Change in the expression of collagen genes in dividing and nondividing chondrocytes

Gauss, Verena; Müller, Peter

doi:10.1016/0005-2787(81)90206-9

Cited by 15 publications

(5 citation statements)

References 17 publications

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“…4 and 6) and confirmed by CNBr peptide analysis (not shown), occurred earlier in culture and constituted a higher proportion of total collagen synthesis in cultures on plastic than in cultures of collagen gels. The switch to type I collagen synthesis in both culture systems, however, was much less pronounced than has been previously observed (3,15,28) . This may be due to the relatively high initial plating densities used (lower plating densities have been reported to result in a more rapid switch to type I collagen synthesis [28]), the use of an efficiently buffered medium (30) or the use of fibronectin-depleted serum (32,40).…”

Section: Collagen Types Deposited In the Cell Matrixcontrasting

confidence: 53%

Effects of matrix macromolecules on chondrocyte gene expression: synthesis of a low molecular weight collagen species by cells cultured within collagen gels.

Gibson¹,

Schor²,

Grant³

1982

The Journal of Cell Biology

217

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Chick-embryo sternal chondrocytes have been cultured within three-dimensional Collagen gels as part of a study concerned with the effects of extracellular matrix macromolecules on chondrocyte gene expression . Data are presented indicating that chondrocytes cultured within such a collagenous environment synthesize significantly more of an hitherto unidentified, low molecular weight collagen species than do cells grown on plastic tissueculture dishes in the conventional manner . This low molecular weight collagen species contains noncollagenous domains (as indicated by its decreased molecular size after mild pepsin digestion), is distinct from the known collagen types (as judged by CNBr peptide analysis), and forms part of the insoluble collagenous matrix produced by the chondrocytes . Cells growing within the gel tend to form colonies consisting of a linear array of cells reminiscent of the cellular organization in growth cartilage.Although chondrocytes explanted to plastic tissue-culture dishes initially synthesize cartilage-specific macromolecules, it has proved difficult to maintain the expression of a differentiated phenotype during subsequent culture in vitro . The common observation is that chondrocytes in vitro tend to assume a fibroblastoid morphology with a concomitant switch from the predominant synthesis of type II to type I collagen (26).A number of culture parameters have been observed to affect chondrocyte behaviour in vitro . For example, Von der Mark et al. (39) have demonstrated that cell density and position within a colony influence the ability of chondrocytes to synthesize a cartilage-specific matrix. Cell-produced matrix macromolecules may play an important role in this regulatory process since the presence of exogenous collagen (23), proteoglycan (17, 18) and fibronectin (32, 40) have all been observed to influence the quantity of cartilage-specific components synthesized by chondrocytes in vitro . Indeed, the differentiation of a number of cell types including epidermal basal cells (29), hepatocytes (34), mammary epithelial cells (41) and fibroblasts (14) has been shown to be affected by culture on a collagen substratum.The present study was initiated to investigate further the effects of a collagen substratum on the synthesis of different

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Section: Collagen Types Deposited In the Cell Matrixcontrasting

confidence: 53%

Effects of matrix macromolecules on chondrocyte gene expression: synthesis of a low molecular weight collagen species by cells cultured within collagen gels.

Gibson¹,

Schor²,

Grant³

1982

The Journal of Cell Biology

217

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“…Rat fetal fibroblasts in culture released predominantly rat IGF II and only small amounts of rat IGF I (25). However, postnatal cultured rat fibroblasts released increasing amounts of rat IGF I and decreasing amounts of rat IGF II in an age-dependent manner (25 (27).…”

Section: Discussionmentioning

confidence: 99%

Human fetal and adult chondrocytes: effect of insulin-like growth factors I and II, insulin and growth hormone on clonal growth

Vetter¹,

Zapf

Heit

et al. 1986

Acta Endocrinologica

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“…A dedifferentiating effect on cultured chondrocytes has also been described when using several other growth promoting agents, including the thymidine analog 5-BrDU, embryo extracts (Mayne et al, 1976), or serum (Gauss et al, 1981 (Froger-Gaillard et al, 1989). It has been proposed by Zezulak and Green (1986) …”

mentioning

confidence: 98%

Modulation of sulfated proteoglycan synthesis and collagen gene expression by chondrocytes grown in the presence of bFGF alone or combined with IGF₁

Nataf

Tsagris²,

Dumontier³

et al. 1990

Reprod. Nutr. Dévelop.

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Change in the expression of collagen genes in dividing and nondividing chondrocytes

Cited by 15 publications

References 17 publications

Effects of matrix macromolecules on chondrocyte gene expression: synthesis of a low molecular weight collagen species by cells cultured within collagen gels.

Effects of matrix macromolecules on chondrocyte gene expression: synthesis of a low molecular weight collagen species by cells cultured within collagen gels.

Human fetal and adult chondrocytes: effect of insulin-like growth factors I and II, insulin and growth hormone on clonal growth

Modulation of sulfated proteoglycan synthesis and collagen gene expression by chondrocytes grown in the presence of bFGF alone or combined with IGF₁

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Change in the expression of collagen genes in dividing and nondividing chondrocytes

Cited by 15 publications

References 17 publications

Effects of matrix macromolecules on chondrocyte gene expression: synthesis of a low molecular weight collagen species by cells cultured within collagen gels.

Effects of matrix macromolecules on chondrocyte gene expression: synthesis of a low molecular weight collagen species by cells cultured within collagen gels.

Human fetal and adult chondrocytes: effect of insulin-like growth factors I and II, insulin and growth hormone on clonal growth

Modulation of sulfated proteoglycan synthesis and collagen gene expression by chondrocytes grown in the presence of bFGF alone or combined with IGF1

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Modulation of sulfated proteoglycan synthesis and collagen gene expression by chondrocytes grown in the presence of bFGF alone or combined with IGF₁