Changes in concentrations of haemostatic and inflammatory biomarkers in synovial fluid after intra-articular injection of lipopolysaccharide in horses

Andreassen, Stine Mandrup; Vinther, Anne Mette; Nielsen, Søren Saxmose; Andersen, Pia Haubro; Tnibar, Aziz; Kristensen, Annemarie T.; Jacobsen, Stine

doi:10.1186/s12917-017-1089-1

“…Concentrations range from essentially 0 mg/L in healthy individuals to several thousand mg/L in horses with severe inflammation [4]. The consistently low concentrations found in healthy horses and the extreme magnitude of response renders SAA a particularly useful marker of inflammation, as shown in a variety of clinical conditions [5][6][7][8][9][10]. Assays relevant for assessment of SAA horse-side or in the smaller laboratories of general practitioners have been validated [11,12].…”

Section: Introductionmentioning

confidence: 99%

Validation of an equine serum amyloid A assay with an unusually broad working range

Jacobsen

¹

,

Vinther

²

,

Kjelgaard‐Hansen

³

et al. 2019

Preprint

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Background Serum amyloid A (SAA) is a major equine acute phase protein and of great value in detection and monitoring of inflammation. A new immunoturbidometric assay based on monoclonal antibodies (VET-SAA, Eiken Chemical Co., Japan) may be useful for SAA measurements in routine diagnostic laboratories. The aim of the study was to validate the VET-SAA immunoturbidometric assay and use it to measure serum SAA concentrations in a variety of clinical cases. Precision was assessed by intra- and interassay coefficients of variation of repeated measurements of serum pools (low, intermediate, high concentrations of SAA). Accuracy was estimated by linearity under dilution. Detection limit was determined by replicate determinations of ionized water. Measurements were compared to measurements performed in a previously validated SAA assay (LZSAA assay, Eiken Chemical Co., Japan). Subsequently, the VET-SAA assay was used for measuring serum SAA concentrations in horses with and without inflammation. Results Detection limit was 1.2 mg/L. Without modifications, the assay measured SAA concentrations with acceptable reliability in a broad concentration range (0 to > 6000 mg/L). In the 0-3000 mg/L range, the assay demonstrated good precision and accuracy, and concentrations correlated well with those obtained in the LZSAA assay, albeit with a slight systematic bias. Concentrations of SAA assessed in horses with and without inflammation followed the expected pattern, with significantly higher concentrations in horses with systemic inflammation than in healthy horses and horses with non-inflammatory disease. Conclusions The assay was unique in its ability to measure SAA concentrations with acceptable reliability over an extreme concentration range. This is relevant in the equine species, where SAA concentrations may reach very high concentrations.

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“…Concentrations range from essentially 0 mg/L in healthy individuals to several thousand mg/L in horses with severe inflammation [4]. The consistently low concentrations found in healthy horses and the extreme magnitude of response renders SAA a particularly useful marker of inflammation, as shown in a variety of clinical conditions [5][6][7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Validation of an equine serum amyloid A assay with an unusually broad working range

Jacobsen

¹

,

Vinther

²

,

Kjelgaard‐Hansen

³

et al. 2019

Preprint

Self Cite

0

View full text Add to dashboard Cite

Background Serum amyloid A (SAA) is a major equine acute phase protein and of great value in detection and monitoring of inflammation. A new immunoturbidometric assay based on monoclonal antibodies (VET-SAA, Eiken Chemical Co., Japan) may be useful for SAA measurements in routine diagnostic laboratories. The aim of the study was to validate the VET-SAA immunoturbidometric assay and use it to measure serum SAA concentrations in a variety of clinical cases. Precision was assessed by intra- and interassay coefficients of variation of repeated measurements of serum pools (low, intermediate, high concentrations of SAA). Accuracy was estimated by linearity under dilution. Detection limit was determined by replicate determinations of ionized water. Measurements were compared to measurements performed in a previously validated SAA assay (LZSAA assay, Eiken Chemical Co., Japan). Subsequently, the VET-SAA assay was used for measuring serum SAA concentrations in horses with and without inflammation. Results Detection limit was 1.2 mg/L. Without modifications, the assay measured SAA concentrations with acceptable reliability in a broad concentration range (0 to > 6000 mg/L). In the 0-3000 mg/L range, the assay demonstrated good precision and accuracy, and concentrations correlated well with those obtained in the LZSAA assay, albeit with a slight systematic bias. Concentrations of SAA assessed in horses with and without inflammation followed the expected pattern, with significantly higher concentrations in horses with systemic inflammation than in healthy horses and horses with non-inflammatory disease. Conclusions The assay was unique in its ability to measure SAA concentrations with acceptable reliability over an extreme concentration range. This is relevant in the equine species, where SAA concentrations may reach very high concentrations.

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“…No presente estudo, foram observadas apenas quatro articulações FTP (5%) dentro do grupo OC; de acordo com Van Weeren e Denoix (2013), a herdabilidade para o desenvolvimento da OC nas articulações TT, MCF e MTF é moderada, enquanto aparentemente não existe para a articulação FTP A contagem leucocitária média no LS é de até 300 células/µl em articulações hídigas em repouso (MOREIRA et al, 2015;NEUENSCHWANDER, 2016;VENDRUSCOLO, 2017) ou submetidas ao exercício (BACCARIN et al, 2014), valor semelhante ao encontrado no presente estudo. Equinos com sinovite induzida experimentalmente apresentaram aumento de células nucleadas no LS poucas horas após o desafio articular com LPS (ANDREASSEN et al, 2017;NEUENSCHWANDER, 2016). Este comportamento leucocitário corresponde à ativação da hemostasia em resposta ao estimulo inflamatório, uma vez que aumento concomitante das concentrações de fibrinogênio e proteína total também foi observado (ANDREASSEN et al, 2017).…”

Section: Quantificação Da Pge2unclassified

“…Equinos com sinovite induzida experimentalmente apresentaram aumento de células nucleadas no LS poucas horas após o desafio articular com LPS (ANDREASSEN et al, 2017;NEUENSCHWANDER, 2016). Este comportamento leucocitário corresponde à ativação da hemostasia em resposta ao estimulo inflamatório, uma vez que aumento concomitante das concentrações de fibrinogênio e proteína total também foi observado (ANDREASSEN et al, 2017). O uso de anfotericina B na indução da sinovite é descrito por Barrachina et al (2016), que observaram aumento da contagem de células nucleadas até 15 dias após estimulo.…”

Section: Quantificação Da Pge2unclassified

“…Além disso, a lesão física celular estimula a degradação enzimática de proteoglicanos e colágeno, assim como a diminuição da síntese de componentes da MEC(FUNATO et al, 2017;LUCIA et al, 2013;MCILWRAITH, 2005;KAHN et al, 2017).Sinovite e capsulite consistem os problemas mais comuns nas articulações com OA e contribuem para o processo de degradação cartilagínea pela liberação de enzimas, mediadores inflamatórios e citocinas(BOYCE et al, 2013;TE MOLLER;VAN WEEREN, 2017;WANG et al, 2017). A importância da inflamação da membrana sinovial na patogênese da OA equina foi reportada por estudos experimentais(ANDREASSEN et al, 2017;BARRACHINA et al, 2016;VAN SICKLE, 1981; NEUENSCHWANDER, 2016) que a demonstraram que a degradação da cartilagem pode ocorrer na ausência de instabilidade ou traumas teciduais, e que a perda de GAGs está associada à ruptura inicial da superfície cartilagínea e à degradação da MEC. Desde então, vários estudos têm reconhecido e demonstrado a importância da sinovite e capsulite na produção de dor e desconforto nos cavalos, bem como no aumento da produção de mediadores contribuintes para o processo osteoartrítico(BROSSI, 2014;CREMA et al, 2017;MCILWRAITH et al, 2017).Patologias dos meniscos são diretamente proporcionais a idade e estão fortemente correlacionadas com o aparecimento da OA humana(BROPHY et al, 2012;CHU; ANDRIACCHI, 2015) e equina(DUBUC et al, 2018), bem como as disfunções mitocondriais(DESJARDIN et al, 2014), uma vez que a mitocôndria prejudicada desempenha papel fundamental na produção de EROs, envolvidas na morte de condrócitos e digestão da MEC(FUNATO et al, 2017).…”

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Changes in concentrations of haemostatic and inflammatory biomarkers in synovial fluid after intra-articular injection of lipopolysaccharide in horses

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References 77 publications

Validation of an equine serum amyloid A assay with an unusually broad working range

Validation of an equine serum amyloid A assay with an unusually broad working range

Validation of an equine serum amyloid A assay with an unusually broad working range

>b<Avaliação do queratam sulfato e ácido hialurônico presentes no líquido sinovial como biomarcadores das principais enfermidades articulares em equinos>/b<

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