Changes in antibiotic resistance genes and mobile genetic elements during cattle manure composting after inoculation with Bacillus subtilis

Duan, Manli; Zhang, Yuhua; Zhou, Beibei; Wang, Quanjiu; Gu, Jie; Liu, Guohuan; Qin, Zhenlun; Z, Li

doi:10.1016/j.biortech.2019.122011

Cited by 101 publications

(38 citation statements)

References 42 publications

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“…In this study, the composting process removes most of ARGs and MGEs by 22.8-99.7%, but compound microbial inoculants only increased the removal rate of part of ARGs by 3.7-23.8% in compost. This is consistent with the research results of Duan et al (2019). And composting could not remove the ARGs completely.…”

Section: Discussionsupporting

confidence: 91%

“…At the same time, our research also indicated that the relative abundance of Firmicutes decreased, but Bacteroidetes, Proteobacteria and Actinobacteria were increased in the maturation phase of compost. Duan et al (2019) pointed out that Firmicutes are the main cause of ARGs changes during cattle manure composting. It has been reported that a decrease in the relative abundance of Firmicutes might explain the removal of most ARGs in compost (Huerta et al, 2013;Stegmann et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…And it was perforated with one ventilation hole (2 × 2 cm) on the side of the insulation wall. The composting device is very similar to the research from Duan et al (2019). Two composting experiments were labeled as CK (swine manure + sawdust) and AB (swine manure + sawdust + 1% compound microbial agents − Bacillus subtilis: Bacillus licheniformis: Bacillus megaterium: Yeasts = 1:1:1:2).…”

Section: Composting Processmentioning

confidence: 99%

“…And the impact of inoculation on ARGs is even less studied. Only Duan et al (2019) studied the effects of Bacillus subtilis on ARGs and mobile genetic elements (MGEs) during cattle manure composting. But in practical application, the addition of compound microbial agents is often used to accelerate the composting process.…”

Section: Introductionmentioning

confidence: 99%

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Swine Manure Composting With Compound Microbial Inoculants: Removal of Antibiotic Resistance Genes and Their Associations With Microbial Community

Cao

et al. 2020

Front. Microbiol.

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In this study, compound microbial inoculants, including three Bacillus strains and one Yeast strain, were inoculated into swine manure composting to explore the effects on antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs), microbial community structure, and pathogenic bacteria. The results indicated that the abundances of the detected ARGs ranged from 3.6 × 10 3 to 1.13 × 10 8 copies/g. The ARGs with the highest abundance was sul2, and the lowest was blaCTX. Composting removes most of the ARGs and MGEs by 22.8-99.7%. These ARGs were significantly reduced during the thermophilic phase of compost. The removal rate of ARGs at the different layers of compost pile was different as follows: middle layer > upper layer > lower layer. But some ARGs proliferated significantly in the maturation phase of compost, especially the sulfonamide resistance genes. Compound microbial inoculants increased the temperature of compost, accelerated water loss, nitrogen fixation, and increased the removal rate of β-lactamase resistance genes, the transposon gene tn916 and part of tetracycline resistance genes by 3.7-23.8% in compost. Compound microbial inoculants changed the community structure and increased the Bacillus abundance in the thermophilic phase of compost. And it was helpful for removing pathogens during composting. The addition of compound microbial inoculants causes the decrease of Firmicutes and the increase of Bacteroidetes, which may be related to the removal and proliferation of ARGs.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Composting Processmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Swine Manure Composting With Compound Microbial Inoculants: Removal of Antibiotic Resistance Genes and Their Associations With Microbial Community

Cao

et al. 2020

Front. Microbiol.

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“…It was found that 95.0% of tylosin antibiotic fermentation residues, 75.8% of ARGs, and 98.5% of mobile genetic elements can be removed by composting after exposure above 60°C for at least 6 days. Duan et al (2019) studied the changes of ARGs and flow genes in the composting process of cow manure inoculated with Bacillus subtilis at four levels (0%, 0.5%, 1%, and 2% w/w compost). The result showed that the composting had a great impact on ARGs than bacillus subtilis.…”

Section: Compostingmentioning

confidence: 99%

Emerging pollutants—Part II: Treatment

Liu

Zhang

Chang

2020

Water Environment Research

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Emerging pollutants (EPs) refer to a class of pollutants, which are emerging in the environment or recently attracted attention. EPs mainly include pharmaceutical and personal care products (PPCPs), endocrine-disrupting chemicals (EDCs), and antibiotic resistance genes (ARGs). EPs have potential threats to human health and ecological environment. In recent years, the continuous detections of EPs in surface and ground water have brought huge challenges to water treatment and also made the treatment of EPs become an international research hotspot. This paper summarizes some research results on EPs treatment published in 2019. This paper may be helpful to understand the current situations and development trends of EP treatment technologies.

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Antimicrobial resistance in integrated agroecosystems: State of the science and future opportunities

Howe

Soupir

2021

J of Env Quality

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As the Journal of Environmental Quality (JEQ) celebrates 50 years of publication, the division of environmental microbiology is one of the newest additions to the journal. During this time, significant advances in understanding of the interconnected microbial community and impact of the microbiome on natural and designed environmental systems have occurred. In this review, we highlight the intractable challenge of antimicrobial resistance (AMR) on humans, animals, and the environment, with particular emphasis on the role of integrated agroecosystems and by highlighting contributions published in JEQ. From early studies of phenotypic resistance of indicator organisms in waters systems to current calls for integrating AMR assessment across “One Health,” publications in JEQ have advanced our understanding of AMR. As we reflect on the state of the science, we emphasize future opportunities. First, integration of phenotypic and molecular tools for assessing environmental spread of AMR and human health risk continues to be an urgent research need for a one health approach to AMR. Second, monitoring AMR levels in manure is recommended to understand inputs and potential spread through agroecosystems. Third, baseline knowledge of AMR levels is important to realize the impact of manure inputs on water quality and public health risk; this can be achieved through background monitoring or identifying the source‐related genes or organisms. And finally, conservation practices designed to meet nutrient reduction goals should be explored for AMR reduction potential.

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Changes in antibiotic resistance genes and mobile genetic elements during cattle manure composting after inoculation with Bacillus subtilis

Cited by 101 publications

References 42 publications

Swine Manure Composting With Compound Microbial Inoculants: Removal of Antibiotic Resistance Genes and Their Associations With Microbial Community

Swine Manure Composting With Compound Microbial Inoculants: Removal of Antibiotic Resistance Genes and Their Associations With Microbial Community

Emerging pollutants—Part II: Treatment

Antimicrobial resistance in integrated agroecosystems: State of the science and future opportunities

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