Change in redox state and heat shock protein expression in an Indian major carp <i>Cirrhinus cirrhosus</i> exposed to zinc and lead

Abstract

-Fish are exposed to different heavy metals that may induce numerous physiological changes. In the present study, we examined the redox state in response to a severe stress resulting from two heavy metals (Zinc and Lead) contamination in carp Cirrhinus cirrhosus. Fish were exposed to 1/10th of LC50 of the respective metals [zinc chloride (2.72 mg/L) and lead acetate (2.53 mg/L)] for 30 days and allowed to recover for another 30 days without any metal exposure. Concentration of metals, different enzymatic and non-enzymatic antioxidant agents and expression levels of heat shock protein (HSP) 70 and 90 were measured in the liver and the kidney of fish. The lipid peroxide levels in fish tissues gradually increased with duration of treatment for both metals. After 15 days of treatment, glutathione (GSH) levels had increased, but decreased as the treatment continued for 30 days and returned to basal levels after a 30-day recovery period. Activities of all the anti-oxidant enzymes, except glutathione peroxidase, in stressed fish were significantly increased compared to those in the control at 15 days and continued till the 30th day of treatment, showing a tendency to return to basal levels after the recovery period. Expression levels of HSP70 and HSP90 gradually increased after zinc and lead treatment, respectively. The expression of HSP was higher in the liver. The results suggest that different heavy metals may have differential effects on the redox state and induction of oxidative stress in carp, in vivo.

Early Developmental Stress Affects Subsequent Gene Expression Response to an Acute Stress in Atlantic Salmon: An Approach for Creating Robust Fish for Aquaculture?

“…The response of hsp 70 expression to stress differs depending on the timing, duration, type of stress and species (Takle et al 2005; e.g. , Moniruzzaman et al 2017). Repeated embryonic thermal stress has also been found to reduce or down-regulate subsequent hsp 70 responses to acute heat shock in whitefish (Whitehouse et al 2017).…”

Reference gene selection for quantitative gene expression analysis in black soldier fly (Hermetia illucens)

“…Researches showed that the expression of heat shock protein 90 ( Hsp90 ) in organisms will increase under heavy metal stress [34–36], so Hsp90 was selected as a target gene to evaluate the stability of candidate reference genes under different heavy metals. The normalization factors (NFs) were computed based on the geometric mean of genes with the lowest Geomean values as determined by RefFinder and a single reference gene with the lowest or highest Geomean value.…”

Integrated use of biomarkers to assess the impact of heavy metal pollution on Solea aegyptiaca fish in Lake Qarun

“…It prevents non-native proteins from aggregating and promotes their refolding and transportation to cellular organelles [142]. The significant increase in HSP70 levels in gills, liver, and muscles of Solea aegyptiaca fish obtained from the heavy metal polluted sites was observed by many investigators who reported increased expression of HSP70 due to metal exposure in different fish organs/ species such as Chanos chanos gills, liver and muscle [143], Tanichthys albonubes liver [144], Oreochromis niloticus muscle [145] and Cirrhinus cirrhosis liver [146]. In response to metal pollution stress, heat shock factors (HSFs) that are typically bind HSPs in the cytosol are dissociated from HSPs.…”