Changes in cell gene expression in human leukemic cells persistently infected with vaccinia virus

Two-colour immunocytochemical staining of gamma (γ) and epsilon (ε) type haemoglobin in fetal red cells

“…The latter mechanism has been proven for the switch from fetal Hb to adult Hb (Dzierzak and Medvinsky, 1995;Raich et al, 1990). Also, in vitro cultured K562 cells can be induced to produce both and chains which indicate the genetic possibility of switching at the single-cell level (Pogo et al, 1991).…”

“…Such questions can be answered by immunocytochemical methods. So far, these methods focus on the demonstration of fetal Hb, as applied in prenatal diagnosis (Bohmer et al, 1997;Pogo et al, 1991;Zheng et al, 1996).…”

Effects of Astragalus polysaccharide on the erythroid lineage and microarray analysis in K562 cells

“…It is localized to chromosome 7q11.23. It has been implicated in the recruitment of transcription complexes to promoters, containing an initiator sequence overlapping the transcription start site (Pogo et al, 1991). Diminishing TFII-I activity increased ␤-globin gene expression in erythroid cell lines.…”

Molecular Characterization of Jurkat Cells Persistently Infected with Vaccinia Virus Mutant Vp811

“…In addition to cellular changes, viral mutants can be isolated from these populations [8,9]. Furthermore, persistently infected K562 cells show increased expression of fetal hemoglobin (mainly G-Á type) [6] and persistently infected Jurkat cells upregulate the expression of IL-2, IL-2R· and IL-6 but not of interferon-Á (IFNÁ) nor of IL-1ß [7]. These cells are also capable to transactivate the LTR gene of HIV-1 virus (HIV-1LTR) [10] due to the activation of NFÎß and NFAT elements present in HIV-1LTR [11].…”

“…VV is cytocidal to most cells; however, persistent infections can be established in murine Friend erythroleukemia (FEL) cells [4], human erythroleukemia (K562) cells [5,6] and in a human T cell leukemia cell line (Jurkat) with the IHD W strain of VV [7]. Studies of the persistently infected FEL cells demonstrated that these cells are less tumorigenic than the parental cells, resist superinfection, show chromosomal abnormalities and display a high level of differentiation after serial passage in vitro [4].…”