams amply available in Europe. As real 2 nd -generation feedstocks", waste lipids and crude glycerol from animal-processing and biodiesel industry, and surplus whey from dairy and cheese making industry were used in these processes. Cost estimations made by our project partners determine PHA production prices below 3 € (WHEYPOL) and even less than 2 € (ANIMPOL), respectively, per kg; these values already reach the benchmark of economic feasibility.The presented studies clearly show that the use of selected high-carbon waste streams of (agro)industrial origin contributes significantly to the cost-effectiveness and sustainability of PHA biopolyester production on an industrial scale.

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Challenges of scaling-up PHA production from waste streams. A review

Cited by 220 publications

References 68 publications

In silico prospection of microorganisms to produce polyhydroxyalkanoate from whey: Caulobacter segnis DSM 29236 as a suitable industrial strain

In silico prospection of microorganisms to produce polyhydroxyalkanoate from whey: Caulobacter segnis DSM 29236 as a suitable industrial strain

Biodegradable Polyester Materials Containing Gallates

Advanced approaches to produce polyhydroxyalkanoate (PHA) biopolyesters in a sustainable and economic fashion

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