Challenging and Rare DNA Profiling of Burned Corpses at the State Forensic Science Laboratory of Jharkhand

Abstract

Burning of corpses, most common in, the criminals and heinous crime cases and for the destruction of evidence more corpses are burnt in cremation often for destruction of evidence to obscure the crime scene and buried in boxed or dumped in the river or the graveyard. It's very cost effective reason to hide crime In the process of cremation temperatures about to 870-980°C (1,600-1,800°F) for 60-80 min or longer, after burning the dead body only leaving severely burnt teeth and some remains of long charred bones. In various criminal and civil cases, there is a requirement of DNA profiling for ascertained identity of the person and also help to solve the paternity-related issues. In the present study, we present autosomal DNA profiling of 12 corpses as the sample (legally known as exhibits) from the crime scene, one of the very sensational cases of state Jharkhand of India. The DNA profiling test performs in state forensic science laboratory Jharkhand. DNA extraction from charred bones, the human nuclear DNA was tested by highly sensitive multiplex PCR and quantified via real-time PCR; gene typing was done using the AmpFISTR Identifier Plus ® kit.

Importance of DNA profiling in sexual assault case: A case study

“…Forensic DNA profiling uses short tandem repeat (STR) markers to determine human identity. Commercially available kits have been accepted on a global scale for multiplex PCR for biological samples [5].…”