Change of supercooling capability in solutions containing different kinds of ice nucleators by flavonol glycosides from deep supercooling xylem parenchyma cells in trees

Kuwabara, Chikako; Kasuga, Jun; Wang, Donghui; Fukushi, Yukiharu; Arakawa, Keita; Koyama, Toshie; Inada, Takaaki; Fujikawa, Seizo

doi:10.1016/j.cryobiol.2011.06.001

Cited by 14 publications

(10 citation statements)

References 21 publications

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“…Although the seasonal changes in these polyphenol contents have not been determined, it is possible that these substances are involved in the mechanism of deep supercooling as facilitators or stabilizers of the supercooling state. Our results also suggested that the anti‐ice nucleation activity of these substances changed greatly depending on the kind of ice nucleator in solutions and quantitative ratio between anti‐ice nucleation substances and ice nucleators (Kuwabara et al , Wang et al ). From these results, we speculate that quantitative and qualitative changes in both ice nucleators and anti‐ice nucleation substances and their interaction might determine the seasonal changes in supercooling capability of XPCs.…”

Section: Discussionsupporting

confidence: 58%

Roles of cell walls and intracellular contents in supercooling capability of xylem parenchyma cells of boreal trees

Kasuga

Endoh

Yoshiba

et al. 2012

Physiologia Plantarum

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The supercooling capability of xylem parenchyma cells (XPCs) in boreal hardwood species differs depending not only on species, but also season. In this study, the roles of cell walls and intracellular contents in supercooling capability of XPCs were examined in three boreal hardwood species, Japanese beech, katsura tree and mulberry, whose supercooling capability differs largely depending on species and season. XPCs in these species harvested in winter and summer were treated by rapid freezing and thawing (RFT samples) or by RFT with further washing (RFTW samples) to remove intracellular contents from XPCs in order to examine the roles of cell walls in supercooling. RFT samples were also treated with glucose solution (RFTG samples) to examine roles of intracellular contents in supercooling. The supercooling capabilities of these samples were examined by differential thermal analysis after ultrastructural observation of XPCs by a cryo-scanning electron microscope to confirm effects of the above treatments. XPCs in RFTW samples showed a large reduction in supercooling capability to similar temperatures regardless of species or season. On the other hand, XPCs in RFTG samples showed a large increase in supercooling capability to similar temperatures regardless of species or season. These results indicate that although cell walls have an important role in maintenance of supercooling, change in supercooling capability of XPCs is induced by change in intracellular contents, but not by change in cell wall properties.

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Section: Discussionsupporting

confidence: 58%

Roles of cell walls and intracellular contents in supercooling capability of xylem parenchyma cells of boreal trees

Kasuga

Endoh

Yoshiba

et al. 2012

Physiologia Plantarum

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“…The droplet freezing assay has shown that anti-INAs of kaempferol 7-O-β-glucoside (K7G) and (-)-epigallocatechin gallate (EGCG) were 5.7 and 3.3°C, respectively, in the previous study 11,12) . They were used as positive controls for the examination of leaf disk freezing assay system.…”

Section: Examination Of Leaf Disk Freezing Assay For Anti-ice Nucleatmentioning

confidence: 92%

“…The freezing temperature of the solution was measured by a droplet freezing assay, as described in our previous report 12) , which was slightly modified from the assay developed by Vali 18) . Two μL aliquots of the sample solution containing 0.10 g FW/mL leaf extract, 2.0 mg/mL ice-nucleating bacteria, and 50 mM potassium phosphate buffer (pH 7.0) were loaded on the copper plate coated with mineral oil (Nacalai Tesque; Kyoto, Japan).…”

Section: Droplet Freezing Assaymentioning

confidence: 99%

“…The intensities of the anti-INA have been reported to depend on the flavonoid types, and several flavonoids have been found to demonstrate sufficient activity, which decrease the freezing temperature over 2°C, in solutions containing E. ananas. Anti-INA may be influenced by both aglycone types and modifications in their structures 9,12) . Plants accumulate various flavonoids and their glycosides as secondary metabolites.…”

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confidence: 99%

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Screening of plant resources with anti-ice nucleation activity for frost damage prevention

Suzuki

Fukuda

Fukushi

et al. 2017

Bioscience, Biotechnology, and Biochemistry

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Previous studies have shown that some polyphenols have anti-ice nucleation activity (anti-INA) against ice-nucleating bacteria that contribute to frost damage. In the present study, leaf disk freezing assay, a test of in vitro application to plant leaves, was performed for the screening of anti-INA, which inhibits the ice nucleation activity of an ice-nucleating bacterium Erwinia ananas in water droplets on the leaf surfaces. The application of polyphenols with anti-INA, kaempferol 7-O-β-glucoside and (-)-epigallocatechin gallate, to the leaf disk freezing assay by cooling at -4--6 °C for 3 h, revealed that both the compounds showed anti-INAs against E. ananas in water droplets on the leaf surfaces. Further, this assay also revealed that the extracts of five plant leaves showed high anti-INA against E. ananas in water droplets on leaf surfaces, indicating that they are the candidate resources to protect crops from frost damage.

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“…Perennial plants that persist in colder climates can acclimate to tolerate extracellular ice formation. Some perennial plants exhibit mixed modes of protection with most cells undergoing equilibrium extracellular freezing, but with groups of cells or organs deep supercooling (Kuwabara et al, 2011).…”

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confidence: 99%

Does FreezePruf Topical Spray Increase Plant Resistance to Freezing Stress?

Anderson¹

2012

hortte

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One method of plant freeze protection involves the application of compounds that promote freeze avoidance or tolerance. FreezePruf, a commercially available product recently marketed to improve both freeze avoidance and tolerance, contains polyethylene glycol, potassium silicate, glycerol, silicone polyether surfactant, and a bicyclic oxazolidine antidessicant. The goal of the present study was to evaluate the protection level provided by FreezePruf using laboratory-based methods involving plants and plant parts from species capable and incapable of lowtemperature acclimation. FreezePruf did not lower the freezing temperature of pepper (Capsicum annuum) seedlings, celosia (Celosia argentea) seedlings, detached tomato (Solanum lycopersicum) leaves, or postharvest tomato fruit. Spray application of the putative cryoprotectant did not increase the freeze tolerance of bermudagrass (Cynodon dactylon) crowns or stolons. It is possible that a greater level of protection could be achieved with other species or different experimental protocols.

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Change of supercooling capability in solutions containing different kinds of ice nucleators by flavonol glycosides from deep supercooling xylem parenchyma cells in trees

Cited by 14 publications

References 21 publications

Roles of cell walls and intracellular contents in supercooling capability of xylem parenchyma cells of boreal trees

Roles of cell walls and intracellular contents in supercooling capability of xylem parenchyma cells of boreal trees

Screening of plant resources with anti-ice nucleation activity for frost damage prevention

Does FreezePruf Topical Spray Increase Plant Resistance to Freezing Stress?

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