Chang's meaning of capacitation: A molecular perspective

Gervasi, María Gracia; Visconti, Pablo E.

doi:10.1002/mrd.22663

Cited by 133 publications

(123 citation statements)

References 173 publications

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“…Capacitation is a series of morphological and metabolic changes that is necessary for sperm to achieve fertilizing ability [1]. In present study, we found that the value of phospho-Thr172-AMPK increased when goat sperm were exposed to capacitation medium.…”

Section: Discussionsupporting

confidence: 55%

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5’-AMP-Activated Protein Kinase Regulates Goat Sperm Functions via Energy Metabolism In Vitro

Zhu

et al. 2018

Cell Physiol Biochem

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Background/Aims: ATP is essential for mammalian sperm to survive and maintain fertilizing capacity. AMP-activated protein kinase (AMPK) is a sensor of cellular energy status. The aims of the present study were to explore the localization of AMPK in goat sperm and to investigate whether and how AMPK regulates sperm functions in vitro. Methods: Sperm were treated with AMPK modulators (AICAR, metformin and Compound C) during incubation. Sperm motility was assessed with a computer-assisted spermatozoa analysis system (CASA). Membrane integrity, acrosome reaction and mitochondrial membrane potentials were detected by SYBR-14/PI, FITC-PNA and JC-1 staining, respectively. And the lactate content, ATP content, AMPK activity, activity of pyruvate kinase (PK) and lactate dehydrogenase (LDH) were also measured with the commercial assay kits. Immunofluorescence staining was used to analyze the distribution of PK, LDH, AMPK and phospho-Thr172-AMPK in sperm. The role of AMPK was further studied during induction of capacitation and acrosome reaction. Results: We found that AMPKα was localized in the entire acrosomal region, the midpiece and the flagellum, while the phospho-Thr172-AMPK was distributed in the head, the midpiece and flagellum. Activation of AMPK by AICAR and metformin significantly improved sperm motility, membrane integrity and acrosome reaction, largely maintained sperm mitochondrial membrane potentials, lactate content and ATP content, and enhanced the activity of AMPK, PK and LDH, whereas inhibition by Compound C triggered the converse effects. Moreover, PK was localized in the acrosomal area and the midpiece, while LDH was distributed in the tail. Induction of capacitation and acrosome reaction led to AMPK phosphorylation. AMPK phosphorylation regulated the activity of energetic enzymes. Conclusion: This study for the first time provides evidence that AMPK governs goat sperm functions through energy metabolism in vitro. This finding will help to improve assisted reproductive techniques in goats and the other species.

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Section: Discussionsupporting

confidence: 55%

“…Capacitation is a process in which sperm undergo biochemical changes before fertilizing eggs [1]. The protein tyrosine phosphorylation is one of the most important intracellular signaling events regarded as a meaningful indicator for capacitation [2].…”

Section: Introductionmentioning

confidence: 99%

5’-AMP-Activated Protein Kinase Regulates Goat Sperm Functions via Energy Metabolism In Vitro

Zhu

et al. 2018

Cell Physiol Biochem

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“…Sperm capacitation is described as the functional modifications that confer spermatozoa their ability to fertilize an egg. Capacitation encompasses the faculty of spermatozoa to attach to the zona pellucida and consequently experience the acrosome reaction (AR), present hypermotility, and develop the ability to adhere and fuse with the egg membrane . Actin cytoskeleton remodeling is an indispensable event for the fertilization process .…”

Section: Introductionmentioning

confidence: 99%

Rac1 is necessary for capacitation and acrosome reaction in guinea pig spermatozoa

Ramírez‐Ramírez

Salgado‐Lucio²,

Roa-Espitia³

et al. 2019

J of Cellular Biochemistry

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Actin cytoskeleton remodeling is a critical process for the acquisition of fertilizing capacity by spermatozoa during capacitation. However, the molecular mechanism that regulates this process has not been fully elucidated. In somatic cells, Ras‐related C3 botulinum toxin substrate 1 protein (Rac1) promotes the polymerization of actin by participating in the modeling of two structures: lamellipodia and adhesion complexes linked with the plasma membrane. Rac1 is expressed in mammalian spermatozoa; however, the role of Rac1 in sperm physiology is unknown. This study aimed to elucidate the participation of Rac1 in capacitation and acrosome reaction (AR). Rac1 was found to be dispersed throughout the acrosome and without changes in the middle piece. After 60 minutes of capacitation, Rac1 was found in the apical region of the acrosome only, which concurred with an increase in Rac1‐GTP. Rac1 inhibition prevented such changes. In the middle piece, Rac1 localization remained unchanged. Besides, Rac1 inhibition blocked capacitation and AR. The present study demonstrates that Rac1 participates only in the actin cytoskeleton remodeling that occurs in the acrosomal apical region during capacitation, a region where a large amount of actin is polymerized and shaped in a diadem‐like structure. Our data also show that this actin cytoskeleton organized by Rac1 interacts with filamin‐1, and such interaction was blocked by the inhibition of Rac1, which led to a different organization of the actin cytoskeleton. All these outcomes imply that the formation of an F‐actin cytoskeleton in the acrosomal apical region is a necessary event for capacitation and AR, and which is Rac1 driven.

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“…Sperm capacitation consists of a series of biochemical and biophysical changes in the sperm cells, which allow it to obtain the capacity to fertilize the oocyte. These changes occur in the head and tail of the sperm, triggering a cascade of signals at the molecular level (Abou‐Haila & Tulsiani, ; Gervasi & Visconti, ). As a result of capacitation, the sperm increases its motility and acquires the ability to experience the acrosome reaction (Florman, Jungnickel, & Sutton, ; Visconti et al, ; Visconti, Krapf, De La Vega‐beltrán, Acevedo, & Darszon, ).…”

Section: Introductionmentioning

confidence: 99%

Follicular fluid stimulates capacitation and acrosome reaction in alpaca sperm (Vicugna pacos)

Bravo

Valdivia

2018

Reprod Domestic Animals

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The follicular fluid exerts an effect on the sperm capacitation of several species; however, these effects vary according to species, both in the sperm motility and in the subsequent acrosome reaction. In this study, the effect of alpaca follicular fluid (aFF) on the motility and acrosome reaction of alpaca spermatozoa was observed, using follicular fluid of three follicle sizes: small (<3 mm), medium (3-6 mm) and large (>6 mm), in a concentration of 30%. Sperm motility at the first hour of incubation with aFF of small follicles was 48.0%, with aFF of medium follicles it was 43.33% and with aFF of large follicles, it was 34.53%, while control averaged 26.00%. At the second hour, control achieved an average of 28.13%, treatment with aFF from small follicles showed an average of 46.53%, with aFF from medium follicles it was 40.00% and with aFF from large follicles it was 35.60%. The acrosome reaction after 4 hours of incubation was 30.06% for control, whereas for aFF of small follicles it was 66.3%, with aFF of medium follicles it was 58.86% and for aFF of large follicles, it was 67.63%. In the case of sperm motility, a significant difference is demonstrated for all treatments in relation to the control at the first hour, whereas only the treatments with aFF of small and medium follicles show a significant difference with respect to the control at the second hour. In the case of the acrosome reaction, all treatments with follicular fluid show a significant difference with respect to the control. It was concluded that alpaca follicular fluid favours sperm capacitation and the acrosome reaction in alpaca spermatozoa.

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Chang's meaning of capacitation: A molecular perspective

Cited by 133 publications

References 173 publications

5’-AMP-Activated Protein Kinase Regulates Goat Sperm Functions via Energy Metabolism In Vitro

5’-AMP-Activated Protein Kinase Regulates Goat Sperm Functions via Energy Metabolism In Vitro

Rac1 is necessary for capacitation and acrosome reaction in guinea pig spermatozoa

Follicular fluid stimulates capacitation and acrosome reaction in alpaca sperm (Vicugna pacos)

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