Changes in anatomy and root cell ultrastructure of soybean genotypes under manganese stress

Lavres, José; Malavolta, E.; Nogueira, Neusa de Lima; Moraes, Milton Ferreira de; Reis, André Rodrigues dos; Rossi, Mônica Lanzoni; Cabral, Cristiane da Silva

doi:10.1590/s0100-06832009000200017

Cited by 19 publications

(11 citation statements)

References 31 publications

(27 reference statements)

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“…Antioxidant enzymes and proteins were also extracted from roots. At the end of the experiment, when the symptoms of Ni toxicity had worsened (7 days of exposure to Ni), the first fully developed trifoliate was collected for leaf diagnosis (Lavres Junior et al, 2009 and to characterize the symptoms of Ni toxicity (leaf morphology and scanning electron microscopy).…”

Section: Growth Conditions and Experimental Designmentioning

confidence: 99%

A glimpse into the physiological, biochemical and nutritional status of soybean plants under Ni-stress conditions

Reis

Barcelos

Osório

et al. 2017

Environmental and Experimental Botany

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Nickel (Ni) toxicity has been reported to decrease productivity in soybean (Glycine max L.). However, soybean responses to Ni toxicity are not well understood. The aim of the present study was to describe Ni toxicity in soybean plants through physiological, nutritional, and ultrastructural analyses. Plants were grown in nutrient solution containing increasing Ni concentrations (0, 0.05, 0.1, 0.5, 10, and 20 μmol L −1), and nutritional, anatomical, physiological and biochemical features were determined. The results revealed previously unreported detrimental effects of Ni toxicity on soybean plants. CO 2 assimilation rates, stomatal conductance and transpiration decreased, resulting in lower biomass in soybean plants exposed to the highest Ni levels. Nitrate reductase activity increased with up to 0.05 μmol L −1 Ni and then decreased, indicating halted N-metabolism. Urease activity increased with increasing Ni availability in the nutrient solution, and peroxidase and superoxide dismutase activities were higher in plants grown at higher Ni levels. Leaf epidermal thickness (abaxial and adaxial), as well as root xylem and phloem diameter, decreased starting at 0.1 μmol L −1 Ni. Mean Ni concentrations varied from 77.5 to 17,797.4 mg kg −1 in roots and 2.3 to 16,774.5 mg kg −1 in shoots. Soybean plants exhibited symptoms of Ni toxicity starting at 0.1 μmol L −1 Ni, presenting mean shoot Ni concentration of 28.9 mg kg −1 , along with leaf water loss until complete drying. The results contribute to our understanding of several physiological, biochemical and histological mechanisms of Ni toxicity in soybean, which is still poorly understood.

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Section: Growth Conditions and Experimental Designmentioning

confidence: 99%

A glimpse into the physiological, biochemical and nutritional status of soybean plants under Ni-stress conditions

Reis

Barcelos

Osório

et al. 2017

Environmental and Experimental Botany

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“…Barley showed leaf necrosis and reduced growth at foliar Mn concentrations between 170 and 280 μg g -1 [ 49 ]. Similarly, soybean [ 50 ], cowpea and beans [ 20 ] are very sensitive to Mn toxicity. That some of the rice genotypes in this screening study showed very little damage despite excessively high shoot Mn concentration confirms previous studies [ 9 , 11 – 13 ] stating that rice can be considered as a rather Mn tolerant species.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide association study to identify candidate loci and genes for Mn toxicity tolerance in rice

et al. 2018

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Manganese (Mn) is an essential micro-nutrient for plants, but flooded rice fields can accumulate high levels of Mn2+ leading to Mn toxicity. Here, we present a genome-wide association study (GWAS) to identify candidate loci conferring Mn toxicity tolerance in rice (Oryza sativa L.). A diversity panel of 288 genotypes was grown in hydroponic solutions in a greenhouse under optimal and toxic Mn concentrations. We applied a Mn toxicity treatment (5 ppm Mn2+, 3 weeks) at twelve days after transplanting. Mn toxicity caused moderate damage in rice in terms of biomass loss and symptom formation despite extremely high shoot Mn concentrations ranging from 2.4 to 17.4 mg g-1. The tropical japonica subpopulation was more sensitive to Mn toxicity than other subpopulations. Leaf damage symptoms were significantly correlated with Mn uptake into shoots. Association mapping was conducted for seven traits using 416741 single nucleotide polymorphism (SNP) markers using a mixed linear model, and detected six significant associations for the traits shoot manganese concentration and relative shoot length. Candidate regions contained genes coding for a heavy metal transporter, peroxidase precursor and Mn2+ ion binding proteins. The significant marker SNP-2.22465867 caused an amino acid change in a gene (LOC_Os02g37170) with unknown function. This study demonstrated significant natural variation in rice for Mn toxicity tolerance and the possibility of using GWAS to unravel genetic factors responsible for such complex traits.

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“…1. The soil in the area is classified as having a clayey textural class, which is kaolinitic and thermic, and is a Typic Haplorthox soil (Soil Survey Staff 2014), which corresponds to Oxisols (Jahn et al 2006). Prior to the field experiment, the soil was sampled and chemical and texture analyses were undertaken according to van Raij et al (2001) and Donagema et al (2017), respectively (Table S1).…”

Section: Site Descriptionmentioning

confidence: 99%

“…Different lower-case letters indicate significant differences between lime doses and different capital letters indicate significant differences between crop systems according to the LSD test at p ≤ 0.05. Error bars represent the standard error of the mean (n = 4) compromise the nutritional plant status (Lavres Junior et al 2009), although deficiency symptoms were not observed during the experiment conduction.…”

Section: −mentioning

confidence: 99%

Soybean in Crop Rotation with Maize and Palisade Grass Intercropping Enhances the Long-term Effects of Surface Liming in No-till System

Bossolani

Santos

Meneghette

et al. 2020

J Soil Sci Plant Nutr

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Tropical and subtropical soils are characterized by low natural fertility. Therefore, further research needs to be undertaken to increase surface liming efficiency, improve soil quality, and promote crop development. The low solubility of lime has hampered acidity correction in deeper layers, particularly if a no-tillage system is used. However, surface liming efficiency can be improved if efficient crop rotation programs are implemented. Therefore, the main aim of this study was to investigate the long-term effects of lime doses on soil fertility and the cascading effects of these changes in soybean nutrition and productivity after maize monocrop or intercropped with palisade grass during three growing seasons. In a long-term experiment (started in 2019), soybean was cultivated under four lime doses (0, 2000, 4000, and 6000 kg ha −1) applied to soil surface in two crop systems (after maize monocrop or intercropped maize with palisade grass) during three consecutive agricultural years (2016-2019). Liming reduced soil acidic parameters and increased P, Ca 2+ , Mg 2+ , and S-SO 4 2− availability down the soil profile, which improved soybean nutrition and grain yield, particularly when it followed intercropped maize. Intercropping exploits species complementarities, which results in increasing the effectiveness of surface liming under no-tillage systems, contributing to higher soybean yields in crop rotation.

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Changes in anatomy and root cell ultrastructure of soybean genotypes under manganese stress

Cited by 19 publications

References 31 publications

A glimpse into the physiological, biochemical and nutritional status of soybean plants under Ni-stress conditions

A glimpse into the physiological, biochemical and nutritional status of soybean plants under Ni-stress conditions

Genome-wide association study to identify candidate loci and genes for Mn toxicity tolerance in rice

Soybean in Crop Rotation with Maize and Palisade Grass Intercropping Enhances the Long-term Effects of Surface Liming in No-till System

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