Changes in Cytokine Levels and NK Cell Activation Associated with Influenza

Jost, Stéphanie; Quillay, Héloïse; Reardon, Jeff; Peterson, Eric C.; Simmons, Rachel P.; Parry, Blair A.; Bryant, Nancy N. P.; Binder, William; Altfeld, Marcus

doi:10.1371/journal.pone.0025060

Cited by 63 publications

(59 citation statements)

References 59 publications

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“…Also the intracellular cytokine staining of IFN-γ, TNF, IL-10, IL-13 and IL-17A in CD3 -56 bright and CD3 -56 dim cells respectively, showed no significant differences between patients and controls. Although previous reports showed that expression of activation markers on NK cells are increased in some inflammatory conditions (Evans et al, 2011, Jost et al, 2011 we could not confirm these findings in our study populations; probably the systemic inflammation is too low in CAD or reduced by treatment, to induce these activation markers on NK cells. In contrast, our previous studies showed that NK cells in CAD were more prone to become activated and undergo apoptosis (Jonasson et al, 2005, Li et al, 2008 and also in paper II we found that proportions of NK type 1 cells (IL-18R + ) were increased in ACS compared to SA patients.…”

Section: Nk Subpopulations (Paper Iii)contrasting

confidence: 92%

“…+ cells are few but may be increased in acute inflammatory diseases (Evans et al, 2011, Jost et al, 2011. On the other hand CD69 is frequently more expressed on T cells in LN, suggested to promote lymphocyte retention (Shiow et al, 2006).…”

Section: Identification Of Lymphocyte Subpopulationsmentioning

confidence: 99%

“…It is constitutively expressed on APCs including dendritic cells, monocytes/macrophages and B cells. In blood the numbers of HLA DR + T cells are few but they may be increased in acute and chronic inflammatory diseases (Evans et al, 2011, Jost et al, 2011.…”

Section: Identification Of Lymphocyte Subpopulationsmentioning

confidence: 99%

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T Cells and NK Cells in Coronary Artery Disease : Longitudinal and methodological studies in humans

Backteman¹

2014

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Section: Nk Subpopulations (Paper Iii)contrasting

confidence: 92%

Section: Identification Of Lymphocyte Subpopulationsmentioning

confidence: 99%

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T Cells and NK Cells in Coronary Artery Disease : Longitudinal and methodological studies in humans

Backteman¹

2014

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“…During asymmetric division, parental T cells yield both effector (CD4 − CD8 hi ) and long lived memory (CD4 − CD8 lo ) cytotoxic cells [30–32]. Due to the restricted number of markers labeled in this study, the CD4 − CD8 lo cytotoxic T memory cells could not be distinguished from the NK cells, which bear the CD4 − CD8 lo phenotype and upregulate CD25 as well [33]. …”

Section: Resultsmentioning

confidence: 99%

Protection against henipaviruses in swine requires both, cell-mediated and humoral immune response

Pickering

Hardham

Smith

et al. 2016

Vaccine

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Hendra virus (HeV) and Nipah virus (NiV) are members of the genus Henipavirus, within the family Paramyxoviridae. Nipah virus has caused outbreaks of human disease in Bangladesh, Malaysia, Singapore, India and Philippines, in addition to a large outbreak in swine in Malaysia in 1998/1999. Recently, NiV was suspected to be a causative agent of an outbreak in horses in 2014 in the Philippines, while HeV has caused multiple human and equine outbreaks in Australia since 1994. A swine vaccine able to prevent shedding of infectious virus is of veterinary and human health importance, and correlates of protection against henipavirus infection in swine need to be better understood. In the present study, three groups of animals were employed. Pigs vaccinated with adjuvanted recombinant soluble HeV G protein (sGHEV) and challenged with HeV developed antibody levels considered to be protective prior to the challenge (titers of 320), however activation of the cell-mediated immune response was not detected, and the animals were only partially protected against challenge with 5x105 PFU of HeV per animal. In the second group, cross-neutralizing antibody levels against NiV in the sGHEV vaccinated animals did not reach protective levels, and with no activation of cellular immune memory, these animals were not protected against NiV. Only pigs orally infected with 5x104 PFU of NiV per animal were protected against nasal challenge with 5x105 PFU of NiV per animal. This group of pigs developed protective antibody levels, as well as cell-mediated immune memory. Peripheral blood mononuclear cells restimulated with UV-inactivated NiV upregulated IFN-gamma, IL-10 and the CD25 activation marker on CD4+CD8+ T memory helper cells and to lesser extent on CD4−CD8+ T cells. In conclusion, both humoral and cellular immune responses were required for protection of swine against henipaviruses.

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“…IL-2R signalling activates IKKα which results in the recruitment of IκBα and this stimulates the binding of NFκB components resulting in an enhanced PRF1 expression in NK cells [22]. Relatively low levels of IL-2 are present in the plasma during influenza infection causing a possible reduction in NK related IFN-γ secretion and cytotoxic activity [24]. Reduced cytotoxic activity is a hallmark of CFS/ME and this may promote viral thrive, persistence and suppressed pathogen clearance.…”

Section: Discussionmentioning

confidence: 99%

Enhanced Gene Expression Following Vaccination in Chronic Fatigue Syndrome/Myalgic Encephalomyelitis

Brenu¹,

Atkinson²,

Driel³

et al. 2013

IJCM

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Vaccines have been shown to cause differential expression of genes and increase antibody titers against antigens. Influenza vaccines may have an effect on unexplained disorders such as Chronic Fatigue Syndrome/Myalgic Encephalomyelitis (CFS/ME). Immunological changes have been identified following immunization with trivalent influenza vaccine (TIV). The objective of this pilot study was to examine the consequences of TIV on cytokine and cytotoxic genes in CFS/ME. Peripheral blood mononuclear cells were preferentially isolated from whole blood of 7 CFS/ME patients and 8 controls. Following total RNA extraction and synthesis of cDNA, reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) was used to determine the expression levels of mRNAs for cytotoxic genes (perforin (PRF1), granzyme A (GZMA), granzyme B (GZMB) and cytokine genes. GZMB was significantly increased overall in the CFS/ME patients compared to the controls. GZMA was significantly increased 28 days after vaccination while PRF1 was reduced pre-vaccination but increased 14 days post-vaccination in the CFS/ME patients. There were no significant changes in cytokine genes pre or post vaccination. Administration of TIV may increase the expression of lytic genes in CFS/ME and this may contribute to the increase in cytotoxic activity we observed in these patients post vaccination.

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Changes in Cytokine Levels and NK Cell Activation Associated with Influenza

Cited by 63 publications

References 59 publications

T Cells and NK Cells in Coronary Artery Disease : Longitudinal and methodological studies in humans

T Cells and NK Cells in Coronary Artery Disease : Longitudinal and methodological studies in humans

Protection against henipaviruses in swine requires both, cell-mediated and humoral immune response

Enhanced Gene Expression Following Vaccination in Chronic Fatigue Syndrome/Myalgic Encephalomyelitis

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