Changes in apolipoprotein E-containing high-density lipoprotein (HDL) have little impact on HDL-cholesterol measurements using homogeneous assays in normolipidemic and dyslipidemic subjects

Sasamoto, Kenta; Hirayama, Satoshi; Kon, Mika; Seino, Utako; Ueno, Toshihide; Nagao, Yuki; Hirayama, Akiko; Isshiki, Miwa; Idei, Mayumi; Yano, Kouji; Miida, Takashi

doi:10.1016/j.cca.2017.04.018

Cited by 4 publications

(2 citation statements)

References 31 publications

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“…These findings are somewhat paradoxical as both LPS and LTA are known to incorporate into HDL 21 , 22 , and studies have shown that HepG2 cells can internalize HDL particles 35 . Furthermore, since LDLR can bind to apoE 36 , which is found on a subset of HDL particles 37 , it was expected that some uptake of HDL and incorporated bacterial lipids might occur through LDLR. Our observation that blocking LDLR has no effect on LPS or LTA uptake in the presence of HDL demonstrates that HDL does not directly contribute to uptake of these bacterial lipids through LDLR, but does not exclude an indirect contribution to the sequestration and/or transfer of bacterial lipids prior to such uptake.…”

Section: Discussionmentioning

confidence: 99%

Low-density lipoprotein (LDL)-dependent uptake of Gram-positive lipoteichoic acid and Gram-negative lipopolysaccharide occurs through LDL receptor

Grin

Dwivedi

Chathely

et al. 2018

Sci Rep

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Lipoteichoic acid (LTA) and lipopolysaccharide (LPS) are bacterial lipids that stimulate pro-inflammatory cytokine production, thereby exacerbating sepsis pathophysiology. Proprotein convertase subtilisin/kexin type 9 (PCSK9) negatively regulates uptake of cholesterol by downregulating hepatic lipoprotein receptors, including low-density lipoprotein (LDL) receptor (LDLR) and possibly LDLR-related protein-1 (LRP1). PCSK9 also negatively regulates Gram-negative LPS uptake by hepatocytes, however this mechanism is not completely characterized and mechanisms of Gram-positive LTA uptake are unknown. Therefore, our objective was to elucidate the mechanisms through which PCSK9 regulates uptake of LTA and LPS by investigating the roles of lipoproteins and lipoprotein receptors. Here we show that plasma PCSK9 concentrations increase transiently over time in septic and non-septic critically ill patients, with highly similar profiles over 14 days. Using flow cytometry, we demonstrate that PCSK9 negatively regulates LDLR-mediated uptake of LTA and LPS by HepG2 hepatocytes through an LDL-dependent mechanism, whereas LRP1 and high-density lipoprotein do not contribute to this uptake pathway. Bacterial lipid uptake by hepatocytes was not associated with cytokine production or hepatocellular injury. In conclusion, our study characterizes an LDL-dependent and LDLR-mediated bacterial lipid uptake pathway regulated by PCSK9, and provides evidence in support of PCSK9 inhibition as a potential therapeutic strategy for sepsis.

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Section: Discussionmentioning

confidence: 99%

Low-density lipoprotein (LDL)-dependent uptake of Gram-positive lipoteichoic acid and Gram-negative lipopolysaccharide occurs through LDL receptor

Grin

Dwivedi

Chathely

et al. 2018

Sci Rep

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“…At the end of the experiment, blood plasma levels of total cholesterol (TC), HDL-cholesterol (HDL-C), triglyceride (TG), and glucose were measured under the condition of free feeding, by using enzymatic assay kits for lipid parameters provided by Sekisui Medical Co., Ltd., Tokyo and by Glucose CII Test Wako (GOD method, Wako Pure Chemical Industries, Ltd., Tokyo) for blood glucose level. The presence of apolipoprotein E-containing HDL in rat plasma may not likely influence the HDL assay significantly ( 21 ) but the assay system was validated for linearity and selectivity for rat plasma. Non-HDL-cholesterol (non-HDL-C) was calculated as [TC]–[HDL-C].…”

Section: Methodsmentioning

confidence: 99%

Sichuan dark tea improves lipid metabolism and prevents aortic lipid deposition in diet-induced atherosclerosis model rats

Sugimoto²,

Tsuboi³

et al. 2022

Front. Nutr.

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Background and aimsSichuan dark tea (ST), Zangcha, is a traditional fermented Chinese tea found in Sichuan and Tibet and claimed for beneficial effects against lifestyle-related metabolic disorders. We examined the effects of ST on lipid metabolism and atherosclerosis.Methods and resultsSichuan dark tea was given to fat-rich diet-induced atherosclerosis model rats in comparison with dark-fermented Chinese Pu-erh tea (PT) and Japanese green tea (GT). After 8 weeks of feeding, ST and PT induced an increase in high-density lipoprotein (HDL)-cholesterol and a decrease in glucose, and ST decreased triglyceride in plasma. ST also induced low pH in the cecum. There was no significant change in their body weight among the fat-feeding groups but a decrease was found in the visceral fat and liver weight in the ST group. Accordingly, ST reduced lipid deposition in the aorta in comparison with PT and GT. ST increased mRNA of LXRα, PPARα, PPARγ, and ABCA1 in the rat liver. The extract of ST stimulated the AMPK pathway to increase the expression of ABCA1 in J774 cells and increased expression of lipoprotein lipase and hormone-sensitive lipase in 3T3L1 cells, consistent with its anti-atherogenic effects in rats. High-performance liquid chromatography analysis showed unique spectra of original specific compounds of caffeine and catechins in each tea extract, but none of them was likely responsible for these effects.ConclusionSichuan dark tea increases plasma HDL and reduces plasma triglyceride to decrease atherosclerosis through AMPK activation. Further study is required to identify specific components for the effects of this tea preparation.

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Soluble LR11 competes with amyloid β in binding to cerebrospinal fluid–high-density lipoprotein

Yano

Hirayama

Misawa

et al. 2019

Clinica Chimica Acta

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Changes in apolipoprotein E-containing high-density lipoprotein (HDL) have little impact on HDL-cholesterol measurements using homogeneous assays in normolipidemic and dyslipidemic subjects

Cited by 4 publications

References 31 publications

Low-density lipoprotein (LDL)-dependent uptake of Gram-positive lipoteichoic acid and Gram-negative lipopolysaccharide occurs through LDL receptor

Low-density lipoprotein (LDL)-dependent uptake of Gram-positive lipoteichoic acid and Gram-negative lipopolysaccharide occurs through LDL receptor

Sichuan dark tea improves lipid metabolism and prevents aortic lipid deposition in diet-induced atherosclerosis model rats

Soluble LR11 competes with amyloid β in binding to cerebrospinal fluid–high-density lipoprotein

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