Change of Th0 to Th1 Cell‐Cytokine Profile Following Tuberculosis Chemotherapy

Dieli, Francesco; Singh, Melissa; Spallek, Ralf; Romano, Amelia; Titone, Lucina; Sireci, Guido; Friscia, Giuseppe; Sano, Caterina Di; Santini, Daniele; Salerno, Alfredo; Iványi, Juraj

doi:10.1046/j.1365-3083.2000.00744.x

Cited by 29 publications

(33 citation statements)

References 19 publications

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“…This observation is consistent with previous studies, namely that this chemotherapy in TB patients leads to an increase of IFNg production specific for different antigenic peptides. 31,32 Interestingly, mice immunized with empty plasmid DNA during chemotherapy course showed a higher level of IFN-g production in response to both CF and Ag85A protein than the control mice given chemotherapy alone (Figures 3a and b), suggesting that the CpG motifs in DNA vector could work as an inducer of Th1 immune response, as previously reported. 33 Expectedly, mice immunized with IL-12N220L DNA showed a higher level of IFN-g production in response to CF than mice immunized with empty plasmid DNA or Ag85A DNA (Figure 3a, Po0.03 in both cases).…”

Section: Establishment Of a Latent Infection Modelsupporting

confidence: 76%

Therapeutic effect of DNA vaccines combined with chemotherapy in a latent infection model after aerosol infection of mice with Mycobacterium tuberculosis

Jeon

Kim

et al. 2003

Gene Ther

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Section: Establishment Of a Latent Infection Modelsupporting

confidence: 76%

Therapeutic effect of DNA vaccines combined with chemotherapy in a latent infection model after aerosol infection of mice with Mycobacterium tuberculosis

Jeon

Kim

et al. 2003

Gene Ther

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“…Moreover, strong T-cell responses to HspX in African populations were observed that were mostly restricted to latently infected individuals (14). In addition, T cells from TB patients recognizing the HspX protein showed a switch from Th0 toward Th1 after chemotherapy, indicating their potential to induce protective T-cell responses (9,15). These characteristics of HspX make it an interesting target for postexposure TB vaccination, as well as for the possible diagnosis of preclinical infection.…”

Section: Discussionmentioning

confidence: 99%

T-Cell Recognition of the HspX Protein ofMycobacterium tuberculosisCorrelates with LatentM. tuberculosisInfection but Not withM. bovisBCG Vaccination

et al. 2007

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Tuberculosis (TB) is one of the leading causes of mortality (two million annually) from an infectious disease, particularly in the developing world (36). It is estimated that 2 billion people are latently infected with Mycobacterium tuberculosis, which causes active disease in 5 to 10% of infected individuals. Most individuals initially control the infection by mounting cell-mediated immunity. However, residual mycobacteria remain viable for many years in healthy immunocompetent hosts in the absence of disease (4), and the majority of contagious TB disease cases will arise from this enormous source of latent TB infection.The currently available vaccine against TB, M. bovis BCG, is largely ineffective at protecting against pulmonary disease in adults (16,23). The precise nature of the T-cell response needed for protection against adult pulmonary TB is incompletely defined, as is the cause for the lack of protection by BCG vaccination (3).The 16-kDa heat shock protein HspX (Rv2031c) is required for mycobacterial persistence within the macrophage and is a dominant protein produced during static growth or under oxygen deprivation (37). Under these conditions, it can account for up to 25% of the total bacillary protein expression. It is proposed that HspX plays an active role in slowing the growth of M. tuberculosis in vivo immediately after infection, as M. tuberculosis mutants lacking the hspX gene exhibited increased growth both in mice and in macrophages (24). In addition to the presence of specific humoral responses against HspX in the sera of cavitary TB patients (29), both T-cell and B-cell responses to HspX were found to be associated with latent M. tuberculosis infection (13, 14), pointing to the importance of HspX as an antigenic target of immune responses during latent TB infection.Since new vaccines containing relevant fragments of HspX, may induce improved responses against this TB latency antigen, we have generated and characterized HspX-specific, human CD8 ϩ and CD4 ϩ T cells, restricted by common human lymphocyte antigen (HLA) class I and class II alleles. In addition, peripheral blood mononuclear cells (PBMC) from M. tuberculosis-infected individuals (both active and latent infections) and BCG-vaccinated individuals with or without exposure to M. tuberculosis were examined for their in vitro response to HspX. Finally, the effect of BCG-or HspX immunization on induced immunity against HspX was analyzed in HLA-A2/K b and HLA-DR3.Ab 0 transgenic (tg) mice. MATERIALS AND METHODSAntigens. BCG (M. bovis bacillus Calmette-Guérin, Danish 1331) was purchased from the Statens Serum Institute (Copenhagen, Denmark), and killed M. tuberculosis H37Rv sonicate was obtained from D. van Soolingen (RIVM, The Netherlands). The antigen 85B gene (Rv1886c) and the hspX gene (Rv2031c) of M. tuberculosis were amplified by PCR and cloned by Gateway Technology (Invitrogen, San Diego, CA) in a bacterial expression vector containing an

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“…In association with host defenses against tuberculosis, activation of T helper (Th)1 cells and production of Th1-associated cytokines, such as IFN-c and interleukin-12 (IL-12), are critical [13]. However, recent reports suggest that Th2 activation may also be elicited by tuberculosis, resulting in increased IgE production [14].…”

Section: Discussionmentioning

confidence: 99%

A case of immunoglobulin G4-related chronic sclerosing sialadenitis and dacryoadenitis associated with tuberculosis

et al. 2008

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We describe a 64-year-old woman with chronic sclerosing sialadenitis and dacryoadenitis, which developed during treatment for cervical lymph node tuberculosis. Anti-tuberculosis treatment did not improve the swelling in the lacrimal and submandibular glands, and a biopsy specimen of the lacrimal gland showed inflammation, with abundant lymphoid follicles with fibrosis and granuloma without caseous necrosis. Immunohistological examination of a repeat biopsy specimen showed abundant immunoglobulin (Ig) G4-positive plasma cell infiltration. Corticosteroid therapy improved the salivary gland swelling without reactivation of the tuberculosis. This case suggests that an abnormal immunological reaction to tuberculosis may be one of the etiological candidates for IgG4-related disease.

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Change of Th0 to Th1 Cell‐Cytokine Profile Following Tuberculosis Chemotherapy

Cited by 29 publications

References 19 publications

Therapeutic effect of DNA vaccines combined with chemotherapy in a latent infection model after aerosol infection of mice with Mycobacterium tuberculosis

Therapeutic effect of DNA vaccines combined with chemotherapy in a latent infection model after aerosol infection of mice with Mycobacterium tuberculosis

T-Cell Recognition of the HspX Protein ofMycobacterium tuberculosisCorrelates with LatentM. tuberculosisInfection but Not withM. bovisBCG Vaccination

A case of immunoglobulin G4-related chronic sclerosing sialadenitis and dacryoadenitis associated with tuberculosis

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