Changes in Dpysl2 expression are associated with prenatally stressed rat offspring and susceptibility to schizophrenia in humans

Lee, Hwa Young; Joo, Jaesoon; Nah, Seong‐Su; Kim, Jong Woo; Kim, Hyung‐Ki; Kwon, Jun-Tack; Kim, Young Ock; Kim, Hak-Jae

doi:10.3892/ijmm.2015.2161

Cited by 37 publications

(39 citation statements)

References 43 publications

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“…Interestingly, the Trx1:Crmp2 interaction was mapped to the Cys504 residue of Crmp2, suggesting that Trx1 is an important determinant of Crmp2 posttranslational modifications and function [46]. Although Crmp2 has been primarily associated with neurological diseases including Alzheimer’s and schizophrenia [47–50], Crmp2 is highly expressed in lung tissue as well as lung tumor cells [51]. Although endogenous function of Crmp2 in the lung remains unknown, differential Crmp2 posttranslational modifications are associated with nonsmall cell lung cancer [52].…”

Section: Discussionmentioning

confidence: 99%

A novel mouse model for the identification of thioredoxin-1 protein interactions

Booze

Hansen

Vitiello

2016

Free Radical Biology and Medicine

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Thiol switches are important regulators of cellular signaling and are coordinated by several redox enzyme systems including thioredoxins, peroxiredoxins, and glutathione. Thioredoxin-1 (Trx1), in particular, is an important signaling molecule not only in response to redox perturbations, but also in cellular growth, regulation of gene expression, and apoptosis. The active site of this enzyme is a highly conserved C-G-P-C motif and the redox mechanism of Trx1 is rapid which presents a challenge in determining specific substrates. Numerous in vitro approaches have identified Trx1-dependent thiol switches; however, these findings may not be physiologically relevant and little is known about Trx1 interactions in vivo. In order to identify Trx1 targets in vivo, we generated a transgenic mouse with inducible expression of a mutant Trx1 transgene to stabilize intermolecular disulfides with protein substrates. Expression of the Trx1 “substrate trap” transgene did not interfere with endogenous thioredoxin or glutathione systems in brain, heart, lung, liver, and kidney. Following immunoprecipitation and proteomic analysis, we identified 41 homeostatic Trx1 interactions in perinatal lung, including previously described Trx1 substrates such as members of the peroxiredoxin family and collapsin response mediator protein 2. Using perinatal hyperoxia as a model of oxidative injury, we found 17 oxygen-induced interactions which included several cytoskeletal proteins which may be important to alveolar development. The data herein validates this novel mouse model for identification of tissue- and cell-specific Trx1-dependent pathways that regulate physiological signals in response to redox perturbations.

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Section: Discussionmentioning

confidence: 99%

A novel mouse model for the identification of thioredoxin-1 protein interactions

Booze

Hansen

Vitiello

2016

Free Radical Biology and Medicine

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“…The decrease in the expression of neurofilament and Dpysl2 proteins in the offspring of rats with PNS was affected by VF treatment. Our previous studies have shown that Dpysl2 and neurofilament protein levels decreased in the offspring of rats exposed to PNS (36,37). Furthermore, according to our unpublished data, Lasp1 protein was also downregulated in the PNS group.…”

Section: Discussionmentioning

confidence: 50%

“…All experimental procedures were reviewed and approved by the institutional Review Board for Animal Welfare at Soonchunhyang University. The rat model of PNS was established as described in our previous studies (36,37). Briefly, PNS exposure was initiated on day 14 of gestation and continued until day 21, and consisted of: i) 1 h restraint in a well-ventilated, cylindrical Plexiglas restraint device (Braintree Scientific, Inc. Braintree, MA, USA), ii) 6 h of exposure to a cold environment (4˚C), iii) overnight fasting, iv) 15 min of swimming stress in room-temperature water, v) reversal of the light-dark cycle, and/or vi) social stress induced by overcrowded housing conditions during the dark phase (28,29).…”

Section: Prenatal Stress Procedures Prenatal Stress Proceduresmentioning

confidence: 99%

Effect of Valeriana fauriei extract on the offspring of adult rats exposed to prenatal stress

Lee

Won

et al. 2016

International Journal of Molecular Medicine

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Exposing a pregnant female to stress is a risk factor for the development of psychiatric disorders in the offspring. In the present study, we examined the effects of an extract of Valeriana fauriei (VF) root (100 mg/kg/day, administered on postnatal days 35-56) on behavioral patterns as well as protein expression in the prefrontal cortex of the offspring of prenatally-stressed rats. Modified behavioral tests, including the forced swim test, the open field test, a social interaction test and the prepulse inhibition test were performed and many of the parameters were found to decrease in the offspring of the rats exposed to PNS compared with the offspring of the non-stressed rats. Western blot and immunohistochemical analyses of the prefrontal cortex revealed that the downregulation of several neurodevelopmental proteins in the offspring of rats dams exposed to PNS was reversed after treatment with VF extract. These findings demonstrate that the downregulation of several proteins in the prefrontal cortex of the offspring of prenatally‑stressed rats may be associated with subsequent behavioral changes, and that these phenomena recovered following VF treatment. Our results suggest that VF decreases the incidence of prenatal stress related-psychiatric disorders, such as depression and schizophrenia.

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“…Dpysl2, multifunctional protein of the nervous system, plays a role in neuronal development and polarity, as well as in neuron projection morphogenesis, necessary for signaling-related remodeling of the cytoskeleton (26). Dpysl2 is related to schizophrenia disorder (27). Over-expression of the Dpysl2 causes an increase in the Ca2+ channel density, and CaV2.2 channels have shown a significant increase in vesicular release due to a depolarizing stimulation.…”

Section: Discussionmentioning

confidence: 99%

Proteomic Analysis of Extremely Low-Frequency ElectroMagnetic Field (ELF-EMF) With Different Intensities in Rats Hippocampus

Rezaei‐Tavirani

Hasanzadeh

Seyyedi³

et al. 2018

Arch Neurosci

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Background: Our previous study determined the alteration in behavioral test-related memory and movement in male rats exposed to extremely low frequency electromagnetic field (ELF-EMF). The molecular mechanism of the effect of ELF-EMF on brain damage of rat has not yet fully understood, thus the current study investigated the effect of ELF-EMFs on hippocampus of male rats by the proteomics approach. The aim of this study was to investigate the effects of 50 Hz ELF-EMF at 0.5 and 1 mT 50 Hz for 2 and 4 weeks on male rats and determined protein expression of the hippocampus. Methods: Effects of 0.5 and 1 mT exposures for 2 and 4 weeks on protein expression was determined via proteomics techniques. Statistical analysis of proteome was performed using the Progensis Same Spots software. Bioinformatics analysis based on network was used for determining the mechanism. Results: Two-DE electrophoresis gels determined the effect of ELF-EMF on rat hippocampus, indicating that protein expression was overall down-regulated by increasing ELF-EMF intensity and time. The detected differentially expressed proteins between the groups comprised of Sptan1, Dpysl2, Tpi1, Lap3, Vdac1, and Tppp. Almost all were cytoskeletal proteins, most of which were increased by intensity and time. These proteins attributed to impairment in short memory of hippocampus after exposure to ELF-EMF. The GO analysis based on network, enriched two important biological process, cytoskeletal neurons and metabolic process. Conclusions: This study determined the effect of ELF-EMF, which led to change in protein expression related to the cytoskeleton that contributes to major processes in brain damage.

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Changes in Dpysl2 expression are associated with prenatally stressed rat offspring and susceptibility to schizophrenia in humans

Cited by 37 publications

References 43 publications

A novel mouse model for the identification of thioredoxin-1 protein interactions

A novel mouse model for the identification of thioredoxin-1 protein interactions

Effect of Valeriana fauriei extract on the offspring of adult rats exposed to prenatal stress

Proteomic Analysis of Extremely Low-Frequency ElectroMagnetic Field (ELF-EMF) With Different Intensities in Rats Hippocampus

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