The purposed of this research was to identification effects in the different carotenoid on external and egg yolk quality of Alabio laying ducks (Anas plathyrhyncos borneo). To begin with, the carotenoid was identification using High Performance Liquid Chromatography (HPLC) for lutein content. Second, 200 day-old-Alabio laying ducks by age 4-5 months was used in this study. The basal diet was used in this study T0: basal diet, T1: basal diet + 10% lutein, T2: basal diet + 20% lutein, T3 basal diet + 30% lutein, T4 : basal diet + 40% lutein. A completely randomized design was adjusted with five treatments and six replications for each pen, consisting of ten Alabio laying ducks. A general linear model was used in this research to identification a significant effect. The results showed used different carotenoid give insignificant difference (p < 0.05) on the whole egg quality and yolk quality. In summary, even though present slightly different the carotenoid can be used as natural pigmentation on Alabio laying ducks.