Change in the adhesive properties of blastomeres during early cleavage stages in sea urchin embryo

Masui, Mizuko; Kominami, Taro

doi:10.1046/j.1440-169x.2001.00555.x

Cited by 11 publications

(16 citation statements)

References 48 publications

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“…The difference between the two values was statistically significant (P<0.001, t=8.86, n=177). The value of control blastomeres is well coincided those reported in previous reports (Sano, 1977, Masui andKominami, 2001). Large deviation among observed values is probably due to difference in the mobilities between descendants of mesoand macromeres (Masui and Kominami, 2001).…”

Section: Negative Cell Surface Charges In Control and Licl-treated Emsupporting

confidence: 91%

“…To our knowledge, this is the first report that shows that LiCl decreases the amount of negative cell surface charges in early embryonic cells. Previously, we reported that the amount of negative cell surface charges decreased from the 5-6th cleavage stage, concomitantly with the increase in the adhesiveness of blastomeres (Masui and Kominami, 2001). It is naturally expected that the decrease in the negative cell surface charge makes the close cell apposition possible or makes the cell surface more adhesive.…”

Section: Licl Strengthens Cell Contactmentioning

confidence: 88%

“…The amount of negative surface charges was measured after the methods of Ohshima (1975). The detailed condition for cell electrophoresis was described before (Masui and Kominami, 2001).…”

Section: Cell Electrophoresismentioning

confidence: 99%

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Body Plan of Sea Urchin Embryo: An Ancestral Type Animal

Akasaka

Shimada

2001

Zoological Science

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Section: Negative Cell Surface Charges In Control and Licl-treated Emsupporting

confidence: 91%

Section: Licl Strengthens Cell Contactmentioning

confidence: 88%

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Body Plan of Sea Urchin Embryo: An Ancestral Type Animal

Akasaka

Shimada

2001

Zoological Science

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“…Thus, unlike in sea urchin embryos [5], a decrease of the negative surface charge density was not observed in starfish embryos during the early 9 cleavages.…”

Section: Changes Of the Negative Surface Charge Density During Cleavamentioning

confidence: 56%

“…For example, blastomeres of mouse embryos become so tightly compacted during the latter half of the 8-cell stage that the contours of each blastomere are hardly discernible [1][2][3]. In sea urchin embryos, blastomeres begin to adhere to their neighbors from the 5th or 6th cleavage onward, and form a monolayered hollow blastula [4,5].…”

Section: Introductionmentioning

confidence: 99%

Cell adhesion and the negative cell surface charges in embryonic cells of the starfish Asterina pectinifera

Masui¹,

Takata²,

Kominami³

2002

Electrophoresis

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Spherical blastomeres of starfish embryos begin to adhere to neighboring blastomeres and to become columnar in shape from the 7(th) or 8(th) cleavage onward. Studying development of embryos in the presence of LiCl, we found that developmental changes in cell-cell contacts were accelerated by LiCl. In order to learn why LiCl increased the adhesiveness between blastomeres, the negative surface charge density was estimated by the method of cell electrophoresis. It turned out that the electrophoretic mobility (EPM) of all blastomeres isolated from LiCl-treated embryos before the 512-cell stage was remarkably decreased. At the mid-gastrula stage, however, when constituent cells were connected with each other more tightly, the EPM was significantly retarded irrespectively whether the cells had been isolated from control or from LiCl-treated embryos. From these results of cell electrophoresis we conclude that reduction of the negative surface charge density may be one of the important factors that enhance the adhesion of starfish embryonic cells.

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