Changes in biochemical analytes in female dogs with subclinical Ancylostoma spp. infection

Schmidt, Elizabeth Moreira dos Santos; Tvarijonaviciute, Asta; Martı́nez-Subiela, Silvia; Cerón, José J.; Eckersall, P.D.

doi:10.1186/s12917-016-0833-2

Cited by 11 publications

(11 citation statements)

References 38 publications

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“…The increased incidence in male as compared to female dogs could be due to the different pathophysiology of worms in the two genders (Schmidt et al 2016). The increased incidence in young dogs as compared to adults, meanwhile, could be due to lower immunity in young pups and trans-placental infection as well as trans-mammary transmission of Ancylostoma eggs (Lefkaditis 2001;RamirezBarrios et al 2004).…”

Section: Discussionmentioning

confidence: 99%

First report of the molecular detection of Ancylostoma caninum in Lahore, Pakistan: the threat from pets

Rehman¹,

Akhtar²,

Akbar³

et al. 2017

Vet. Med. - Czech

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ABSTRACT:The molecular prevalence of Ancylostoma caninum was determined in stray and pet dogs in Lahore, Pakistan from July 2014 to August 2015. A total of 500 dog faecal samples were first evaluated using a sedimentation technique and further through a PCR assay targeting the ITS-2 region of the A. caninum genome. Overall, 130 (26%) samples were positive for hookworm infestation by microscopic examination. Only microscopically positive samples were processed for PCR and 89 (17.6%) were positive for A. caninum. Sequence analysis of amplicons showed 100% homology with A. caninum and the genotypes clustered in one clade with Brazilian A. caninum hookworms. There was a significantly (P < 0.05) increased prevalence in male dogs younger than six months old. Labrador retrievers had higher A. caninum incidence compared to German shepherds and other breeds. To the best of our knowledge, this is the first report of the molecular prevalence of A. caninum in dogs in Pakistan. The novel results of the present study allow us to conclude that A. caninum is more prevalent in pet dogs, especially puppies, and this can be a potential threat for humans that come into contact with such animals. Therefore, the routine monitoring of pets, especially the more susceptible breeds, is essential for disease control.

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Section: Discussionmentioning

confidence: 99%

First report of the molecular detection of Ancylostoma caninum in Lahore, Pakistan: the threat from pets

Rehman¹,

Akhtar²,

Akbar³

et al. 2017

Vet. Med. - Czech

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“…Thus, a study of the blood of dogs subclinically affected by Ancylostoma spp. revealed a significant increase in the concentration of C-reactive protein and haptoglobin, a significant decrease in iron and albumin (Schmidt et al, 2016).…”

Section: Discussionmentioning

confidence: 97%

“…Scientific publications demonstrate results of studies of haematological parameters of dogs infected with various co-infections of parasites, including the gastrointestinal (Schmidt et al, 2016;Rouf et al, 2017;Kumar, 2021). However, data and analysis of changes in haematological parameters in co-infection with T. vulpis + T. canis, as an important aspect of studying the pathogenesis of infection, have not been described.…”

Section: Introductionmentioning

confidence: 99%

Co-infection of Trichuris vulpis and Toxocara canis in different aged dogs: Influence on the haematological indices

Saichenko

Antipov

Bakhur³

et al. 2021

Biosys. divers.

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Trichuris vulpis and Toxocara canis are worldwide parasitic nematodes affecting dogs and mammals of the Canine family. Due to the special structure of the shells, the eggs of these geohelminths can maintain their viability in soil, sand, water and the environment for a long time. The study involved young (6–12 months old) and adult (1.5–8.0 years old) dogs affected by co-infection of T. vulpis + T. canis; the control group consisted of dewormed healthy animals of the same age. Parasitological examination of dogs' faeces was performed using a "Counting Chamber for Ovoscopic Researches"; morphological parameters, indicators of nutrient metabolism, mineral metabolism and activity of enzymatic systems were determined in blood and serum. According to the results of parasitological research on the dogs, it was found that young animals are more prone to toxocarosis, and adults – trichurosis. The co-infection of nematodes T. vulpis + T. canis in dogs develops several changes in haematological parameters: a significant decrease in erythrocytes, haemoglobin, MCV, MCH, and haematocrit, leukocytosis with basophils and eosinophilia in young infected animals; and eosinophilia and basophilia (15.9 times) in adults, compared with healthy dogs of the same age. Among the changes in serum biochemical parameters, young infected dogs showed a decrease in the concentration of total protein and albumin content, an increase in the content of "acute phase" proteins (α-1, α-2 and β-globulins), an increase in cholesterol and total bilirubin; in adult infected dogs, a decrease in albumin content, an increase in the content of α-1, α-2, and β-globulins, an increase in the concentration of cholesterol and total bilirubin, a decrease in the concentration of urea in comparison with healthy animals were determined. Also, among the indicators of mineral metabolism, a decrease in the concentration of calcium and magnesium in the serum was found in young infected dogs. Among the changes in the enzyme metabolism in the serum of infected dogs, there was an increase in the activity of all studied enzymes in animals of both research groups: ALT, AST, α-amylase, GGT and ALP. Thus, co-infection with nematodes T. vulpis + T. canis has a complex pathogenic effect on the body of dogs of all ages, which manifested itself in multiple changes in haematological parameters. In the future, the authors’ team plan to develop comprehensive measures to combat nematode infections in different living conditions of dogs, taking into account the results of the current research.

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“…However, when investigating a low‐grade inflammatory condition, using a hs‐CRP assay that accurately measures low concentrations may be necessary. Also, while factors such as subclinical disease can largely be ignored when interpreting CRP concentrations from dogs with severe systemic inflammation, they might need to be considered when evaluating low‐grade inflammatory conditions 48 …”

Section: Measurement Of Canine Crpmentioning

confidence: 99%

Measurement and clinical applications of C‐reactive protein in gastrointestinal diseases of dogs

Covin

Steiner

2022

Veterinary Clinical Pathol

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C‐reactive protein (CRP) is a positive acute‐phase protein, serum concentrations of which increase nonspecifically in response to inflammatory processes of the dog. As such, it can aid in the identification of inflammatory disease and, maybe more importantly, the objective monitoring of disease progression. In dogs, CRP is frequently used to evaluate dogs with gastrointestinal diseases, such as chronic inflammatory enteropathies (also termed idiopathic inflammatory bowel disease), acute pancreatitis, canine parvovirus infection, hepatic disease, acute abdomen, and protein‐losing enteropathy. The diversity of the assays available to measure CRP in dogs is nearly as numerous as the diseases in which serum concentrations of this protein are increased. Assay methodologies include laser nephelometric immunoassays, enzyme‐linked immunosorbent assays, immunoturbidimetric assays, and time‐resolved immunofluorometric assays. While many of these assays are acceptable for clinical use in the dog, the same assay and analyzer should be used to measure a patient's CRP concentration longitudinally. By looking at the uses of CRP in human gastroenterology, including reducing the duration of antibiotic therapy, the veterinary profession can gain insight into novel ways in which serum CRP concentration measurements might be applied in veterinary medicine in the future.

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Changes in biochemical analytes in female dogs with subclinical Ancylostoma spp. infection

Cited by 11 publications

References 38 publications

First report of the molecular detection of Ancylostoma caninum in Lahore, Pakistan: the threat from pets

First report of the molecular detection of Ancylostoma caninum in Lahore, Pakistan: the threat from pets

Co-infection of Trichuris vulpis and Toxocara canis in different aged dogs: Influence on the haematological indices

Measurement and clinical applications of C‐reactive protein in gastrointestinal diseases of dogs

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