Challenges With the Development of Biomaterials for Sustainable Tissue Engineering

Williams, David

doi:10.3389/fbioe.2019.00127

Cited by 240 publications

(150 citation statements)

References 71 publications

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“…Ideal biomedical materials for implantation surgeries are endowed with characteristics, such as strength, easy molding and suturability, absence of cytotoxicity, biocompatibility, radiolucency, sterile availability, and, not last, inexpensiveness and sustainability [11,22,70]. TRICOL bovine and porcine pericardia, manufactured with a cost-effective decellularization procedure, possess all these features.…”

Section: Discussionmentioning

confidence: 99%

A Comprehensive Comparison of Bovine and Porcine Decellularized Pericardia: New Insights for Surgical Applications

et al. 2020

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Xenogeneic pericardium-based substitutes are employed for several surgical indications after chemical shielding, limiting their biocompatibility and therapeutic durability. Adverse responses to these replacements might be prevented by tissue decellularization, ideally removing cells and preserving the original extracellular matrix (ECM). The aim of this study was to compare the mostly applied pericardia in clinics, i.e., bovine and porcine tissues, after their decellularization, and obtain new insights for their possible surgical use. Bovine and porcine pericardia were submitted to TRICOL decellularization, based on osmotic shock, detergents and nuclease treatment. TRICOL procedure resulted in being effective in cell removal and preservation of ECM architecture of both species’ scaffolds. Collagen and elastin were retained but glycosaminoglycans were reduced, significantly for bovine scaffolds. Tissue hydration was varied by decellularization, with a rise for bovine pericardia and a decrease for porcine ones. TRICOL significantly increased porcine pericardial thickness, while a non-significant reduction was observed for the bovine counterpart. The protein secondary structure and thermal denaturation profile of both species’ scaffolds were unaltered. Both pericardial tissues showed augmented biomechanical compliance after decellularization. The ECM bioactivity of bovine and porcine pericardia was unaffected by decellularization, sustaining viability and proliferation of human mesenchymal stem cells and endothelial cells. In conclusion, decellularized bovine and porcine pericardia demonstrate possessing the characteristics that are suitable for the creation of novel scaffolds for reconstruction or replacement: differences in water content, thickness and glycosaminoglycans might influence some of their biomechanical properties and, hence, their indication for surgical use.

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Section: Discussionmentioning

confidence: 99%

A Comprehensive Comparison of Bovine and Porcine Decellularized Pericardia: New Insights for Surgical Applications

et al. 2020

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“…Nevertheless, the specified features of biomaterials (e.g., pore size or mechanical strength) are closely associated with their future applications. For instance, biomaterials for skin TE may possess lower mechanical strength compared to scaffolds dedicated for bone TE [9][10][11]13,17,18,31,33,196,[199][200][201].…”

Section: Application Of Polymer Scaffolds Modified With Proteins Andmentioning

confidence: 99%

“…Scaffolds are three-dimensional (3D) matrices, which mimic native extracellular matrix (ECM) to support cell growth. Therefore, they should be biocompatible, biodegradable at the desired rate, porous, and mechanically stable [6,[9][10][11]. Considering these requirements, the scaffolds based on natural and synthetic polymers alone, as well as composites, exhibit the highest biomedical potential [12][13][14].…”

Section: Introduction: the Role Of Proteins And Peptides In Tementioning

confidence: 99%

Proteins and Peptides as Important Modifiers of the Polymer Scaffolds for Tissue Engineering Applications—A Review

2020

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Polymer scaffolds constitute a very interesting strategy for tissue engineering. Even though they are generally non-toxic, in some cases, they may not provide suitable support for cell adhesion, proliferation, and differentiation, which decelerates tissue regeneration. To improve biological properties, scaffolds are frequently enriched with bioactive molecules, inter alia extracellular matrix proteins, adhesive peptides, growth factors, hormones, and cytokines. Although there are many papers describing synthesis and properties of polymer scaffolds enriched with proteins or peptides, few reviews comprehensively summarize these bioactive molecules. Thus, this review presents the current knowledge about the most important proteins and peptides used for modification of polymer scaffolds for tissue engineering. This paper also describes the influence of addition of proteins and peptides on physicochemical, mechanical, and biological properties of polymer scaffolds. Moreover, this article sums up the major applications of some biodegradable natural and synthetic polymer scaffolds modified with proteins and peptides, which have been developed within the past five years.

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“…These scaffold-based platforms rely on the mechanobiology concept by which biomaterials provide the 3D-structure of native tissues and through their chemical/physical properties are able to specifically steer stem cells toward tissue-specific phenotypes [14]. As a matter of fact, the modulation of physical and chemical properties of biomaterials such as architecture, shape, mechanical properties, and surface structure, have been showed to control the biological responses of stem cells (both stemness and differentiation properties) [113,115,116]. Many types of natural or synthetic biomaterials, such as polymers, ceramics, bio-glass, composites, or seldom metals [37,[117][118][119][120][121][122][123], have been combined with different types of stem cells in order to faithfully recapitulate key environmental signals (thus allowing finer cell/tissue models [11,124]) and elucidate complex biological phenomena such as stem cell reprogramming pathways and determination processes [14].…”

Section: Ex Vivo Stem Cell-based Systems: Bio-hybrid Models For Tissumentioning

confidence: 99%

Harnessing the Potential of Stem Cells for Disease Modeling: Progress and Promises

Argentati

Tortorella

Bazzucchi

et al. 2020

JPM

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Ex vivo cell/tissue-based models are an essential step in the workflow of pathophysiology studies, assay development, disease modeling, drug discovery, and development of personalized therapeutic strategies. For these purposes, both scientific and pharmaceutical research have adopted ex vivo stem cell models because of their better predictive power. As matter of a fact, the advancing in isolation and in vitro expansion protocols for culturing autologous human stem cells, and the standardization of methods for generating patient-derived induced pluripotent stem cells has made feasible to generate and investigate human cellular disease models with even greater speed and efficiency. Furthermore, the potential of stem cells on generating more complex systems, such as scaffold-cell models, organoids, or organ-on-a-chip, allowed to overcome the limitations of the two-dimensional culture systems as well as to better mimic tissues structures and functions. Finally, the advent of genome-editing/gene therapy technologies had a great impact on the generation of more proficient stem cell-disease models and on establishing an effective therapeutic treatment. In this review, we discuss important breakthroughs of stem cell-based models highlighting current directions, advantages, and limitations and point out the need to combine experimental biology with computational tools able to describe complex biological systems and deliver results or predictions in the context of personalized medicine.Since their establishment, cell cultures have been proven to be the first and most powerful tool for the in vitro investigation of cell biology, from basic research to more complex translational approaches [6]. Classical two-dimensional (2D)-cultures usually consist of a unique type of cells (primary or continuous cultures) that grow in tissue culture polystyrene as adherent monolayers or in floating suspension, both in culture media that provide essential nutrients and growth factors. 2D-strategies are widely used to obtain a large number of cells, thus allowing the in vitro study of molecular mechanisms and development of metabolic assays [7-9]. However, due to their inability to recreate the in vivo complexity of the biological environment, they are not suitable for accurate disease modeling. These limitations have been overcome by the development of three-dimensional (3D)-cell cultures systems [10]. The rationale design of 3D-cell cultures is to harvest cells in microstructures that resemble tissues or organs shape and organization, thus allowing better cell-to-cell/cell-environment contacts and signaling crosstalk [11][12][13][14][15], essential events for tissues correct development and functioning [14,15]. The 3D-cell culture strategy is articulated in many different methods and techniques that can be grouped in scaffold-based or non-scaffold based platforms, each with particular advantages and disadvantages that make them useful for different applications [10,[16][17][18][19]. 3D-cell culture strategies are supported by the in silico...

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Challenges With the Development of Biomaterials for Sustainable Tissue Engineering

Cited by 240 publications

References 71 publications

A Comprehensive Comparison of Bovine and Porcine Decellularized Pericardia: New Insights for Surgical Applications

A Comprehensive Comparison of Bovine and Porcine Decellularized Pericardia: New Insights for Surgical Applications

Proteins and Peptides as Important Modifiers of the Polymer Scaffolds for Tissue Engineering Applications—A Review

Harnessing the Potential of Stem Cells for Disease Modeling: Progress and Promises

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