The metabolite composition and antioxidant activity of the Saedanbaek, Jinyang, and Wooram soybean cultivars were evaluated. The moisture, crude ash, protein, fat, and carbohydrate contents in these soybean cultivars varied in the range 7.9-9.8 mg/g, 4.8-4.9 mg/g, 35.9-47.5 mg/g, 14.2-16.5 mg/g, and 21.4-35.0 mg/g, respectively. The crude protein content was found to be the highest 47.5 mg/g in the Saedanbaek soybean. The contents of saturated fatty acids such as palmitic acid, arachidic acid, behenic acid, and lignoceric acid were 1391.1, 490.8, 37.5, 44.9, and 16.6 mg/100 g, respectively, and were the highest in the Jinyang soybean. The unsaturated fatty acids (linoleic acid, oleic acid, and α-linolenic acid) were estimated to be 8258.0, 2896.8, and 906.5 mg/100 g, respectively, in the Saedanbaek, Jinyang, and Wooram soybeans. The highest total amino acid content (44598.54 mg/100 g) was found in the Saedanbaek soybean, and this was almost 2 and 1.6 folds higher than those in the Jinyang (21,525.93 mg/100 g) and Wooram (26,250.63 mg/100 g) soybeans. The highest glycosides, malonylglycosides, and aglycone contents (836.46 μg/g, 2,975.86 μg/g, and 231.60 μg/g, respectively) were found in the Wooram soybean.