Change in Plasma Total, Esterified and Non-esterified Capric Acid Concentrations during a Short-term Oral Administration of Synthetic Tricaprin in Dogs

Shrestha, Rojeet; Hirano, Ken‐ichi; Suzuki, Akira; Yamaguchi, Satoshi; Miura, Y.; Chen, Yifan; Mizuta, Masahiro; Chiba, Hitoshi; Hui, Shu‐Ping

doi:10.2116/analsci.33.1297

Cited by 9 publications

(11 citation statements)

References 50 publications

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“…Based upon these data, the Osaka University Hospital manufactured GMP-graded capsules containing the active gradients called CNT-01. We developed the assay to measure plasma capric acid levels [52, 53]. After finishing toxicity tests using rats and dogs required, we are finally conducting investigator-initiated clinical trials.…”

Section: Academia-initiated Development Of Specific Treatment For Tgcvmentioning

confidence: 99%

Triglyceride deposit cardiomyovasculopathy: a rare cardiovascular disorder

Hirano

Ikeda

et al. 2019

Orphanet J Rare Dis

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Triglyceride deposit cardiomyovasculopathy (TGCV) is a phenotype primarily reported in patients carrying genetic mutations in PNPLA2 encoding adipose triglyceride lipase (ATGL) which releases long chain fatty acid (LCFA) as a major energy source by the intracellular TG hydrolysis. These patients suffered from intractable heart failure requiring cardiac transplantation. Moreover, we identified TGCV patients without PNPLA2 mutations based on pathological and clinical studies . We provided the diagnostic criteria, in which TGCV with and without PNPLA2 mutations were designated as primary TGCV (P-TGCV) and idiopathic TGCV (I-TGCV), respectively. We hereby report clinical profiles of TGCV patients. Between 2014 and 2018, 7 P-TGCV and 18 I-TGCV Japanese patients have been registered in the International Registry. Patients with I-TGCV, of which etiologies and causes are not known yet, suffered from adult-onset severe heart disease, including heart failure and coronary artery disease, associated with a marked reduction in ATGL activity and myocardial washout rate of LCFA tracer, as similar to those with P-TGCV. The present first registry-based study showed that TGCV is an intractable, at least at the moment, and heterogeneous cardiovascular disorder.

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Section: Academia-initiated Development Of Specific Treatment For Tgcvmentioning

confidence: 99%

Triglyceride deposit cardiomyovasculopathy: a rare cardiovascular disorder

Hirano

Ikeda

et al. 2019

Orphanet J Rare Dis

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“…The serum sample preparation procedure was done in accordance to Shrestha et al as previously reported. 23 In brief, a portion of serum (25 lL for total FA, 100 lL for free FA) was spiked with the ISTD FA 11:0 (2 nmoL). For the total FA determination, saponification was performed by mixing the serum with 100 lL of 0.3 M KOH-EtOH and heating at 80 C for 45 min.…”

Section: Sample Preparationmentioning

confidence: 99%

“…And this method has been applied in our laboratory for determining medium-chain fatty acid (MCFA) and long-chain fatty acid (LCFA) of their total and free forms in various samples, ranging from the clinical blood sample to the commercial milk. [22][23][24] However, the strategy of HPLC with NPHderivatization was not applicable for SCFAs in our laboratory, due to the poor resolution and the interference in chromatography.…”

Section: Introductionmentioning

confidence: 98%

Determination of total, free and esterified short-chain fatty acid in human serum by liquid chromatography-mass spectrometry

Chen

Shrestha

et al. 2018

Ann Clin Biochem

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Background Short-chain fatty acids are primarily absorbed through the portal vein during lipid digestion, which is utilized as the energy source, as well as prevent type 2 diabetes and some cancers. However, reports on the determination of these short-chain fatty acids in human serum are limited. Methods Blood samples from human subjects ( n = 547, male/female = 246/301, age 58.85 ± 12.57) were collected. Saponification was applied to obtain total fatty acid. After derivatization by 2-nitrophenylhydrazine, fatty acid 4:0 and fatty acid 6:0 were measured by liquid chromatography-mass spectrometry. Results The developed method exhibited good linearity (R = 0.9996 for both). All the coefficients of variation of reproducibility and accuracy for fatty acid 4:0 and fatty acid 6:0 ranged 3.0%-6.1%, with the average recoveries of 87.8%-102.4% and 92.2%-98.2%, respectively. In all the samples, the concentration of fatty acid 4:0 (162.4 ± 76.4 μmol/L) was significantly higher than fatty acid 6:0 (2.0 ± 2.5 μmol/L, P < 0.001). Furthermore, the esterified form was predominant in both fatty acid 4:0 and fatty acid 6:0 (98.2% and 82.4% of total fatty acids, respectively). Besides, short-chain fatty acids showed no significant differences with regard to sex or age differences. Conclusion This developed liquid chromatography-mass spectrometry method is convenient and reliable, which might be useful for monitoring the variations of short-chain fatty acids in blood.

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“…First, the overall time for the preparation of samples has been significantly reduced in the present method. In our previously published report, 22 the pretreatment of samples using the conventional heating method takes 80 min, which can be shortened to 160 s using microwave heating. The derivatization alone needs 20 min of heating at 60 C in the conventional method, which can be decreased to 10 s using MAD.…”

Section: Comparison With Conventional Methodsmentioning

confidence: 99%

“…21 We have successfully applied the UV-HPLC method to monitoring the change of free and esterified capric acid (FA10:0) in serum samples after the oral administration of synthetic tricaprin. 22 The HPLC assay of FA hydrazides has enabled simple, sensitive, and specific measurements of FA.…”

Section: Introductionmentioning

confidence: 99%

Microwave-assisted Derivatization of Fatty Acids for Its Measurement in Milk Using High-Performance Liquid Chromatography

et al. 2018

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Fatty acid (FA) profiling of milk has important applications in human health and nutrition. Conventional methods for the saponification and derivatization of FA are time-consuming and laborious. We aimed to develop a simple, rapid, and economical method for the determination of FA in milk. We applied a beneficial approach of microwave-assisted saponification (MAS) of milk fats and microwave-assisted derivatization (MAD) of FA to its hydrazides, integrated with HPLC-based analysis. The optimal conditions for MAS and MAD were determined. Microwave irradiation significantly reduced the sample preparation time from 80 min in the conventional method to less than 3 min. We used three internal standards for the measurement of short-, medium- and long-chain FA. The proposed method showed satisfactory analytical sensitivity, recovery and reproducibility. There was a significant correlation in the milk FA concentrations between the proposed and conventional methods. Being quick, economic, and convenient, the proposed method for the milk FA measurement can be substitute for the convention method.

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Change in Plasma Total, Esterified and Non-esterified Capric Acid Concentrations during a Short-term Oral Administration of Synthetic Tricaprin in Dogs

Cited by 9 publications

References 50 publications

Triglyceride deposit cardiomyovasculopathy: a rare cardiovascular disorder

Triglyceride deposit cardiomyovasculopathy: a rare cardiovascular disorder

Determination of total, free and esterified short-chain fatty acid in human serum by liquid chromatography-mass spectrometry

Microwave-assisted Derivatization of Fatty Acids for Its Measurement in Milk Using High-Performance Liquid Chromatography

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