Changes in distribution of chloroplast adenylate kinase isoforms during floral induction

Schlattner, Uwe; Wagner, Edgar; Greppin, Hubert; Bonzon, Marc

doi:10.1111/j.1399-3054.1996.tb00220.x

Cited by 2 publications

(3 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In plant cells, AK activity is widely distributed through different compartments, namely plastids, mitochondria, nuclei, and cytosol [22][23][24][25][26]. Different isozymes of leaf AK were purified from various species using affinity chromatography [27].…”

Section: Ak and Ndpk In Cell Energeticsmentioning

confidence: 99%

“…Arabidopsis contains a total of eight genes of AK. Of those, at least two genes code for plastidial AK isozymes [23,25], at least three genes code for mitochondrial AKs [25,28], and one gene codes for nuclear AK [26]. The remaining two genes code for AKs which may be dually targeted between mitochondria and plastids, and mitochondria and cytosol, respectively [25] (Table 1).…”

Section: Ak and Ndpk In Cell Energeticsmentioning

confidence: 99%

“…Upon flower induction, although total AK activity in the leaves and stems remained the same, the intracellular distribution of AK activity changed, with the most prominent being a strong decrease in activity of one of chloroplast AK isozymes. This AK was proposed to functionally interact with the chloroplast adenylate translocator, responding to alterations in energy distribution between chloroplast and cytosol during floral induction [23,102].…”

Section: Adenylate Translocatorsmentioning

confidence: 99%

See 2 more Smart Citations

Magnesium Signaling in Plants

Kleczkowski

Igamberdiev

2021

IJMS

View full text Add to dashboard Cite

Free magnesium (Mg2+) is a signal of the adenylate (ATP+ADP+AMP) status in the cells. It results from the equilibrium of adenylate kinase (AK), which uses Mg-chelated and Mg-free adenylates as substrates in both directions of its reaction. The AK-mediated primary control of intracellular [Mg2+] is finely interwoven with the operation of membrane-bound adenylate- and Mg2+-translocators, which in a given compartment control the supply of free adenylates and Mg2+ for the AK-mediated equilibration. As a result, [Mg2+] itself varies both between and within the compartments, depending on their energetic status and environmental clues. Other key nucleotide-utilizing/producing enzymes (e.g., nucleoside diphosphate kinase) may also be involved in fine-tuning of the intracellular [Mg2+]. Changes in [Mg2+] regulate activities of myriads of Mg-utilizing/requiring enzymes, affecting metabolism under both normal and stress conditions, and impacting photosynthetic performance, respiration, phloem loading and other processes. In compartments controlled by AK equilibrium (cytosol, chloroplasts, mitochondria, nucleus), the intracellular [Mg2+] can be calculated from total adenylate contents, based on the dependence of the apparent equilibrium constant of AK on [Mg2+]. Magnesium signaling, reflecting cellular adenylate status, is likely widespread in all eukaryotic and prokaryotic organisms, due simply to the omnipresent nature of AK and to its involvement in adenylate equilibration.

show abstract

Section: Ak and Ndpk In Cell Energeticsmentioning

confidence: 99%

Section: Ak and Ndpk In Cell Energeticsmentioning

confidence: 99%

Section: Adenylate Translocatorsmentioning

confidence: 99%

See 1 more Smart Citation

Magnesium Signaling in Plants

Kleczkowski

Igamberdiev

2021

IJMS

View full text Add to dashboard Cite

show abstract

Membrane potential, adenylate levels and Mg2+ are interconnected via adenylate kinase equilibrium in plant cells

Igamberdiev

Kleczkowski

2003

Biochimica et Biophysica Acta (BBA) - Bioenergetics

View full text Add to dashboard Cite

Concentrations of adenylate species and free magnesium (Mg(2+)) within cells are mediated by the equilibrium governed by adenylate kinase (AK), the enzyme abundant in plants in chloroplast stroma and intermembrane spaces of chloroplasts and mitochondria. Ratios of free and Mg-bound adenylates (linked to the values of [Mg(2+)] established under AK equilibrium) can be rationalized in terms of the overall dependence of concentrations of Mg(2+) and free and Mg-bound adenylates, as well as electric potential values across the inner membranes of mitochondria and chloroplasts. The potential across the inner mitochondrial membrane, by driving adenylate translocators, equilibrates free adenylates across the inner membrane according to the Nernst equation and contributes to the ATP(total)/ADP(total) ratio in the cytosol. The ratio affects the exchange of free adenylates with chloroplasts and this, in turn, influences the value of potential across the inner chloroplast membrane. From measurements of subcellular ATP(total)/ADP(total) ratios, we suggest a method of estimating the values of potential across inner membranes of mitochondria and chloroplasts in vivo, which allows a comparison of the operation of these organelles under different physiological conditions. We discuss also how the equilibration of adenylates by AK drives adenylate transport across membranes, and establishes [Mg(2+)] in the cytosol and chloroplast stroma, maintaining the rates of photosynthesis and respiration. This provides a tool for metabolomic research, by which the determined concentrations of adenylate species could be used for computation of essential metabolic parameters in the cell and in subcellular compartments.

show abstract

Changes in distribution of chloroplast adenylate kinase isoforms during floral induction

Cited by 2 publications

References 20 publications

Magnesium Signaling in Plants

Magnesium Signaling in Plants

Membrane potential, adenylate levels and Mg2+ are interconnected via adenylate kinase equilibrium in plant cells

Contact Info

Product

Resources

About