Changes in fat content and some characteristics of lipolytic activity during pregnancy and lactation in mouse mammary gland.

Anzai, Tadashi; Muto, Kinuko; Komine, Senichi

doi:10.1507/endocrj1954.26.371

Cited by 2 publications

(2 citation statements)

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“…Many of these findings related to the transition from pregnancy to lactation were made decades ago either through ultrastructural analysis [35] or via enzymatic assays [40, 41]. Shortly after parturition adipocytes within the mammary glands of mice start to become depleted so that they present as variably-sized multilocular fat cells along with smaller unilocular adipocytes [35].…”

Section: Introductionmentioning

confidence: 99%

Diverse and Active Roles for Adipocytes During Mammary Gland Growth and Function

Hovey

Aimo

2010

J Mammary Gland Biol Neoplasia

155

139

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The mammary gland is unique in its requirement to develop in close association with a depot of adipose tissue that is commonly referred to as the mammary fat pad. As discussed throughout this issue, the mammary fat pad represents a complex stromal microenvironment that includes a variety of cell types. In this article we focus on adipocytes as local regulators of epithelial cell growth and their function during lactation. Several important considerations arise from such a discussion. There is a clear and close interrelationship between different stromal tissue types within the mammary fat pad and its adipocytes. Furthermore, these relationships are both stage- and species-dependent, although many questions remain unanswered regarding their roles in these different states. Several lines of evidence also suggest that adipocytes within the mammary fat pad may function differently from those in other fat depots. Finally, past and future technologies present a variety of opportunities to model these complexities in order to more precisely delineate the many potential functions of adipocytes within the mammary glands. A thorough understanding of the role for this cell type in the mammary glands could present numerous opportunities to modify both breast cancer risk and lactation performance.

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Section: Introductionmentioning

confidence: 99%

Diverse and Active Roles for Adipocytes During Mammary Gland Growth and Function

Hovey

Aimo

2010

J Mammary Gland Biol Neoplasia

155

139

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show abstract

“…Thus, the mitochondria from liver and mammary gland either have a differential sensitivity to NEFA or the concentrations of NEFA are higher in mitochondria prepared from mammary gland than in those prepared from liver. Studies in lactating mice and guinea pigs suggest that mammary tissue NEFA concentrations could range from 2 to 10 μmol/g of wet weight (Kuhn, 1967;Anzai et al, 1979). In addition, analysis of lipid synthesis in the lactating mouse has demonstrated that tissue lipid concentrations may be up to 4-fold higher in the mammary gland compared with the liver (Smith et al, 1969).…”

mentioning

confidence: 99%

Technical note: Assessing the functional capacity of mitochondria isolated from lactating mammary tissue: Choose your chelating agent wisely

Hadsell

George

Abraham

et al. 2009

Journal of Dairy Science

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Previous work has indicated that respiratory activity of mitochondrial preparations prepared from lactating mammary tissue is often much lower than that of mitochondria isolated from other organs such as the liver. Initial studies in our own laboratory also found that mammary mitochondria prepared from lactating mice had much lower ATP synthesis activity than those isolated from liver tissue obtained from the same animals. In this paper, we describe an improved procedure for obtaining coupled mitochondria from the mammary tissue of lactating mice. Using a high-throughput assay for mitochondrial ATP synthesis, we demonstrated that mammary mitochondria, unlike liver mitochondria, are sensitive to the concentration of bovine serum albumin and to the choice of chelating agent used in the preparation and assay buffers. Mammary mitochondria prepared and assayed in buffers containing 1 mM ethylene glycol-bis-(beta-aminoethyl ether)-N,N' tetraacetic acid (EGTA) and 0.4% bovine serum albumin have a similar ATP synthesis activity as liver mitochondria. In addition, we show that the chelating agent EDTA ablates the ATP synthesis capacity of mammary mitochondria through a mechanism that does not involve the release of cytochrome c. We also demonstrate that these improved isolation and assay procedures are both scalable and applicable to bovine mammary tissue, and we describe optimal conditions for cryopreservation and recovery of functionally active mitochondria. This work will facilitate future studies aimed at determining the importance of mammary mitochondria to milk production.

show abstract

Changes in fat content and some characteristics of lipolytic activity during pregnancy and lactation in mouse mammary gland.

Cited by 2 publications

References 4 publications

Diverse and Active Roles for Adipocytes During Mammary Gland Growth and Function

Diverse and Active Roles for Adipocytes During Mammary Gland Growth and Function

Technical note: Assessing the functional capacity of mitochondria isolated from lactating mammary tissue: Choose your chelating agent wisely

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