Challenges of Spectrofluorometry Part 3: Sample-Specific Concerns

English, Caitlyn; Kitzhaber, Zechariah; Williams, Joshua; Myrick, Michael L.

doi:10.56530/spectroscopy.xh2276b4

Spectroscopy

2021

DOI: 10.56530/spectroscopy.xh2276b4

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Challenges of Spectrofluorometry Part 3: Sample-Specific Concerns

Caitlyn English

Zechariah Kitzhaber

Joshua Williams

et al.

Abstract: The first installment of this three-part series provided a procedure for quickly recording acceptable fluorescence spectra with classic commercial spectrofluorometers for most samples in common 1-cm pathlength cuvettes. Part 2 described how to correct for instrument response so data can be compared properly between laboratories. This part describes sample-specific concerns that could require modifications of our basic procedure to prevent our data from being inconsistent.

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2024

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A Low-Cost, Portable, Multi-Cancer Screening Device Based on a Ratio Fluorometry and Signal Correlation Technique

Alghamdi,

Aldhaheri

2024

Biosensors

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The autofluorescence of erythrocyte porphyrins has emerged as a potential method for multi-cancer early detection (MCED). With this method’s dependence on research-grade spectrofluorometers, significant improvements in instrumentation are necessary to translate its potential into clinical practice, as with any promising medical technology. To fill this gap, in this paper, we present an automated ratio porphyrin analyzer for cancer screening (ARPA-CS), a low-cost, portable, and automated instrument for MCED via the ratio fluorometry of porphyrins. The ARPA-CS aims to facilitate cancer screening in an inexpensive, rapid, non-invasive, and reasonably accurate manner for use in primary clinics or at point of care. To accomplish this, the ARPA-CS uses an ultraviolet-excited optical apparatus for ratio fluorometry that features two photodetectors for detection at 590 and 630 nm. Additionally, it incorporates a synchronous detector for the precision measurement of signals based on the Walsh-ordered Walsh–Hadamard transform (WHT)w and circular shift. To estimate its single-photodetector capability, we established a linear calibration curve for the ARBA-CS exceeding four orders of magnitude with a linearity of up to 0.992 and a low detection limit of 0.296 µg/mL for riboflavin. The ARPA-CS also exhibited excellent repeatability (0.21%) and stability (0.60%). Moreover, the ratio fluorometry of three serially diluted standard solutions of riboflavin yielded a ratio of 0.4, which agrees with that expected based on the known emission spectra of riboflavin. Additionally, the ratio fluorometry of the porphyrin solution yielded a ratio of 49.82, which was ascribed to the predominant concentration of protoporphyrin IX in the brown eggshells, as confirmed in several studies. This study validates this instrument for the ratio fluorometry of porphyrins as a biomarker for MCED. Nevertheless, large and well-designed clinical trials are necessary to further elaborate more on this matter.

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A Low-Cost, Portable, Multi-Cancer Screening Device Based on a Ratio Fluorometry and Signal Correlation Technique

Alghamdi,

Aldhaheri

2024

Biosensors

View full text Add to dashboard Cite

show abstract

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Challenges of Spectrofluorometry Part 3: Sample-Specific Concerns

Cited by 1 publication

References 26 publications

A Low-Cost, Portable, Multi-Cancer Screening Device Based on a Ratio Fluorometry and Signal Correlation Technique

A Low-Cost, Portable, Multi-Cancer Screening Device Based on a Ratio Fluorometry and Signal Correlation Technique

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