Changes in content of steroid regulators during cold hardening of winter wheat - Steroid physiological/biochemical activity and impact on frost tolerance

Janeczko, Anna; Pociecha, Ewa; Dziurka, Michał; Jurczyk, Barbara; Libik-Konieczny, Marta; Oklešťková, Jana; Novák, Ondřej; Pilarska, Maria; Filek, Maria; Rudolphi-Skórska, Elżbieta; Sadura, Iwona; Siwek, Agata

doi:10.1016/j.plaphy.2019.03.020

Cited by 25 publications

(34 citation statements)

References 61 publications

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“…Further, Li et al [12] in studies using electron paramagnetic resonance observed an increase in the membrane fluidity in the presence of brassinolide in mango, which was an important step for developing an improved plant tolerance to low temperature. Our studies [14,15] showed that the structural properties of the cell membranes were differentiated by the presence of brassinosteroids, and therefore, the role of BR in improving the tolerance of winter wheat to low temperatures was suggested. In Langmuir monolayer studies, 24-epibrassinolide and 24-epicastasterone were introduced into lipids that had been obtained from the aerial part of winter wheat seedlings that had been cultured at 5 • C or 20 • C. It was suggested that the tested BR (similar to sterols) entered the cell membrane directly and modified its properties by, for example, increasing the distance between the fatty acid chains, which might improve the functioning (flexibility) of the membrane in low temperatures [14,15].…”

Section: Introductionmentioning

confidence: 78%

“…Our studies [14,15] showed that the structural properties of the cell membranes were differentiated by the presence of brassinosteroids, and therefore, the role of BR in improving the tolerance of winter wheat to low temperatures was suggested. In Langmuir monolayer studies, 24-epibrassinolide and 24-epicastasterone were introduced into lipids that had been obtained from the aerial part of winter wheat seedlings that had been cultured at 5 • C or 20 • C. It was suggested that the tested BR (similar to sterols) entered the cell membrane directly and modified its properties by, for example, increasing the distance between the fatty acid chains, which might improve the functioning (flexibility) of the membrane in low temperatures [14,15]. As 24-epicastasterone induced a slightly different effect than 24-epibrassinolide, these results also showed the importance of the chemical structure of BR for their interactions with membranes [14].…”

Section: Introductionmentioning

confidence: 78%

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The Impact of Mutations in the HvCPD and HvBRI1 Genes on the Physicochemical Properties of the Membranes from Barley Acclimated to Low/High Temperatures

Rudolphi-Szydło

Sadura

Filek

et al. 2020

Cells

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(1) Background: The study characterized barley mutants with brassinosteroid (BR) biosynthesis and signaling disturbances in terms of the physicochemical/structural properties of membranes to enrich the knowledge about the role of brassinosteroids for lipid metabolism and membrane functioning. (2) Methods: The Langmuir method was used to investigate the properties of the physicochemical membranes. Langmuir monolayers were formed from the lipid fractions isolated from the plants growing at 20 °C and then acclimated at 5 °C or 27 °C. The fatty acid composition of the lipids was estimated using gas chromatography. (3) Results: The BR-biosynthesis and BR-signaling mutants of barley were characterized by a temperature-dependent altered molar percentage of fatty acids (from 14:0 to 20:1) in their galactolipid and phospholipid fractions in comparison to wild-type (WT). For example, the mutants had a lower molar percentage of 18:3 in the phospholipid (PL) fraction. The same regularity was observed at 5 °C. It resulted in altered physicochemical parameters of the membranes (Alim, πcoll, Cs−1). (4) Conclusions: BR may be involved in regulating fatty acid biosynthesis or their transport/incorporation into the cell membranes. Mutants had altered physicochemical parameters of their membranes, compared to the WT, which suggests that BR may have a multidirectional impact on the membrane-dependent physiological processes.

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Section: Introductionmentioning

confidence: 78%

Section: Introductionmentioning

confidence: 78%

The Impact of Mutations in the HvCPD and HvBRI1 Genes on the Physicochemical Properties of the Membranes from Barley Acclimated to Low/High Temperatures

Rudolphi-Szydło

Sadura

Filek

et al. 2020

Cells

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“…According to literature, the content of BRs increased in cold hardened winter rye (Pociecha et al 2016) and spring barley (Sadura et al 2019), which is to some extent in accordance with data obtained in this study as significant increase in HCS content was noted for more freezing tolerant DH lines of barley. Previous studies with spring barley (Sadura et al 2019) and winter wheat (Janeczko et al 2019) also showed that cultivars less tolerant to freezing accumulated lower amounts of BRs (especially HCS) in response to cold hardening in comparison with more tolerant cultivars. These results suggest that although the final content of HCS measured in cold hardened seedlings cannot be used as the marker of freezing tolerance in barley, such role could be played by the amplitude of changes induced by the process of hardening.…”

Section: Changes In Phytohormone Content Associated With Stress Treatmentioning

confidence: 86%

Involvement of homocastasterone, salicylic and abscisic acids in the regulation of drought and freezing tolerance in doubled haploid lines of winter barley

et al. 2019

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Ten doubled haploid (DH) lines of winter barley with an increased range of freezing/drought tolerance were used to identify phytohormones involved in plant stress acclimation. Cold hardening and drought stress were applied at the most critical stages of plant development on young seedlings and heading plants, respectively. The level of the phytohormones was significantly higher at heading, more than 5-fold in respect of salicylic acid (SA) and total brassinosteroids (BRs) and 1.7-fold in the case of abscisic acid (ABA). Moreover, the spectrum of detectable BRs increased from one-homocastasterone (HCS)-found in seedlings to four BRs identified in heading plants [HCS, castasterone (CS), teasterone and dolicholide], with the last one detected for the first time in cereal species. To some extent freezing tolerance seems to be determined by native hormonal status as control seedlings of tolerant DH lines contained 1.4-and 2.3-fold lower amount of ABA and HCS and 2.3-fold higher amount of SA in comparison to freezing-sensitive ones. Such dependency was not observed in heading plants as significant variation in CS content was the only detected difference. Under stress treatments, tolerant DH lines accumulated significantly lower (75-81%) amount of ABA, which probably reflected lower stress intensity resulting from another defence strategy. In contrast, stress-induced significant almost 2-fold increase in HCS/CS and 2-3-fold decrease in SA content specific for tolerant DH lines of barley suggest the involvement of these molecules in freezing/drought defence. Detected correlations suggest their interaction with nonspecific peroxidase and low molecular weight antioxidants.

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“…This is the natural ability of winter plants to survive in winter conditions [2]. Cold acclimation at temperatures that usually are in the range of +1 to +5 • C (often preceded by prehardening) induce biochemical and physiological changes, in the content and composition of carbohydrates, growth regulators, antioxidants, phospholipids, fatty acids, and water content, among others [3][4][5][6][7][8]. Plants with a rosette with seven to eight leaves, an apical bud that was located no higher than 3 cm above the soil surface, a root neck more than 5 mm thick and a pile root more than 15 cm long guarantee a good wintering [9].…”

Section: Introductionmentioning

confidence: 99%

Deacclimation of Winter Oilseed Rape—Insight into Physiological Changes

et al. 2020

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Climate changes, which result in the occurrence of periods with relatively high temperatures during the winter, can lead to the deacclimation of cold-hardened plants and cause problems with their winter survival. The aim of these studies was to investigate the physiological changes (photosynthesis and water relations including aquaporin expression) that accompany deacclimation process in the economically important winter oilseed rape plants. The effect of deacclimation on frost tolerance was also estimated for two tested cultivars: semi-dwarf and plants of a normal height. After cold acclimation at 4 °C (compared to the unacclimated control) the typical changes that accompany cold acclimation such as an increase in the content of water-soluble sugars or a lower water content in the leaves, which lead to an increased frost tolerance, were observed. Deacclimation partially or completely reversed these changes, which resulted in a decreased frost tolerance that was accompanied by a decrease in the content of sugars and an increase of the osmotic potential. The chemical composition of the leaves, which was measured using FT-Raman spectroscopy also clearly confirmed the metabolic differences between the cold-acclimated and deacclimated plants. The plants were significantly different in regard to the content of the various pigments as well as fatty acids and polysaccharides. The phenomenon of a deacclimation-induced decrease in aquaporin PIP1 accumulation, which was accompanied by unchanged PIP1 transcript accumulation, will be discussed in the aspects of the water relations and decreased frost tolerance in deacclimated plants.

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Changes in content of steroid regulators during cold hardening of winter wheat - Steroid physiological/biochemical activity and impact on frost tolerance

Cited by 25 publications

References 61 publications

The Impact of Mutations in the HvCPD and HvBRI1 Genes on the Physicochemical Properties of the Membranes from Barley Acclimated to Low/High Temperatures

The Impact of Mutations in the HvCPD and HvBRI1 Genes on the Physicochemical Properties of the Membranes from Barley Acclimated to Low/High Temperatures

Involvement of homocastasterone, salicylic and abscisic acids in the regulation of drought and freezing tolerance in doubled haploid lines of winter barley

Deacclimation of Winter Oilseed Rape—Insight into Physiological Changes

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