Changes in Erythrocyte Surface Marker Cd44 During Hypothermic and Low Temperature Storage

Zemlianskykh, N. G.; Babijchuk, L. A.

doi:10.15407/fz62.02.094

Cited by 3 publications

(1 citation statement)

References 22 publications

(25 reference statements)

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“…This assumption is based on the fact that MCC proteins play an important role in maintaining the unique shape of cells and ensuring mechanical stability and deformability of erythrocytes in the bloodstream [5]. In addition, it was previously found that in erythrocytes under the influence of PEG [6] and freeze-thawing processes [7] there is a significant decrease in the expression of the surface marker CD44, which may be induced by disturbances of intermolecular interactions in MCC.…”

Section: Introductionmentioning

confidence: 99%

Modifications of proteins of membrane-cytoskeleton complex and production of reactive oxygen species in erythrocytes cryopreserved with polyethylene glycol

Zemlianskykh¹,

Babiychuk²

2021

Fiziol Zh

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Protein modifications in the membrane-cytoskeleton complex (MCC) of human erythrocytes, as well as changes in the intensity of reactive oxygen species (ROS) production upon cell cryopreservation with polyethylene glycol (PEG) were investigated. The protein profile of ghosts of erythrocytes frozen with PEG has common features with both the control and cells frozen without cryoprotectant. PEG makes it possible to restrict the structural rearrangements of the main MCC proteins under the effect of extreme factors and to restrain the amount of high molecular weight polypeptide complexes induced by the protein-cross-linking reagent diamide at the control level, in contrast to cells frozen without a cryoprotectant. However, changes related to the protein peroxiredoxin 2 in ghosts of erythrocytes cryopreserved with PEG are also attributed to cells frozen without a cryoprotectant that may be associated with the activation of oxidative processes. This is evidenced by a 10-fold increase in ROS formation in erythrocytes frozen under PEG protection. Thus, upon cryopreservation of erythrocytes with PEG, certain disorders in MCC proteins may be associated with increased formation of ROS, which may contribute to the disorganization of the structural components of MCC and disrupt the stability of cryopreserved cells under physiological conditions.

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Section: Introductionmentioning

confidence: 99%

Modifications of proteins of membrane-cytoskeleton complex and production of reactive oxygen species in erythrocytes cryopreserved with polyethylene glycol

Zemlianskykh¹,

Babiychuk²

2021

Fiziol Zh

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Effect of different cryopreservation regimens on Ehrlich carcinoma growth

et al. 2019

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Cold in Biology and Medicine: state of the art and perspectives

Edited by Professor O. Yu. Petrenko

2023

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The monograph highlights the main development stages of the Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine. It provides an infor-mation about the major achievements in cryobiology and cryomedicine, fundamental and applied researches being of a priority value. Certain scientific directions formed during this time and coi-ned as the separate scientific schools have been specially empasized. Particular attention is paid to the basic aspects of cryopreservation of biological objects, mechanisms of cold adaptation of biolo-gical systems, modern methodical approaches to cryopreservation of genetic resources and stem cells of various origins, development of low-temperature techniques in modern biology and regenerative medicine. The monograph is intended to biologists and medical specialists, biology and medicine students, those having an interest in modern cryotechniques in biology and medicine.

show abstract

Changes in Erythrocyte Surface Marker Cd44 During Hypothermic and Low Temperature Storage

Cited by 3 publications

References 22 publications

Modifications of proteins of membrane-cytoskeleton complex and production of reactive oxygen species in erythrocytes cryopreserved with polyethylene glycol

Modifications of proteins of membrane-cytoskeleton complex and production of reactive oxygen species in erythrocytes cryopreserved with polyethylene glycol

Effect of different cryopreservation regimens on Ehrlich carcinoma growth

Cold in Biology and Medicine: state of the art and perspectives

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