Changes in essential oil yield and fatty acid contents in black cumin (Nigella sativa L.) genotypes in response to drought stress

Bayati, Pedram; Karimmojeni, Hassan; Razmjoo, Jamshid

doi:10.1016/j.indcrop.2020.112764

Cited by 42 publications

(17 citation statements)

References 28 publications

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“…Indeed, secondary metabolite synthesis can be considered as functional and structural stabilization of plants to cope with stressful conditions during growth and development through a signaling pathway ( Erb and Kliebenstein, 2020 ; Hatcher et al, 2020 ). Several studies in different plants such as hypericon ( Hypericum polyanthemum ) ( de Matos Nunes et al, 2014 ), Lamiaceae plants ( Kulak, 2020 ), black cumin ( Nigella sativa L.) ( Bayati et al, 2020 ), Salvia species ( Bidabadi et al, 2020 ), and summer savory ( Satureja hortensis ) ( Miranshahi and Sayyari, 2016 ) demonstrated that accumulation of secondary metabolites had been significantly increased under abiotic stresses, especially under drought stress. Alam et al (2014) showed that essential oils, carotenoids, and polyphenols could be categorized as the most important secondary metabolites under drought stress in Citrus species.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Foliar Application of Melatonin on Changes in Secondary Metabolite Contents in Two Citrus Species Under Drought Stress Conditions

Jafari

Shahsavar

2021

Front. Plant Sci.

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Plant secondary metabolites are compounds that play an important role in plant interactions and defense. Persian lime and Mexican lime as the two most important sour lime varieties with high levels of secondary metabolites, are widely cultivated in tropical and subtropical areas. Melatonin is a pleiotropic molecule that plays a key role in protecting plants against drought stress through regulating the secondary metabolite biosynthesis pathway. This study was performed as a factorial experiment consisting of three factors in a completely randomized design (CRD), including four concentrations of melatonin (0, 50, 100, and 150 μM), three levels of drought stress [100% (control), 75% (moderate stress), and 40% (severe stress) field capacity (FC)], and two Citrus cultivars. The experiment was conducted for 60 days in a greenhouse condition. Based on the results of this study under severe drought stress, melatonin-treated crops had higher total flavonoid and total phenolic contents than the untreated crops. The highest level of essential oils components was observed on 100 μM foliar application of melatonin under severe drought stress in both varieties. The main component of the essential oil was limonene in both Citrus species. Moreover, based on the analysis of the results, hesperidin was the main polyphenol in both varieties. Since the use of melatonin often increases the production of secondary metabolites, this study can be considered as a very effective method for controlling the adverse effects of drought stress in citrus for both industrial and horticultural aims.

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Section: Introductionmentioning

confidence: 99%

The Effect of Foliar Application of Melatonin on Changes in Secondary Metabolite Contents in Two Citrus Species Under Drought Stress Conditions

Jafari

Shahsavar

2021

Front. Plant Sci.

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“…Four fatty acid omega-hydroxylase genes were upregulated in DT; their products are known as signaling molecules that act as mediators of plant defense reactions [104]. In plants, fatty acid omega-hydroxylase is essential for the synthesis of the cuticle [105], and fatty acids have also been found to improve drought tolerance [106]. 3-ketoacyl-CoA synthase encodes the rst component of the fatty acid elongation complex [107] that is responsible for the synthesis of wax precursors and is therefore involved in limiting non-stomatal water loss and responding to drought stress [108].…”

Section: Genes Involved Insecondarymetabolismmentioning

confidence: 99%

Comparative analysis of alfalfa (Medicago sativa L.) seedling transcriptomes reveals genotype-specific drought tolerance mechanisms

Wang

et al. 2021

Plant Physiology and Biochemistry

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BackgroundDrought is one of the main abiotic factors that affect alfalfa yield. The identi cation of genes that control this complex trait can provide important insights for alfalfa breeding. However, little is known about how alfalfa responds and adapts to drought stress, particularly in cultivars of differing drought tolerance. ResultsIn this study, the drought-tolerant cultivar Dryland 'DT' and the drought-sensitive cultivar WL343HQ 'DS' were used to characterize leaf and root physiological responses and transcriptional changes in response to water de cit. Under drought stress, Dryland roots (DTR) showed more differentially expressed genes than WL343HQ roots (DSR), whereas WL343HQ leaves (DSL) showed more differentially expressed genes than Dryland leaves (DTL). Many of these genes were involved in stress-related pathways, carbohydrate metabolism, and lignin and wax biosynthesis, which may have improved the drought tolerance of alfalfa. We also observed that several genes related to ABA metabolism, root elongation, peroxidase activity, cell membrane stability, ubiquitination, and genetic processing responded to drought stress in alfalfa. We highlighted several candidate genes, including sucrose synthase, xylan 1,4-betaxylosidase, primary-amine oxidase, and alcohol-forming fatty acyl-CoA reductase, for future studies on drought stress resistance in alfalfa and other plant species. ConclusionsIn summary, our results reveal the unique drought adaptation and resistance characteristics of two alfalfa genotypes. These ndings, which may be valuable for drought resistance breeding, warrant further gene functional analysis to augment currently available information and to clarify the drought stress regulatory mechanisms of alfalfa and other plants. HighlightsWe assembled leaf and root transcriptomes of drought-tolerant and drought-sensitive alfalfa cultivars under control and drought conditions.Under drought, the drought-tolerant cultivar exhibited more differentially expressed metabolites than the drought-sensitive cultivar.Genes associated with carbon metabolism, amino acid metabolism, hormones, and secondary metabolites were differentially expressed under drought stress.The drought-tolerant cultivar exhibited higher expression of genes encoding sucrose synthase, xylan 1,4-beta-xylosidase, primary-amine oxidase, and alcohol-forming fatty acyl-CoA.

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“…The diversity of chemical compounds in the extracts makes attribution of antimicrobial activity to specific compounds difficult, especially when considering toxicity. Hepatic damage can occur with an aqueous extract [ 14 ] and metabolic changes with a lipid extract [ 15 ]. Consequently, the specific chemical(s) that imparts antimicrobial properties to NS must be separated and purified for exploitation.…”

Section: Introductionmentioning

confidence: 99%

Chemical Composition Related to Antimicrobial Activity of Moroccan Nigella sativa L. Extracts and Isolated Fractions

Tiji

Rokni

Benayad

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Background. Nigella sativa L. (NS) is an aromatic and medicinal plant commonly used in Mediterranean cuisine. Its grains contain a large amount of fixed oil and have many therapeutic virtues and medicinal properties (antioxidant, antidiabetic, antimicrobial, and anticancer). Aim. The aim of this work is to study the antimicrobial activity of Nigella sativa L. extracts and separated fractions on various pathogenic strains and to correlate that with its chemical composition. Methods. Extracts from Moroccan Nigella sativa seeds were extracted using successive organic solvents, and their hexane and acetone extracts were separated by column chromatography. The chemical composition of extracts, fractions, and essential oil was determined by GC-MS and HPLC-DAD. Extracts and fractions were evaluated for antimicrobial activity through disk diffusion against Gram-positive bacteria (Staphylococcus aureus, Bacillus cereus, and Listeria innocua), Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa), and yeast (Candida pelliculosa) for 1 mg/mL concentration. Bacterial strains were followed to study their behaviors over time in different concentrations. The minimum inhibitory concentration of Nigella sativa essential oil was determined against Staphylococcus aureus, Bacillus cereus, Escherichia coli, and Candida albicans. Results and Conclusion. Although hexane extract was active against both types of bacteria (Gram+ and Gram−), some of its fractions were specifically active against only one type. Fraction (SH4) had the highest activity (15 mm inhibitory diameter). Acetone extract was nonactive but surprisingly resulted in specific active fractions, and the most interesting one was (SA7) that had an inhibitory diameter of 13 mm. This antibacterial effect was related to fatty acids (linoleic and palmitic acids) in (SH4) and 17 pentatriacontene in (SA7). Moreover, the antifungal activity of hexane fractions (10–13 mm) was higher than hexane extract (8 mm), but for acetone, it was the opposite. Acetone extract had a higher activity (18 mm) than its fractions (8–12 mm), except for (SA7) (19 mm). Those inhibitions were attributed to gallic acid, cysteine, and apigenin in acetone extract and cysteine with ascorbic acid in fraction (SA7). Antifungal activity of the essential oil was more pronounced than the antibacterial one. Indeed, determined MICs in the first case were on the microgram scale (MIC = 8 μg/mL, Candida albicans), while in the second case, they were on the milligram scale (MIC = 0.96 mg/mL for Staphylococcus aureus, 0.5 mg/mL for Bacillus cereus, and 0.68 mg/mL for Escherichia coli). This antifungal activity was attributed to three major compounds beta-cymene, alpha-thujene, origanene, and thymoquinone. Results of strains behavior over time at different concentrations of the fractions showed all the curves went through a maximum around 20 hours and had a delay of expression of 5 hours at the start. Taking all results into count, Nigella sativa L. extracts and/or derived principles could form promising antimicrobial agents for therapeutical and industrial uses.

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Changes in essential oil yield and fatty acid contents in black cumin (Nigella sativa L.) genotypes in response to drought stress

Cited by 42 publications

References 28 publications

The Effect of Foliar Application of Melatonin on Changes in Secondary Metabolite Contents in Two Citrus Species Under Drought Stress Conditions

The Effect of Foliar Application of Melatonin on Changes in Secondary Metabolite Contents in Two Citrus Species Under Drought Stress Conditions

Comparative analysis of alfalfa (Medicago sativa L.) seedling transcriptomes reveals genotype-specific drought tolerance mechanisms

Chemical Composition Related to Antimicrobial Activity of Moroccan Nigella sativa L. Extracts and Isolated Fractions

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