Change in salt tolerance of bread wheat as a result of the introgression of the genetic material of Aegilops speltoides and Triticum timopheevii

Yudina, R. S.; Леонова, И. Н.; Салина, Е. А.; Хлесткина, Е. К.

doi:10.1134/s2079059716030151

Cited by 9 publications

(6 citation statements)

References 18 publications

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“…Traits of interest include resistance to leaf rust including the genes Lr10 , Lr18 , and Lr50 ( McIntosh, 1983 ; Brown-Guedira et al, 2003 ; Leonova et al, 2010 ; Singh et al, 2017 ), stem rust including the genes Sr36 , Sr37 , and Sr40 ( Allard and Shands, 1954 ; McIntosh and Gyarfas, 1971 ; Dyck, 1992 ), powdery mildew including the genes Pm2 , Pm6, Pm27 , and Pm37 ( Allard and Shands, 1954 ; Jørgensen and Jensen, 1972 ; Peusha et al, 1995 ; Järve et al, 2000 ; Perugini et al, 2008 ; Qin et al, 2011 ), Fusarium head blight ( Malihipour et al, 2016 , 2017 ), black-point ( Lehmensiek et al, 2004 ), Hessian fly, Septoria blotch, wheat curl mite and tan spot ( Brown-Guedira et al, 1996 ). Triticum timopheevii has also been shown to be a source of resistance to abiotic stresses such as salt tolerance ( Badridze et al, 2009 ; Yudina et al, 2016 ) and traits affecting grain quality such as milling yield and grain protein ( Lehmensiek et al, 2008 ) and mineral content ( Hu et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Introgression of the Triticum timopheevii Genome Into Wheat Detected by Chromosome-Specific Kompetitive Allele Specific PCR Markers

et al. 2022

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Triticum timopheevii (2n = 28, AtAtGG) is a tetraploid wild relative species with great potential to increase the genetic diversity of hexaploid wheat Triticum aestivum (2n = 42, AABBDD) for various important agronomic traits. A breeding scheme that propagated advanced backcrossed populations of wheat-T. timopheevii introgression lines through further backcrossing and self-fertilisation resulted in the generation of 99 introgression lines (ILs) that carried 309 homozygous segments from the At and G subgenomes of T. timopheevii. These introgressions contained 89 and 74 unique segments from the At and G subgenomes, respectively. These overlapping segments covered 98.9% of the T. timopheevii genome that has now been introgressed into bread wheat cv. Paragon including the entirety of all T. timopheevii chromosomes via varying sized segments except for chromosomes 3At, 4G, and 6G. Homozygous ILs contained between one and eight of these introgressions with an average of three per introgression line. These homozygous introgressions were detected through the development of a set of 480 chromosome-specific Kompetitive allele specific PCR (KASP) markers that are well-distributed across the wheat genome. Of these, 149 were developed in this study based on single nucleotide polymorphisms (SNPs) discovered through whole genome sequencing of T. timopheevii. A majority of these KASP markers were also found to be T. timopheevii subgenome specific with 182 detecting At subgenome and 275 detecting G subgenome segments. These markers showed that 98% of the At segments had recombined with the A genome of wheat and 74% of the G genome segments had recombined with the B genome of wheat with the rest recombining with the D genome of wheat. These results were validated through multi-colour in situ hybridisation analysis. Together these homozygous wheat-T. timopheevii ILs and chromosome-specific KASP markers provide an invaluable resource to wheat breeders for trait discovery to combat biotic and abiotic stress factors affecting wheat production due to climate change.

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Section: Introductionmentioning

confidence: 99%

Introgression of the Triticum timopheevii Genome Into Wheat Detected by Chromosome-Specific Kompetitive Allele Specific PCR Markers

et al. 2022

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“…Traits of interest include resistance to leaf rust including the genes Lr10, Lr18 and Lr50 (Brown-Guedira et al, 2003; Leonova et al, 2010; McIntosh 1983; Singh et al, 2017), stem rust including the genes Sr36, Sr37 and Sr40 (Allard and Shands 1954; Dyck 1992; McIntosh and Gyarfas 1971), powdery mildew including the genes Pm2, Pm6, Pm27 and Pm37 (Allard and Shands 1954; Järve et al, 2000; Jørgensen and Jensen 1972; Perugini et al, 2008; Peusha et al, 1995; Qin et al, 2011), Fusarium head blight (Malihipour et al, 2016; Malihipour et al, 2017), black-point (Lehmensiek et al, 2004), Hessian fly, Septoria blotch, wheat curl mite and tan spot (Brown-Guedira et al, 1996). T. timopheevii has also been shown to be a source of resistance to abiotic stresses such as salt tolerance (Badridze et al, 2009; Yudina et al, 2016) and traits affecting grain quality such as milling yield and grain protein (Lehmensiek et al, 2006) and mineral content (Hu et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Introgression of the Triticum timopheevii genome into wheat detected by chromosome-specific KASP markers

King

Grewal

Othmeni

et al. 2022

Preprint

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Triticum timopheevii (2n=28, AtAtGG) is a tetraploid wild relative species with great potential to increase the genetic diversity of hexaploid wheat Triticum aestivum (2n=42, AABBDD) for various important agronomic traits. A breeding scheme that propagated advanced backcrossed populations of wheat-T. timopheevii introgression lines through further backcrossing and self-fertilisation resulted in the generation of 99 introgression lines (ILs) that carried 309 homozygous segments from the At and G subgenomes of T. timopheevii. These introgressions contained 89 and 74 unique segments from the At and G subgenomes, respectively. These overlapping segments covered 98.9% of the T. timopheevii genome that has now been introgressed into bread wheat cv. Paragon including the entirety of all T. timopheevii chromosomes via varying sized segments except for chromosomes 3At, 4G and 6G. Homozygous ILs contained between one and eight of these introgressions with an average of three per introgression line. These homozygous introgressions were detected through the development of a set of 480 chromosome-specific Kompetitive allele specific PCR (KASP) markers that are well-distributed across the wheat genome. Of these, 149 were developed in this study based on single nucleotide polymorphisms (SNPs) discovered through whole genome sequencing of T. timopheevii. A majority of these KASP markers were also found to be T. timopheevii subgenome specific with 182 detecting At subgenome and 275 detecting G subgenome segments. These markers showed that 98% of the At segments had recombined with the A genome of wheat and 74% of the G genome segments had recombined with the B genome of wheat with the rest recombining with the D genome of wheat. These results were validated through multi-colour in situ hybridisation analysis. Together these homozygous wheat-T. timopheevii ILs and chromosome-specific KASP markers provide an invaluable resource to wheat breeders for trait discovery to combat biotic and abiotic stress factors affecting wheat production due to climate change.

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“…T. timopheevii is actively involved in modern breeding programs to create introgression lines and increase the genetic diversity of modern wheat due to its partial homology with the common wheat genome (BBA u A u DD) [1,2]. T. timopheevii has been shown to be a valuable donor for resistance genes to wheat fungal diseases [3][4][5], environmental stresses [6], and modified protein content [7]. In addition to direct crosses between Timopheev's wheat and common wheat and the production of synthetic amphiploids, an acceleration of the wheat breeding process can be brought about by the application of the various biotechnological techniques.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Daminozide, Dark/Light Schedule and Copper Sulphate in Tissue Culture of Triticum timopheevii

Miroshnichenko

Klementyeva

Dolgov

2021

Plants

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Triticum timopheevii Zhuk. is a tetraploid wheat that is utilized worldwide as a valuable breeding source for wheat improvement. Gene-based biotechnologies can contribute to this field; however, T. timopheevii exhibits recalcitrance and albinism in tissue cultures, making this species of little use for manipulation through genetic engineering and genome editing. This study tested various approaches to increasing in vitro somatic embryogenesis and plant regeneration, while reducing the portion of albinos in cultures derived from immature embryos (IEs) of T. timopheevii. They included (i) adjusting the balance between 2,4-D and daminozide in callus induction medium; (ii) cultivation using various darkness/illumination schedules; and (iii) inclusion of additional concentrations of copper ions in the tissue culture medium. We achieved a 2.5-fold increase in somatic embryogenesis (up to 80%) when 50 mg L−1 daminozide was included in the callus induction medium together with 3 mg L−1 2,4-D. It was found that the dark cultivation for 20–30 days was superior in terms of achieving maximum culture efficiency; moreover, switching to light in under 2 weeks from culture initiation significantly increased the number of albino plants, suppressed somatic embryogenesis, and decreased the regeneration of green plants. Media containing higher levels of copper ions did not have a positive effect on the regeneration of green plants; contrarily, the elevated concentrations caused albinism in plantlets. The results and relevant conclusions of the present study might be valuable for establishing an improved protocol for the regeneration of green plants in tissue cultures of T. timopheevii.

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Change in salt tolerance of bread wheat as a result of the introgression of the genetic material of Aegilops speltoides and Triticum timopheevii

Cited by 9 publications

References 18 publications

Introgression of the Triticum timopheevii Genome Into Wheat Detected by Chromosome-Specific Kompetitive Allele Specific PCR Markers

Introgression of the Triticum timopheevii Genome Into Wheat Detected by Chromosome-Specific Kompetitive Allele Specific PCR Markers

Introgression of the Triticum timopheevii genome into wheat detected by chromosome-specific KASP markers

The Effect of Daminozide, Dark/Light Schedule and Copper Sulphate in Tissue Culture of Triticum timopheevii

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