Changes in Bacterial Communities of Kumamoto Oyster Larvae During Their Early Development and Following Vibrio Infection Resulting in a Mass Mortality Event

Dai, Wenfang; Ye, Jing; Xue, Qinggang; Liu, Sheng; Xu, Hongqiang; Liu, Minhai; Zhang, Lin

doi:10.1007/s10126-022-10178-0

Cited by 5 publications

(4 citation statements)

References 77 publications

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“…At these times, larvae would be at the highest stocking densities and physiologically at their most vulnerable [29,30]. Alterations in oyster-associated bacterial communities are commonly reported during disease and mortality events [27,[31][32][33]. Interestingly, the larvae in small-scale airlift systems had similar mortality rates to controls.…”

Section: Discussionmentioning

confidence: 96%

“…Control tanks maintained higher overnight water temperatures than small-scale systems. These warmer water temperatures may alter microbial community dynamics within the system [27,28]. These community shifts would likely be most pronounced in the early days of the trial where water was only changed every other day, giving the bacteria a relatively long period for replication.…”

Section: Discussionmentioning

confidence: 99%

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Growth of Oyster (Crassostrea virginica) Larvae in Small-Scale Systems Using an Algae Concentrate Food Source

Tarnecki,

Cleveland,

Capps

et al. 2024

Aquaculture Research

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Replicated studies are advantageous for optimizing larval rearing of the Eastern oyster (Crassostrea virginica) and increasing the availability of high-quality seed for the continued expansion of the U.S. oyster aquaculture industry. Although small-scale systems using live algal feeds have been used successfully, rearing larvae on algae concentrate presents additional challenges. To determine the feasibility of rearing oyster larvae in small-scale systems using algae concentrate, oyster larvae were raised for 2 weeks in replicate control (1,000 L) and microcosm (17 L) tanks. Five aeration strategies were tested in the microcosms in two separate trials. Results of this study indicate similar survival in small systems compared to controls through the appearance of eyed larvae. Accumulated algae and pink biofilm formation in microcosms using polyvinyl chloride (PVC) airlifts suggest that this aeration strategy is undesirable. One- and 5-mL air injectors maintained higher overnight oxygen levels than controls. The recovery of more eyed larvae after 14 dpf in control systems may be the result of significant temperature fluctuations in microcosms. Overall, this study demonstrates that algae concentrate can be used to rear oyster larvae in small-scale systems, providing a live feed alternative that saves space and labor in replicated studies.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Growth of Oyster (Crassostrea virginica) Larvae in Small-Scale Systems Using an Algae Concentrate Food Source

Tarnecki,

Cleveland,

Capps

et al. 2024

Aquaculture Research

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show abstract

“…Vibrios including the potential pathogenic V. alginolyticus and V. mediterranei, are ubiquitous in marine and estuarine aquatic systems [6,9,47]. It is impossible to eliminate the potential pathogenic microorganisms from the bivalve hatcheries [7,9,47]. Moreover, there are always no biosecurity protocols, hygiene plans, or risk-based surveillance schemes for these family-style bivalve hatcheries in China [46].…”

Section: Discussionmentioning

confidence: 99%

“…Epidemiological investigation of hatchery epidemics indicated that Ostreid herpesvirus 1 (OsHV-1), Vibrio algniolyticus, Vibrio mediterranei, and Pseudoalteromonas spp. were the potential pathogenic microorganisms [6][7][8][9]. The poor facilities to eliminate pathogens from seawater, poor biosecurity concepts during the operation, and management were assumed to be responsible for the insufficiency of disease prevention [6,10].…”

Section: Introductionmentioning

confidence: 99%

The Probiotic Bacillus hwajinpoensis Colonizes the Digestive System of Crassostrea gigas Larvae and Protects Them from Vibrio alginolyticus Infection

Zheng,

Huang,

Zhang

et al. 2023

Microorganisms

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The Pacific oyster Crassostrea gigas is one of the most important cultured marine species around the world. Production of Pacific oysters in China has depended primarily on hatchery produced seeds since 2016, with the successful introduction and development of triploid oysters. However, the seed supply of Pacific oysters is threatened by recurring mass mortality events in recent years. Vibriosis is the most commonly encountered disease associated with intensive oyster culture in hatcheries and nurseries. Vibrio alginolyticus and Bacillus hwajinpoensis were the two strains with pathogenic and probiotic effects, respectively, identified during the Pacific oyster larvae production. To monitor their colonization process in Pacific oyster larvae, green fluorescent protein (GFP) and red fluorescent protein (RFP) were labeled to the pathogenic V. alginolyticus and the probiotic B. hwajinpoensis stain, respectively. The pathogenic and probiotic effects of the two strains during the colonization process were then assessed. Stabile expression of GFP and RFP were observed in corresponding stains, and the capabilities of growth, biofilm formation and in vitro adhesion of GFP- and RFP- tagged stains were not significantly different from those of the wild-type strains. Usage of probiotics of 105 CFU/mL significantly inhibited the growth of pathogenic V. alginolyticus and reduced the mortality of D-sharped larvae. Both the pathogenic and probiotic strains employed a similar route to enter and colonize the oyster larvae, which indicates that competing with pathogens for binding and spreading sites were one of the mechanisms of B. hwajinpoensis to provide the probiotic effects to oyster larvae. In summary, employment of fluorescence-tagged pathogenic and probiotic strains simultaneously provides us with an excellent bioassay model to investigate the potential mechanisms of probiotics.

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Acute Salinity Stress Disrupts Gut Microbiota Homeostasis and Reduces Network Connectivity and Cooperation in Razor Clam Sinonovacula constricta

Dai,

Zhang,

Dong

et al. 2023

Mar Biotechnol

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Changes in Bacterial Communities of Kumamoto Oyster Larvae During Their Early Development and Following Vibrio Infection Resulting in a Mass Mortality Event

Cited by 5 publications

References 77 publications

Growth of Oyster (Crassostrea virginica) Larvae in Small-Scale Systems Using an Algae Concentrate Food Source

Growth of Oyster (Crassostrea virginica) Larvae in Small-Scale Systems Using an Algae Concentrate Food Source

The Probiotic Bacillus hwajinpoensis Colonizes the Digestive System of Crassostrea gigas Larvae and Protects Them from Vibrio alginolyticus Infection

Acute Salinity Stress Disrupts Gut Microbiota Homeostasis and Reduces Network Connectivity and Cooperation in Razor Clam Sinonovacula constricta

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