Changes in Expression Pattern of Selected Endometrial Proteins following Mesenchymal Stem Cells Infusion in Mares with Endometrosis

Mambelli, Lisley I.; Mattos, Rodrigo Costa; Winter, Gustavo Henrique Zimmermann; Madeiro, Dener S.; Morais, Bruna Pereira de; Malschitzky, Eduardo; Miglino, María Angélica; Kerkis, Alexandre; Kerkis, Irina

doi:10.1371/journal.pone.0097889

Cited by 39 publications

(36 citation statements)

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“…Apesar de a endometriose ser uma patologia degenerativa irreversível, existem estudos que avaliam a eficácia de alguns tratamentos para essa afecção uterina (Alvarenga et al, 2016, Mambelli et al, 2014. Um dos tratamentos para endometriose em éguas mais recentemente que tem sido estudado é com o uso de células tronco mesenquimais.…”

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“…Segundo Bader et al (1997), defeitos no epitélio endometrial de éguas causa alterações na expressão de proteínas o qual resulta em distúrbios no microambiente uterino, causando um déficit na nutrição embrionária e perda embrionária precoce. Mambelli et al (2014) isolaram e expandiram in vitro com sucesso células tronco mesenquimais multipotentes de adipócitos equinos (eAT-MSCs) e analisaram a capacidade alogênica dessas células em influenciar a expressão de certas proteínas em tecido endometrial de éguas para promover um positivo remodelamento local. Mambelli et al (2014) colocaram as células eAT-MSCs em cateter e realizaram a infusão intrauterina imediatamente.…”

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“…Mambelli et al (2014) isolaram e expandiram in vitro com sucesso células tronco mesenquimais multipotentes de adipócitos equinos (eAT-MSCs) e analisaram a capacidade alogênica dessas células em influenciar a expressão de certas proteínas em tecido endometrial de éguas para promover um positivo remodelamento local. Mambelli et al (2014) colocaram as células eAT-MSCs em cateter e realizaram a infusão intrauterina imediatamente. Para confirmar o efeito alogênico no tecido endometrial, foi utilizado fluorescência.…”

Section: Tratamentosunclassified

“…Mambelli et al (2014) relataram que o transplante de células tronco mesenquimais foi confirmada a incorporação dessas células por uso de imunofluorecência. Foi observado também que as MSCs migraram para o espaço periglandular e em alguns casos contribuíram significativamente para a melhora do epitélio glandular.…”

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“…Alterações morfológicas no endométrio foram acompanhados por mudanças na expressão das proteínas analisadas em animais tratados, não ocorrendo com éguas do grupo controle que continuaram apresentando expressão atípica de proteínas. Sendo assim, Mambelli et al (2014) concluíram que seus dados evidenciaram os benefícios morfológicos e funcionais do transplante de MSCs no o útero de éguas com endometriose. Mambelli et al (2014) …”

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Methods of diagnosis of endometritis in mares

Rua

Quirino²,

Bartholazzi³

et al. 2016

Pubvet

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RESUMO.A presente revisão tem como objetivo abordar a importância de exames diagnósticos para afecções uterinas como no caso de endometrite e endometriose para que se tenha maior sucesso em programas reprodutivos de equinos. A endometrite é a patologia que leva a maior perda econômica na Equideocultura. Essa afecção uterina pode acometer éguas de qualquer idade e em qualquer categoria reprodutiva. Existem éguas mais susceptíveis e situações as quais também levam a uma maior taxa de ocorrência da patologia. A indústria equina vem crescendo muito e cada vez mais busca soluções de alguns problemas encontrados para que aumente a eficiência na utilização das biotecnologias reprodutivas como inseminação artificial e transferência de embriões. O diagnóstico de endometrite através de exames como a citologia uterina, análise microbiológica de amostras coletadas do útero e principalmente da histopatologia através da biópsia de amostra extraída do endométrio das éguas elucidam algumas dúvidas que se tem quando se trabalha com doadoras de embriões que apresentam baixa taxa de recuperação embrionária assim como na escolha de melhores receptoras de embriões já que as mesmas exercem um dos papeis mais importantes no sucesso dos resultados da transferência de embriões. São abordados também alguns tratamento e perspectivas futuras. Os três métodos diagnósticos citados explicados na presente revisão apresentam boa eficiência, com diferença entre os mesmos sendo utilizados em conjunto aumentam a acurácia dos resultados. Diante do exposto, torna-se indispensável a utilização dos métodos diagnósticos discutidos na rotina reprodutiva de éguas doadoras e receptoras de embriões com objetivo de aumento da eficiência na reprodução de equinos. Palavras chave: equinos, patologia reprodução, útero Methods of diagnosis of endometritis in maresABSTRACT. The aim of this review is discuss about the importance of the diagnostic examination for uterine affections as endometritis and endometrosis in order to improve the results in equine breeding programs. Endometritis is a pathology that leads to a great loss in horses creation. This uterine disease affects mares in all different ages and in all reproductive category. There are mares more susceptible as well as situations that provides increase the occurrence of this pathology. The horse industry is increasing constantly and there are necessity of researches about the best solutions of some reproductive problems in order to increase the reproductive efficiency of mares submitted to reproductive technologies as artificial insemination and embryo transfer. The diagnoses of endometritis through exams as uterine cytology, microbiology examination of intrauterine samples and mainly histopathology examination by uterine biopsy clarify some doubts about embryo donor and recipients problems as low embryo recovery rates and high embryo loss rates. This diagnosis methods aid the recipient mare choice, considering this mares very important in the embryo transfer results. Some treatments are also discussed a...

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Methods of diagnosis of endometritis in mares

Rua

Quirino²,

Bartholazzi³

et al. 2016

Pubvet

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Material Perspective on the Structural Design of Artificial Meat

Zhao

Jin

et al. 2021

Advanced Sustainable Systems

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products are receiving increasing attention in the market and academia, but many challenges are still being tackled. [3a,5] Among them, the poor texture resemblance is a major limitation to the development of artificial meat. The existing plant-based meat alternatives products usually develop a mushy texture during chewing due to the lack of structure that animal meat holds. [4] Thus, many studies focus on controlling structures breakdown of the non-meat proteins when chewing. Furthermore, it is generally believed that muscle fibers consisting of bundles of thin filaments give meat products their characteristic texture. [4,6] The available production techniques and strategies for converting non-animal proteins into meat-like fibers have been extensively discussed by Goot and Xiong. [3a,6] However, the skeletal muscle comprises both muscle fibers and surrounding intramuscular connective tissues (Figure 1), which indicates that simply creating a fibrous structure is not enough to mimic animal meat. [7] The connective tissues, an extracellular matrix consisting mainly of collagen, form a thin sheet around the muscle fibers and hold them in hierarchical bundles within the muscles. [8] Anatomically, individual muscle is usually attached to the skeleton or bone by the epimysium that is the outermost layer of connective tissue. Within this connective tissue sheath, a group of muscle fiber bundles is surrounded by the perimysium. Each muscle fiber is consisted of myofibrils and is separated by the endomysium, which is directly attached to the muscle cell membrane. The epimysium, perimysium, and endomysium are hierarchically arranged in complex network architecture and support the morphology of muscles and their locomotion. [9] Also, the contraction and disaggregation of collagenous networks are responsible for the meat texture during meat processing. [7] Thus, defects in the texture of artificial meat may be ascribed to the lack of integrated skeletal muscle structures containing the connective tissue and fibers, resulting in an insufficient supporting capacity. Moreover, the integrated skeletal muscle structures significantly affect the meat quality, including sensory experience and nutritional benefits. [10] It is essential to manufacture artificial meat with desired structures to improve supporting capacity, especially for whole-muscle artificial meat products.Previous researches in materials science have already reported that porous or network structures like the architectural skeleton, tissue engineering scaffold, or metal-organic Artificial meat is a promising solution to the negative impacts of meat consumption on natural resources, public health, and animal welfare. Inspired by the use of framework, scaffold, and skeleton in material science, the concept of supporting structures is introduced into the artificial meat to complement the existing theory of structural design. The available structures in artificial meat are evaluated in prefabrication and post-build strategies to illustrate the relationship between...

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Mesenchymal stem cells alter the frequency and cytokine profile of natural killer cells in abortion‐prone mice

Kahmini

Shahgaldi

Moazzeni

2020

Journal Cellular Physiology

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Natural killer cells, which play a pivotal role in the establishment and maintenance of normal pregnancy, are the most abundant leukocytes at the fetomaternal interface that their subsets frequencies and cytokine profile are influential factors in the preservation of the decidual tolerogenic microenvironment. Any imbalance in NK cells' frequency and functions could be associated with pregnancy failure.Mesenchymal stem cells (MSCs) are shown to have immunomodulatory effects on NK cells and their cytokine profile. The purpose of this study is to evaluate the impact of MSCs therapy on the cytokine profiles and subpopulations of NK cells in a murine model of recurrent pregnancy loss. Adipose-derived MSCs were injected intraperitoneally to the abortion-prone mice on Day 4.5 of gestation. The abortion rate was determined after MSCs administration and the frequency and cytokine profiles of the different subsets of NK cells were determined using the flow cytometry. Our results showed that, in abortion-prone mice, the frequency of CD49b + NK cells was significantly higher than normal pregnant mice that decreased after therapy. We also demonstrated that MSCs downregulated the production of IFN-γ and upregulated IL-4 and IL-10 production by uNK cells. These findings indicate that MSCs can decrease the infiltration of CD49b + NK cells to the fetomaternal interface and modulate the cytokine profile of NK cells from inflammatory to tolerogenic profile and thereby improve the tolerogenic microenvironment at the fetomaternal interface in benefit of pregnancy maintenance.

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Changes in Expression Pattern of Selected Endometrial Proteins following Mesenchymal Stem Cells Infusion in Mares with Endometrosis

Cited by 39 publications

References 25 publications

Methods of diagnosis of endometritis in mares

Methods of diagnosis of endometritis in mares

Material Perspective on the Structural Design of Artificial Meat

Mesenchymal stem cells alter the frequency and cytokine profile of natural killer cells in abortion‐prone mice

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