Change in the Electrical Impedance Caused by Cornification of the Epithelial Cell Layer of the Vaginal Mucosa in the Rat

Koto, Masao; Miwa, Masao; Tsuji, Kiichiro; Okamoto, Michio; Adachi, Jiro

doi:10.1538/expanim1978.36.2_151

Cited by 7 publications

(14 citation statements)

References 7 publications

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“…4,5 There have been several reports of similar devices in animal eyes, but to date, none in human eyes. [8][9][10][11][12][13][14] The purpose of this study was to determine whether our device can assess the CR value in healthy adult eyes.…”

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confidence: 99%

In Vivo Measurement of Human Corneal Impedance Value

Fukuda

Sekiya²

2016

Cornea

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confidence: 99%

In Vivo Measurement of Human Corneal Impedance Value

Fukuda

Sekiya²

2016

Cornea

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“…Such resistance measurements take only seconds to obtain and are much less amenable to subjective interpretation on the part of the technical operator than the more conventional vaginal smear methodology. Unfortunately, the instrumentation previously described to make such resistance measurements in the rat (1,3,4) is complex and difficult to fabricate in the laboratory. Two instruments have been manufactured specifically for rat and made commercially available in ready-to-use forms [i.e., the MK-10A impedance checker (Muromachi Kikai, Tokyo, Japan) and the EC40 estrus cycle monitor (Fine Science Tools, Foster City, CA)].…”

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confidence: 99%

“…But they are relatively expensive. This may explain why these two instruments plus the primary publications describing their initial use (3,4) have essentially remained uncited in the biomedical literature. As yet, there is no simple and inexpensive form of such instrumentation readily available to the average laboratory for routine use with rats.…”

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An inexpensive meter to measure differences in electrical resistance in the rat vagina during the ovarian cycle

Ramos

Lee

Peuler

2001

Journal of Applied Physiology

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The inherent electrical resistance of the rat vaginal wall rises markedly near the beginning of estrus and then falls again to low levels for the remainder of the ovarian cycle. Accordingly, special instruments have been developed to measure such resistances (within seconds) on simply inserting a small probe fitted with a pair of recording electrodes into the vagina (i.e., the MK-10A impedance checker and the EC40 estrus cycle monitor). As described herein, these two instruments are far more convenient for monitoring individual cycles than more laborious methods in which vaginal smears are inspected for changes in numbers of cornified (C), nucleated (N), and leukocytic (L) cells. However, they are also expensive and their use has essentially remained uncited in the literature. Thus we sought to determine whether a simple, inexpensive electrical meter (with resistance-measuring capacity), as commonly used by professional electricians, would serve the same purpose. We chose a standard multifunctional meter (model 22-178, RadioShack) and attached leads to it fabricated from the internal wiring of a shielded audio cable (model 42-2387A, RadioShack), one male terminal of which was used as a vaginal probe. In rats from which vagina smears revealed cell numbers in the order of C > N > L (typical of early estrus) electrical resistances were high, 488 +/- 130 k Omega (18 rats). In rats from which vagina smears revealed all other possible cell distributions, electrical resistances (combined) were much lower (P < 0.05), 124 +/- 23 k Omega (32 rats). Thus readily accessible, inexpensive electrical meters may be useful in assessing the status of estrus in female rats, either to improve reproductive efficiencies and/or for other purposes involving experiments in which such information is desirable.

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“…estrous stage, and EIV is a method to decide the proestrous stage by making use of this property [14]. During the experimental period, EIV was measured in all rats daily, and the rats were sacrificed on the proestrus day in the last week of the feeding period.…”

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Effect of Garcinia cambogia Administration on Female Reproductive Organs in Rats

Kiyose¹,

Kubo²,

Saito³

2006

J. Clin. Biochem. Nutr.

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Summary We investigated the effect of administering Garcinia cambogia (GA), which contains (−)-hydroxycitric acid ((−)-HCA), to female rats, focusing especially on changes in sexual hormones and its safety in terms of histopathological changes. A diet containing a high level of (−)-HCA (154 mmol / kg diet) was given to seven-week-old female rats with matched estrous cycles for two or four weeks. There were no significant differences in any of the serum hormones tested (follicle-stimulating hormone, luteinizing hormone, estradiol and progesterone) between the control group and the GA group during the observation period. Also, there were no abnormal morphological findings in the follicle and corpus luteum, nor were there any significant differences in the ovarian 4,4-dimethyl-5α-cholesta 8,14,24-triene-3β-ol (follicular fluid meiosis-activating sterol; FF-MAS) and 4,4-dimethyl-5α-cholesta-8,24-diene-3β-ol (testis meiosis-activating sterol; T-MAS) concentrations between the control and the GA groups. On the other hand, the final body weight of the GA group was statistically lower than that of the control group. The weight of abdominal fat in the GA group was markedly lower than that in the control group even after two weeks of feeding. Therefore, although we had confirmed in our previous study that GA had a marked effect on sexual maturation in male rats, no such deleterious influence was observed in the female rats.

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Change in the Electrical Impedance Caused by Cornification of the Epithelial Cell Layer of the Vaginal Mucosa in the Rat

Cited by 7 publications

References 7 publications

In Vivo Measurement of Human Corneal Impedance Value

In Vivo Measurement of Human Corneal Impedance Value

An inexpensive meter to measure differences in electrical resistance in the rat vagina during the ovarian cycle

Effect of Garcinia cambogia Administration on Female Reproductive Organs in Rats

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