Changes in circulating microRNAs levels with exercise modality

Abstract

Here, we studied muscle-specific and muscle-related miRNAs in plasma of exercising humans. Our aim was to determine whether they are affected by eccentric and/or concentric exercise modes and could be biomarkers of muscle injuries or possible signaling molecules. On two separate days, nine healthy subjects randomly performed two 30-min walking exercises, one downhill (high eccentric component) and one uphill (high concentric component). Perceived exertion and heart rate were higher during the uphill exercise, while subjective pain and ankle plantar flexor strength losses within the first 48-h were higher following the downhill exercise. Both exercises increased serum creatine kinase and myoglobin with no significant differences between conditions. Plasma levels of circulating miRNAs assessed before, immediately after, and at 2-, 6-, 24-, 48-, and 72-h recovery showed that 1) hsa-mir-1, 133a, 133b, and 208b were not affected by concentric exercise but significantly increased during early recovery of eccentric exercise (2 to 6 h); 2) hsa-mir-181b and 214 significantly and transiently increased immediately after the uphill, but not downhill, exercise. The muscle-specific hsa-mir-206 was not reliably quantified and cardiac-specific hsa-mir-208a remained undetectable. In conclusion, changes in circulating miRNAs were dependent on the exercise mode. Circulating muscle-specific miRNAs primarily responded to a downhill exercise (high eccentric component) and could potentially be alternative biomarkers of muscle damage. Two muscle-related miRNAs primarily responded to an uphill exercise (high exercise intensity), suggesting they could be markers or mediators of physiological adaptations.

Preconception diet or exercise intervention in obese fathers normalizes sperm microRNA profile and metabolic syndrome in female offspring

“…The mechanism by which exercise restores these microRNAs remains to be investigated. However, it has been shown in humans that even short bouts of exercise can alter the microRNA profile of serum (8,65). It should be noted that exercise interventions in founders (HE and HCE) were previously shown to result in the greatest improvements to embryo development (38) and insulin sensitivity in female offspring at 9 wk of age in this study.…”

Intracellular and extracellular microRNA: An update on localization and biological role

“…It is possible that there are mechanisms of preliminary miRNA concentration/accumulation by recipient cells. After the damage of muscle fibers due to physical exercise the levels of muscle-specific miR-1 and miR-133 was significantly elevated in the plasma, but became already normal after several hours (Uhlemann et al, 2012;Banzet et al, 2013). This observation indicates the existence of mechanisms of rapid utilization of extracellular miRISC, so that they are present in the bloodstream much less time than might be expected based on their stability in vitro (Turchinovich et al, 2011).…”

“…The strongest evidence in favor of non-selective release of AGOassociated c-miRNA is the fact that miRNA content in cell culture media correlates with the level of cell death (Turchinovich et al, 2011). Likewise, many in vivo studies have shown that damage of certain organs leads to an increase in the tissue-specific miRNA levels in the circulation (Laterza et al, 2009;Corsten et al, 2010;Zhang et al, 2010;Uhlemann et al, 2012;Banzet et al, 2013;Akat et al, 2014). It is feasible, that the majority of these miRNAs is EVfree and associated purely with AGO proteins.…”

MicroRNAs: Marcadores séricos en diabetes mellitus tipo 2 y ejercicio físico

“…Ulheman encontró que el ejercicio de resistencia muscular anaeróbico (cargas excéntricas) generaba incremento de miR-133 (específico de músculo esquelético) 36 . Banzet y cols., también identificaron elevación de miRNAs específicos de músculo esquelético (hsa-miR-1, miR-133a, miR-133b y miR-208b) con ejercicio muscular excéntrico (caminata cuesta abajo), el cual se asocia a daño muscular 37 . En contraste, Sawada y cols., no reportaron incremento de los mio-miRNAs en respuesta a un ejercicio de resistencia muscular anaeróbico (levantamiento de pesas con piernas) 38 (Tabla 1).…”