Changes in Denitrifier Abundance, Denitrification Gene mRNA Levels, Nitrous Oxide Emissions, and Denitrification in Anoxic Soil Microcosms Amended with Glucose and Plant Residues

Henderson, Sherri L.; Dandie, Catherine E.; Patten, Cheryl L.; Zebarth, Bernie J.; Burton, David L.; Trevors, J. T.; Goyer, Claudia

doi:10.1128/aem.02993-09

Cited by 199 publications

(144 citation statements)

References 41 publications

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“…Numerous studies have examined the factors that influence different pathways involved in denitrification, such as nitrate (Magalhães et al 2005), carbon availability (Henderson et al 2010), pH (Liu et al 2010), and copper concentration (Magalhães et al 2011;Felgate et al 2012). Nevertheless, compaction, as a physical factor, has not received as much attention as the above chemical factors associated with denitrification.…”

Section: Introductionmentioning

confidence: 99%

Compaction stimulates denitrification in an urban park soil using 15N tracing technique

Deng

Rensing

et al. 2013

Environ Sci Pollut Res

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Soils in urban areas are subjected to compaction with accelerating urbanization. The effects of anthropogenic compaction on urban soil denitrification are largely unknown. We conducted a study on an urban park soil to investigate how compaction impacts denitrification. By using 15 N labeling method and acetylene inhibition technique, we performed three coherent incubation experiments to quantify denitrification in compacted soil under both aerobic and anaerobic conditions. Uncompacted soil was set as the control treatment. When monitoring soil incubation without extra substrate, higher nitrous oxide (N 2 O) flux and denitrification enzyme activity were observed in the compacted soil than in the uncompacted soil. In aerobic incubation with the addition of K 15 NO 3 , N 2 O production in the compacted soil reached 10.11 ng N h −1 g −1 as compared to 0.02 ng N h −1 g −1 in the uncompacted soil. Denitrification contributed 96 % of the emitted N 2 O in the compacted soil and 36 % of the emitted N 2 O in the uncompacted soil; total denitrification rate was higher in the compacted soil (up to 79.35 ng N h −1 g −1 ) than in the uncompacted soil (0.11 ng N h −1 g −1 ). Under anaerobic incubation with the addition of K 15 NO 3 , no statistical difference in total N losses and 15 N-(N 2 O+N 2 ) flux between the uncompacted soil and the compacted soil was detected. Compaction promoted soil denitrification and may impact urban N biogeochemical cycling.

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Section: Introductionmentioning

confidence: 99%

Compaction stimulates denitrification in an urban park soil using 15N tracing technique

Deng

Rensing

et al. 2013

Environ Sci Pollut Res

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“…Studies have shown that denitrifying communities are affected by soil conditions including temperature, moisture, pH and substrate (e.g. NO 3 À , organic carbon) concentration (Braker et al, 2010;Henderson et al, 2010;Liu et al, 2010;Stres et al, 2008;Lalisse-Grundmann et al, 1988;Renner and Becker, 1970). High exposures to metals such as cadmium, copper, zinc and silver can also be detrimental to denitrifying communities (Holtan-Hartwig et al, 2002;McKenney and Vriesacker, 1985;Throbäck et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Effect of pyrene on denitrification activity and abundance and composition of denitrifying community in an agricultural soil

Guo

Deng

Qiao

et al. 2011

Environmental Pollution

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a b s t r a c tToxicity of pyrene on the denitrifiers was studied by spiking an agricultural soil with pyrene to a series of concentrations (0e500 mg kg À1 ) followed by doseeresponse and dynamic incubation experiments. Results showed a positive correlation between potential denitrification activity and copy numbers of denitrifying functional genes (nirK, nirS and nosZ), and were both negatively correlated with pyrene concentrations. Based on the comparison of EC 50 values, denitrifiers harboring nirK, nirS or nosZ gene were more sensitive than denitrification activity, and denitrifiers harboring nirS gene were more sensitive than that harboring nirK or nosZ genes. Seven days after spiking with EC 50 concentration of pyrene, denitrifiers diversity decreased and community composition changed in comparison with the control. Phylogenetic analyses of three genes showed that the addition of pyrene increased the proportion of Bradyrhizobiaceae, Rhodospirillales, Burkholderiales and Pseudomonadales. Some species belonging to these groups were reported to be able to degrade PAHs.

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“…Key microbial processes within the soil N cycle are catalyzed by key enzymes, including amoA gene encoding a subunit of ammonia monooxygenase in nitrification, as well as nirS and nirK gene (nitrite reductases) and nosZ gene (nitrous oxide (N 2 O) reductase) involved in denitrification. The diversity and abundance of microorganisms carrying these genes and the actual link to N 2 O emission have been extensively studied in diverse soil ecosystems (for example, Henderson et al, 2010, Leininger et al, 2006Philippot et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Seasonality and resource availability control bacterial and archaeal communities in soils of a temperate beech forest

et al. 2010

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It was hypothesized that seasonality and resource availability altered through tree girdling were major determinants of the phylogenetic composition of the archaeal and bacterial community in a temperate beech forest soil. During a 2-year field experiment, involving girdling of beech trees to intercept the transfer of easily available carbon (C) from the canopy to roots, members of the dominant phylogenetic microbial phyla residing in top soils under girdled versus untreated control trees were monitored at bimonthly intervals through 16S rRNA gene-based terminal restriction fragment length polymorphism profiling and quantitative PCR analysis. Effects on nitrifying and denitrifying groups were assessed by measuring the abundances of nirS and nosZ genes as well as bacterial and archaeal amoA genes. Seasonal dynamics displayed by key phylogenetic and nitrogen (N) cycling functional groups were found to be tightly coupled with seasonal alterations in labile C and N pools as well as with variation in soil temperature and soil moisture. In particular, archaea and acidobacteria were highly responsive to soil nutritional and soil climatic changes associated with seasonality, indicating their high metabolic versatility and capability to adapt to environmental changes. For these phyla, significant interrelations with soil chemical and microbial process data were found suggesting their potential, but poorly described contribution to nitrification or denitrification in temperate forest soils. In conclusion, our extensive approach allowed us to get novel insights into effects of seasonality and resource availability on the microbial community, in particular on hitherto poorly studied bacterial phyla and functional groups.

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Changes in Denitrifier Abundance, Denitrification Gene mRNA Levels, Nitrous Oxide Emissions, and Denitrification in Anoxic Soil Microcosms Amended with Glucose and Plant Residues

Cited by 199 publications

References 41 publications

Compaction stimulates denitrification in an urban park soil using 15N tracing technique

Compaction stimulates denitrification in an urban park soil using 15N tracing technique

Effect of pyrene on denitrification activity and abundance and composition of denitrifying community in an agricultural soil

Seasonality and resource availability control bacterial and archaeal communities in soils of a temperate beech forest

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