Changes in embryonic development, juvenile growth and physiological adaptation of the cuttlefish Sepia pharaonis in response to photoperiod manipulation

Jiang, Maowang; Chen, Huan; Zhou, Song; Han, Qingxi; Peng, Ruibing; Jiang, Xiamin

doi:10.1007/s00343-021-1243-2

Cited by 5 publications

(2 citation statements)

References 63 publications

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“…S. pharaonis eggs were obtained by artificial breeding. In brief, brood-stock individuals with a male:female ratio close to 1:3 was placed in square concrete tanks (7.8 m × 3.8 m × 1.6 m, length × width × depth; area: 30 m 2 ), at a culture density of 8-10 ind/m 2 during the spawning season from February to April in 2021 (Jiang et al, 2022). These cuttlefish were fed with frozen fishes (Larimichthys polyactis and Pampus argenteus) and white shrimp (Penaeus vannamei) twice a day (fed ad libitum), with a feeding rate of 3-5% body weight (bw) d -1 (Jiang et al, 2018).…”

Section: Animals and Housingmentioning

confidence: 99%

Effects of feed transition on digestive tract digestive enzyme, morphology and intestinal community in cuttlefish (Sepia pharaonis)

Jiang

Xiao

et al. 2022

Front. Mar. Sci.

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Sepia pharaonis is an excellent candidate for aquaculture in China. However, the low survival rate during early feed transition is a bottleneck restricting industrial development. Understanding the changes in digestive physiology and intestinal microflora during feed transition should enable us to meet their nutritional needs to improve production. In this study, we investigate the digestive enzyme of S. pharaonis and undertake histological observations of the digestive gland and intestine. The intestinal microflora 16S rRNA genes were also analyzed using high-throughput sequencing of the pre, mid, and post-feed transition stages (20, 40, and 60 days post-hatching (DPH), respectively). The digestive enzymes from the digestive gland (trypsin and chymotrypsin) rapidly decrease at 40 DPH when compared to their levels at 20 DPH, but mostly recovered by 60 DPH. The alkaline phosphatase and lipase increased sharply by 40 DPH, then peaked at 60 DPH. The intestinal digestive enzymes followed similar trends during feed transition, except for lipase activity, which decreased after 20 DPH and remained low, even at 60 DPH. Feed transition affects the morphogenesis of the digestive tract and feed transition stress leads to the impairment of the digestive gland and intestinal morphology, which reduces the digestive capacity, but almost totally recovers by 60 DPH. Moreover, the comparison of the intestinal microbial composition during feed transition revealed that the dominant phylum Bacteroidetes gradually increased to a peak at 40 DPH and then decreased until 60 DPH. The microbial composition changed with the most abundant genus Pseudomonas being replaced by Acinetobacter. The phylum and family level investigation suggested the microbiota in the rearing water had limited influence on the intestinal microbiota. The intestinal microbiota diversity increased during feed transition. This study improves our understanding of changes and adaptations in cuttlefish during feed transition.

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Section: Animals and Housingmentioning

confidence: 99%

Effects of feed transition on digestive tract digestive enzyme, morphology and intestinal community in cuttlefish (Sepia pharaonis)

Jiang

Xiao

et al. 2022

Front. Mar. Sci.

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“…Individuals can reach a weight of ≤2.2 kg when raised in cement ponds, and thus has become a commercially cultured cephalopod in coastal southeast China (Jiang, Peng, et al, 2019). Recent reports have focused on the development of fertilized eggs and embryos (Lee et al, 2016; Peng et al, 2017), environmental factors for the growth of juveniles (Jiang, Chen, et al, 2022), tissue nutrients, and development of the reproductive system (Gao et al, 2014; Huang et al, 2018; Nair et al, 2005). Given the difficulties in raising S. pharaonis in large‐scale aquaculture, our research group has conducted in‐depth research on the optimal food supply for hatchlings (Peng et al, 2015), the diet conversion strategy in the weaning period (Jiang et al, 2020, 2021), inking physiology (Jiang, Ye, et al, 2022; Jiang, Zhao, et al, 2019), nutritional requirements, and optimizing ecological factors for each growth stage (Jiang, Peng, et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Histological analysis of oogenesis and ovarian development of the pharaoh cuttlefish, Sepia pharaonis

Zhang

Chen

Jiang

et al. 2023

Invertebrate Biology

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The pharaoh cuttlefish, Sepia pharaonis, has become a commercially cultured cephalopod in coastal southeast China. However, information on the reproductive histology of this species remains limited. To describe its reproductive development, this study investigated the ovarian structure and oogenesis of S. pharaonis from hatchlings to the postbreeding stage using anatomical methods and histological techniques. The results showed that oogenesis in the ovary was asynchronous and morphologically variable, with immature oocytes attached to the gonadal cord and encircling the ovarian stroma in sequence. After maturation, the egg was free in the ovarian cavity and was discharged through the fallopian tube to combine with the sperm. Furthermore, serial histological dissections of the ovaries demonstrated that oocyte development was asynchronous. Based on egg size and morphology, follicular cell morphology, and yolk formation, oogenesis in S. pharaonis was divided into five distinct stages: The oogonia stage, protoplasm growth stage, follicular penetration stage, vitellogenesis stage, and resorbing stage. Moreover, based on the appearance of follicular cells in the protoplasm growth stage and their disintegration and disappearance in the vitellogenesis stage, it can be inferred that follicular cells secrete yolk substances and participate in the formation of egg membranes. Through the dynamic observation and description of the ovary development and oogenesis, these results provide an important foundation for studies of the regulatory mechanisms of oogenesis in this species, enriching the theory of cephalopod reproductive biology and improving artificial reproduction technology.

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Embryonic exposure to environmentally relevant levels of tributyltin affects embryonic tributyltin bioaccumulation and the physiological responses of juveniles in cuttlefish (Sepia pharaonis)

Jiang

Zhang

Han

et al. 2023

Ecotoxicology and Environmental Safety

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Changes in embryonic development, juvenile growth and physiological adaptation of the cuttlefish Sepia pharaonis in response to photoperiod manipulation

Cited by 5 publications

References 63 publications

Effects of feed transition on digestive tract digestive enzyme, morphology and intestinal community in cuttlefish (Sepia pharaonis)

Effects of feed transition on digestive tract digestive enzyme, morphology and intestinal community in cuttlefish (Sepia pharaonis)

Histological analysis of oogenesis and ovarian development of the pharaoh cuttlefish, Sepia pharaonis

Embryonic exposure to environmentally relevant levels of tributyltin affects embryonic tributyltin bioaccumulation and the physiological responses of juveniles in cuttlefish (Sepia pharaonis)

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