Changes and clinical significance of peripheral blood helper T lymphocyte and natural killer (NK) cells in unexplained recurrent spontaneous abortion (URSA) patients after abortion and successful pregnancy

Ly, Zhu; Chen, X; Xu, Z.; Xu, Lei; Mao, Tianyi; Zhang, H

doi:10.12891/ceog1789.2015

Cited by 16 publications

(19 citation statements)

References 20 publications

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“…From the 97 records sought for retrieval, 94 full-text articles were obtained and critically analysed, leading to the exclusion of 75 studies due to ineligibility-related reasons. Finally, a total of 19 studies [ 17 , 19 , 20 , 30 , 31 , 32 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 ] were included in the qualitative analysis and 8 [ 20 , 32 , 42 , 43 , 46 , 48 , 50 , 54 ] were included in the quantitative analysis. Eleven studies were excluded from the meta-analysis because one had a high risk of bias [ 47 ] and ten [ 17 , 19 , 30 , 31 , 44 , 45 , 49 , 51 , 52 , 53 ] did not have the data required for performing the analysis.…”

Section: Resultsmentioning

confidence: 99%

Association of B Cells with Idiopathic Recurrent Pregnancy Loss: A Systematic Review and Meta-Analysis

Ângelo-Dias

Martins

Dias

et al. 2022

IJMS

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Recurrent pregnancy loss (RPL) affects 1–2% of women and is defined as having experienced two or more failed pregnancies. In almost 50% of cases, the causes are idiopathic (IRPL), but increasing evidence has suggested an immunological cause. B cells are known to provide crucial support for a successful pregnancy outcome. However, their involvement in the mechanisms underlying IRPL is still unclear. This systematic review and meta-analysis aimed to comprehensively summarise the existing evidence regarding the levels and profiles of B cells in IRPL. An extensive computerized search in PubMed/Medline, Embase, Scopus, and Web of Science databases was performed with no imposed limits. Two reviewers independently screened all retrieved studies, extracted all the data, and assessed the methodological quality. Disagreements were resolved by a third reviewer. From a total of 1125 retrieved studies, 19 studies were included in the systematic review, and 8 studies were quantitatively analysed. We highlight a potential association between women with IRPL and increased levels of endometrial B cells. In addition, the flow cytometry technique seems to be preferred over immunohistochemistry for identifying those differences, while further studies are necessary to clarify the role of B cells as an immunological risk factor for RPL.

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Section: Resultsmentioning

confidence: 99%

Association of B Cells with Idiopathic Recurrent Pregnancy Loss: A Systematic Review and Meta-Analysis

Ângelo-Dias

Martins

Dias

et al. 2022

IJMS

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“…Among 26 included women, 16 women had recurrent implantation failures, with median age of 37 years (29-43) and 9.5 (3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)) embryo transfers (Table 1 and Supplementary Table 2).…”

Section: Resultsmentioning

confidence: 99%

“…Immune abnormalities have been reported in women with RM and RIF, such as increased blood natural killer (NK) cell levels, regulatory T cell deficiencies and increased proinflammatory cytokines 3 . Uterine NK cells (uNK) represent up to 70% of the cells in endometrium during the implantation phase and secrete several cytokines (IL-12, IL-15, Fn-14, IL-18, LIF, TWEAK) modulating the uterine environment.…”

Section: Introductionmentioning

confidence: 99%

Intralipid therapy for unexplained recurrent miscarriage and implantation failure: Case-series and literature review

Plaçais

Kolańska

Kraiem

et al. 2020

European Journal of Obstetrics & Gynecology and Reproductiv

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“…Many famous doctors practicing Chinese medicine have treated RSA and threatened abortion with variants of STW and achieved good clinical efficacy [ 2 ]. In recent years, research on the mechanism of STW by scholars of Chinese medicine has been deepening [ 3 ], but the specific molecular mechanism by which STW prevents miscarriage needs further exploration.…”

Section: Introductionmentioning

confidence: 99%

“…Relatively definite pathogenic factors mainly include chromosomes, endocrine, infection, anatomical abnormalities of internal reproductive organs, and immunity [ 4 ]. However, the etiology remains unclear in more than 40% of clinical patients [ 3 ]. Important causes of pregnancy loss are impaired trophoblast function (migration and invasion) and embryo implantation failure [ 5 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

Shoutai Wan Improves Embryo Survival by Regulating Aerobic Glycolysis of Trophoblast Cells in a Mouse Model of Recurrent Spontaneous Abortion

Liang

Tang

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Background. During embryo implantation, the blastocyst exhibits a high capacity for aerobic glycolysis, which results in a unique microenvironment of high lactate/low pH at the maternal-fetal interface. Shoutai Wan (STW) is an effective Chinese herbal formula widely used in the clinical treatment of recurrent spontaneous abortion (RSA). However, the specific molecular mechanism by which STW prevents abortion is yet to be elucidated. Methods. Female CBA/J mice were allocated into six groups randomly and then mated with BALB/c mice as the control group, DBA/2 mice as the RSA model, CBA/J×DBA/2 mice treated with dydrogesterone as the DQYT group, or CBA/J×DBA/2 mice treated with low, medium, and high-dose STW as the STW-L, STW-M, and STW-H groups, respectively. Drug administration started 14 days before mating and ended on the 14th day of pregnancy. The embryo loss rate of each group was calculated on day 14 of gestation, and the pregnancy outcomes of the mice in each group were observed. The mouse serum was collected to determine the levels of progesterone (P) and chorionic gonadotropin (CG). The activities of HK2, PKM2, and LDHA, the key glycolytic enzymes in each group, were detected. The expressions of lactate, ATP, HK2, PKM2, LDHA, MCT4, GLUT1, and GPR81 as well as the morphology of trophoblast cells were examined. Results. The embryo loss rate and adverse pregnancy outcomes were significantly increased ( P < 0.05 ) in the RSA model group. After dydrogesterone or different doses of STW treatment, the embryo loss rate and adverse pregnancy outcomes were rescued to varying degrees ( P < 0.05 ). Interestingly, there was no significant difference among the groups in terms of serum P and CG ( P < 0.05 ). Moreover, the activities of key glycolytic enzymes, lactate, ATP, HK2, PKM2, LDHA, MCT4, GLUT1, GPR81 protein or mRNA expression, and morphological abnormalities of trophoblast cells improved significantly in the RSA mice after dydrogesterone or different doses of STW treatment ( P < 0.05 ). Conclusion. STW can promote aerobic glycolysis in trophoblast cells of RSA mouse embryos, thereby improving the microenvironment of the maternal-fetal interface and enhancing embryo implantation.

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Changes and clinical significance of peripheral blood helper T lymphocyte and natural killer (NK) cells in unexplained recurrent spontaneous abortion (URSA) patients after abortion and successful pregnancy

Cited by 16 publications

References 20 publications

Association of B Cells with Idiopathic Recurrent Pregnancy Loss: A Systematic Review and Meta-Analysis

Association of B Cells with Idiopathic Recurrent Pregnancy Loss: A Systematic Review and Meta-Analysis

Intralipid therapy for unexplained recurrent miscarriage and implantation failure: Case-series and literature review

Shoutai Wan Improves Embryo Survival by Regulating Aerobic Glycolysis of Trophoblast Cells in a Mouse Model of Recurrent Spontaneous Abortion

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