Change of drug resistance patterns and genetic properties of R plasmids in<i>Salmonella typhimurium</i>of bovine origin isolated from 1970 to 1979 in northern Japan

Makino, Sou‐ichi; Ishiguro, Naotaka; Sato, Gihei; Seno, Noboru

doi:10.1017/s0022172400069473

Cited by 10 publications

(7 citation statements)

References 18 publications

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“…This study shows that the incidence of plasmids, drug resistance, and conjugative R plasmids was extraordinarily high in S. typhimurium strains isolated in Japan. Similar data on Salmonella isolates including S. typhimurium have been published elsewhere (8,22). We isolated S. typhimurium strains from apparently healthy male calves and found a high incidence of multiple drug resistance and plasmids of various sizes (10).…”

Section: Discussionsupporting

confidence: 77%

Plasmid profile analysis in epidemiological studies of animal Salmonella typhimurium infection in Japan

Nakamura¹,

Sato²,

Ohya³

et al. 1986

J Clin Microbiol

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Plasmid profiles were investigated in 65 isolates of SalmoneUla typhimurium derived from aninal outbreaks during the period of 1978 through 1983 in Japan. Incidence of plasmids, drug-resistance, and conjugative R plasmids were extraordinarily high in these isolates. This high incidence reflects the prophylactic and therapeutic use of antibiotics. Most isolates from diseased animals, cohabiting animals, and each farm showed the same or similar plasmid patterns. However, there was a difference in plasmid patterns within strains isolated from each of several animals. It may be that one or two plasmids were introduced or deleted in these strains, leading to the difference discerned in strains isolated from the same animal. It was also shown that during one epidemic, two strains of S. typhimurium were involved that could be distinguished by plasmid profile analysis. Our conclusion is that when S. typhimurium strains isolated from animals reared in limited areas exhibit identical or similar plasmid patterns, they are derived from the same source and that when strains isolated in a limited area exhibit quite a different plasmid pattern, these strains are derived from independent sources.

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Section: Discussionsupporting

confidence: 77%

Plasmid profile analysis in epidemiological studies of animal Salmonella typhimurium infection in Japan

Nakamura¹,

Sato²,

Ohya³

et al. 1986

J Clin Microbiol

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“…To clarify whether or not S. Typhimurium isolates have the ability to transfer their resistance genes, liquid and filter mating procedures were performed in vitro (21) using rifampicin-resistant Escherichia coli C600 (Rif r ) and Nal-resistant S. Dublin (Nal r ) derivatives as recipient strains. S. Typhimurium isolate No.7 was used as the donor strain.…”

Section: Conjugate Experiments In Vitromentioning

confidence: 99%

<i>Salmonella</i> Typhimurium Isolated from Healthy Pigs and Their Ability of Horizontal Transfer of Multidrug Resistance and Virulence Genes

Thủy

Takeshi

Kusumoto

et al. 2009

Bioscience Microflora

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Salmonella is the most common food-borne pathogen worldwide. Rapid dissemination of multidrug-resistant strains, in particular from animal origins, is a growing concern to human and animal health, and it is mostly attributed to conjugative DNA exchange in the intestinal tract of food animals. To understand the potential role of pigs as a reservoir for antimicrobial-resistant Salmonella, we isolated Salmonella from slaughtered pigs and examined their drug resistance and gene transfer ability. We collected fecal and carcass swabs from 104 healthy pigs at an abattoir in Obihiro, Hokkaido, Japan. A total of 15 Salmonella strains were isolated, the most common serotypes being S. Typhimurium (7/15), S. Derby (2/15), S. Southampton (2/15) and S. O4:d:-(4/15). All of S. Typhimurium isolates were resistant to at least one of five antibiotics (ampicillin, kanamycin, sulfisoxazole, tetracycline and streptomycin). Pulsedfield gel electrophoresis (PFGE) profiles after XbaI and BlnI digestion were analyzed. S. Typhimurium isolates from 3 farms located in different regions clustered together and showed genetic relatedness. In conjugation experiments, one multidrug-resistant S. Typhimurium isolate showed the ability to transfer not only antibiotic resistance genes but also virulence genes such as spvABC to recipient bacteria. These results suggest that the spread of S. Typhimurium had occured in pig farms and that asymptomatic Salmonella-infected pigs should be considered as a significant source of antibiotic-resistant bacteria.

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“…Large plasmid DNA was isolated and agarose gel electrophoresis was performed as previously described [4]. The R plasmids were detected by filter mating at 37 mC using E. coli C600 Rif r as the recipient strain and Tet r O157 isolates as the donor strains as previously described [5]. DNA sequencing was performed using an ABI PRISM 377 (Applied Biosystems).…”

Section: Other Techniquesmentioning

confidence: 99%

Molecular epidemiological study on tetracycline resistance R plasmids in enterohaemorrhagic Escherichia coli O157[ratio ]H7

et al. 1999

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Restriction patterns obtained with EcoRI and Southern hybridization were used for the differentiation of tetracycline-resistant (Tet(r)) R plasmids in enterobaemorrhagic Escherichia coli (EHEC) O157:H7 isolates from a mass outbreak at a kindergarten in Obihiro-City, Hokkaido, Japan, 1996. Two kinds of Tet(r) R plasmids of 50 and 95 kb were detected. The 50-kb plasmids were identical to each other, while the 93-kb plasmids were of three types that were very similar to each other. The tet genes of both 50- and 95-kb R plasmids were 100% identical to the tet gene of pSC101 and all plasmids hybridized to a probe for tet. Because food-origin O157 strains were sensitive to tetracycline, we concluded that such Tet(r) R-plasmids might transfer to drug-sensitive O157 strains in the infected individuals.

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Change of drug resistance patterns and genetic properties of R plasmids inSalmonella typhimuriumof bovine origin isolated from 1970 to 1979 in northern Japan

Cited by 10 publications

References 18 publications

Plasmid profile analysis in epidemiological studies of animal Salmonella typhimurium infection in Japan

Plasmid profile analysis in epidemiological studies of animal Salmonella typhimurium infection in Japan

<i>Salmonella</i> Typhimurium Isolated from Healthy Pigs and Their Ability of Horizontal Transfer of Multidrug Resistance and Virulence Genes

Molecular epidemiological study on tetracycline resistance R plasmids in enterohaemorrhagic Escherichia coli O157[ratio ]H7

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