Changes in cell size during the cooling, warming and post-thawing periods of the freeze-thaw cycle

Griffiths, J. B.; Cox, CI; Beadle, David J.; Hunt, Charles J.; Reid, David

doi:10.1016/0011-2240(79)90024-5

Cited by 35 publications

(14 citation statements)

References 34 publications

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“…However, excessively higher cooling rates (e.g., 1000°C/min) in the presence of EG do result in potentially damaging cell volume excursions. Similar experimental findings of damaging cell volume excursions upon cooling and warming have been previously reported in other cell types such Chinese hamster ovary cells [29]. In addition, the theoretical simulations indicate that cell loss during cooling and warming is not primarily a result of intracellular ice formation.…”

Section: Simulation Of Intracellular Water Volume Flux During Coolingsupporting

confidence: 77%

Determination of Plasma Membrane Characteristics of Boar Spermatozoa and their Relevance to Cryopreservation1

Gilmore

Liu²,

Peter

et al. 1998

Biology of Reproduction

103

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The osmotic tolerance limits for boar spermatozoa were determined at 22 degrees C. These cells can swell to within 1.02 times and shrink to within 0.97 times their isosmotic volume and maintain > 70% motility. In the presence of an extender, cells can swell to within 1.1 times and shrink to within 0.97 times their isosmotic volume and maintain > 70% motility. Plasma membrane permeability coefficients were determined in the presence of 1 M dimethyl sulfoxide (DMSO), 1 M glycerol, and 2 M ethylene glycol (EG) at 22 degrees C. Hydraulic conductivity (Lp) was estimated to be 0.120+/-0.016 (mean+/-SEM), 0.138+/-0.006, and 0.204 +/-0.021 microm/min/atm in the presence of DMSO, glycerol, and EG, respectively, at 22 degrees C. Solute permeability (P[CPA]) was determined to be 0.930+/-0.118, 0.481+/-0.045, and 1.98+/-0.106 x 10(-3) cm/min, for DMSO, glycerol, and EG, respectively. Subsequent experiments were performed at 8 degrees C and 0 degrees C. Activation energies were calculated for Lp in the presence of glycerol and EG to be 7.20 and 11.51 Kcal/mol, respectively. The activation energies for P(CPA) were 4.06 and 7.48 Kcal/mol for glycerol and EG permeability, respectively. These membrane characteristics were used to calculate volume flux during addition and removal of cryoprotectant agents as well as during cooling and warming. In addition, the potential for intracellular ice formation during cooling and warming was calculated.

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Section: Simulation Of Intracellular Water Volume Flux During Coolingsupporting

confidence: 77%

Determination of Plasma Membrane Characteristics of Boar Spermatozoa and their Relevance to Cryopreservation1

Gilmore

Liu²,

Peter

et al. 1998

Biology of Reproduction

103

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“…Because intracellular water does not freeze at the temperatures used, an osmotic gradient forms, causing the cell to become dehydrated. Damage to cell membrane structure is associated with the dehydration, and irreversible cell damage results from the severe swelling that occurs when the tissue is returned to room temperature 24,30–32 . Despite the loss of most tissue structure by these cooling parameters, the residual status of the lesion permits the establishment of a neuroglial network without the formation of a cavity.…”

Section: Discussionmentioning

confidence: 99%

Support of axonal regrowth by endogenous mechanisms following spinal cord injury in adult rats

2001

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Because of its non-invasive nature and ease of regulation, a closely monitored cryogenic method of tissue injury was used to create a degree of spinal cord injury within which there would be an extended regrowth of axons. The parameters of cooling used in the present study resulted in an injury length of 1 cm through which 3 mm of measured axonal regrowth and 8 mm of observed regrowth occurred over a 56-day period in the ascending fibers of the dorsal column of the mature rat. This was associated with the development of a cellular matrix consisting of macrophages, macroglia and Schwann cells which gradually expands within the injured area initially dominated by macrophages. It is the authors' impression that the presence of a substantial microglial component within the macrophage population may be a significant factor in the success of the axonal regrowth. Under this influence and that of the invading axons, the astrocyte, which provides the immediate cell support to the growing axon, can be maintained in a functional state that is supportive and not obstructive to the axon, presumably through the recruitment of astrocyte precursors from an indigenous stem cell population. These tissue changes indicate that adult mammalian spinal cord tissue does have the capacity to develop on its own a matrix capable of supporting the regrowth of axons.

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“…(1972), Troyer (1974), McGrath et al (1975), Qkamoto et al (1975, Valcic (1975), Hildebrandt & Cocks (1976, Reid (1978), Mersey et al (1978, Morris & McGrath (1980), Reid et al (1980 and Reisman & Friedman (1981). This type of instrumentation has been applied to the study of a large spectrum of biological tissues, including plant cells (Geneves, 1955), unfertilized eggs (Sherman & Lin, 1958), bone marrow cells (Richards & Persidsky, 1961), erythrocytes (Kozlov & Teodorovich, 1966;Kuivenhoven, 1966;Rinfret, 1968;Diller et al, 1972;Diller, 1975Diller, , 1979, kidney tissue (Sherman, 1969;Diller & Schmitt, 1981), protozoa (Bychenkova et al, 1969), nematodes (LozinaLozinskii & Namatov, 1971), HeLa cells (McGrath et al., 1975), mouse ova (Leibo et al 1978), Chinese hamster ovary cells (Griffiths et al, 1979) and liposomes (Morris & McGrath, 1981).…”

Section: Review O F Cryomicroscopymentioning

confidence: 97%

Quantitative low temperature optical microscopy of biological systems

Diller¹

1982

Journal of Microscopy

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A comprehensive review is presented of low temperature optical microscopy techniques as applied to the study of freezing processes in biological systems. Emphasis is placed on analysis of physical and physiological parameters which were measured and/or controlled and the procedures for effecting such operations. Quantitative analysis of photomicrographs by digital computer processing is also discussed.

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Changes in cell size during the cooling, warming and post-thawing periods of the freeze-thaw cycle

Cited by 35 publications

References 34 publications

Determination of Plasma Membrane Characteristics of Boar Spermatozoa and their Relevance to Cryopreservation1

Determination of Plasma Membrane Characteristics of Boar Spermatozoa and their Relevance to Cryopreservation1

Support of axonal regrowth by endogenous mechanisms following spinal cord injury in adult rats

Quantitative low temperature optical microscopy of biological systems

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