Changes in Follicular Helper T Cells in Idiopathic Thrombocytopenic Purpura Patients

Xie, Jue; Liu, Yan; Jin, Jie; Tong, Hongyan; Wang, Lei; Ruan, Guangfeng; Lu, Yun; Yuan, Huiming

doi:10.7150/ijbs.10178

Cited by 41 publications

(36 citation statements)

References 43 publications

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“…Interestingly, these are autoantibody-mediated autoimmune diseases. Furthermore, studies have showed both an expansion of cT FH cells and an association with the autoantibody titers in these autoimmune diseases [32][33][34]. In the present study, CVID patients with autoimmune diseases showed increased, but not significant, proportion of cT FH cells.…”

Section: Discussionsupporting

confidence: 46%

Phenotypic and Functional Analysis of T Follicular Cells in Common Variable Immunodeficiency

Kasahara

Bento

Gupta

2020

Int Arch Allergy Immunol

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Introduction: One of the most frequent abnormalities of B cells in common variable immunodeficiency (CVID) is reduced number of class-switched memory B cells, suggesting an impaired germinal center response. Therefore, due to its pivotal role in regulating the development of humoral immunity, the objective of this study was to evaluate the role of circulating T follicular helper (cT FH) and circulating T follicular regulatory (cT FR) cells in the pathogenesis of CVID. Methods: cT FH and cT FR cells from CVID patients and healthy subjects were phenotypically characterized by flow cytometry. cT FH and memory B cells from CVID patients and healthy subjects were isolated and cocultured. Results: Our results showed a reduced proportion of cT FH 17 cells in patients with CVID and an increased ratio of cT FH /cT FR cells in CVID patients with autoimmune diseases. Furthermore, the proportion of IL-21-producing cT FH cells was directly related to the proportion of CD27 + IgD − B cells. Interestingly, coculture assay showed that CVID-derived cT FH cells are able to help memory B cells from healthy controls to produce immunoglobulins. Conclusions: The proportions of cT FH 17 and cT FR cells are altered in CVID patients; however, the cT FH function in assisting B cells to produce antibodies in vitro is preserved.

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Section: Discussionsupporting

confidence: 46%

Phenotypic and Functional Analysis of T Follicular Cells in Common Variable Immunodeficiency

Kasahara

Bento

Gupta

2020

Int Arch Allergy Immunol

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“…Tfh cells have been identified by phenotypic profile, co‐expressions of the chemokine receptor CXCR5, costimulatory molecules ICOS and programmed cell death protein‐1 (PD‐1) . Several reports consider dysfunctional Tfh cells may play an important role in the pathophysiology of autoimmune disease, such as rheumatoid arthritis, autoimmune thyroid diseases and immunothrombocytopenia .…”

Section: Introductionmentioning

confidence: 99%

The Role of Circulating T Follicular Helper Cells and Regulatory Cells in Non‐Small Cell Lung Cancer Patients

Guo

Heng

et al. 2017

Scand J Immunol

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T follicular helper (Tfh) cells and T follicular regulatory (Tfr) cells are identified as the new subset of immune cells. This study aims to investigate the role of circulating Tfh cells (cTfh) and Tfr (cTfr) cells in the pathogenesis of non-small cell lung cancer (NSCLC). A total of 27 NSCLC patients and 19 age and sex-matched healthy controls were enrolled. The percentage of cTfh and cTfr was detected by flow cytometric analysis. Compared to healthy controls, a significantly higher percentage of both cTfh and cTfr cells were observed in NSCLC patients (for cTfh, 18.88% ± 16.84% versus 5.98% ± 3.70%, P < 0.01; for cTfr, 2.67% ± 2.20% versus 1.14% ± 0.76%, P < 0.01). Furthermore, there was a positive correlation between cTfh/cTfr ratio and age in NSCLC patients (P < 0.05). When taking age 60 as a cut-off, the percentage of both cTfh cells and cTfr cells were higher in older patients than younger patients. Moreover, our data showed there was lower percentage of cTfh cells in NSCLC patients with early stage disease (I and II) (12.10% ± 12.22%) than that in advanced stage disease (III and IV) (30.41% ± 17.87%) (P < 0.01). However, no significant relationship was observed between cTfr cells and clinical stage in NSCLC patients. A higher percentage of cTfh cells was observed in patients with squamous cell carcinoma compared with adenocarcinoma (31.70% ± 20.73% versus. 13.48% ± 11.78%, P < 0.05). Taken together, there was significantly higher percentage of cTfh and cTfr cells in NSCLC patients. cTfh and cTfr cells might play an important role in the pathogenesis of NSCLC patients.

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“…Tfh subset, which regulates B-cell responses and stimulates antibody production, is increased in ITP patients [8]. Splenic Tfh expansion participates in B-cell differentiation and antiplateletantibody production during ITP by IL-21 secretion and CD40/ CD40 L interaction [22].…”

Section: Discussionmentioning

confidence: 99%

“…However, novel approaches of platelet destruction have been identified in ITP (e.g., direct lysis of platelet by CTLs [4] and hepatic clearance of desialylated platelets) [5]. Many cellular mechanisms of immune modulation have been described in patients with ITP, such as increased number of Th1 [6], Th17 [7], and Tfh [8], and decreased number or defective suppressive function of Treg [9].…”

Section: Introductionmentioning

confidence: 99%

Icaritin Improves Antibody-Induced Thrombocytopenia in a Mouse Model by Regulating T-cell Polarization

et al. 2017

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Previous studies have shown that icaritin (ICT) has significant protective effects on immune thrombocytopenia (ITP), and the present study aimed to discuss the mechanism of this protective effect from the aspect of regulating T-cell polarization by an antibody-induced ITP mice model. Mice were given rat anti-mouse CD41 antibody (MWReg30) by intraperitoneal injection for 7 d to produce ITP model. At the same time, ICT was administrated at 10 mg/kg/d orally for 9 d. Peripheral blood platelets were counted by hematology analyzer. Spleen index was also tested. Spleen T-helper cell (Th), cytotoxic T-cell (CTL), Th1, Th2, Th17, regulatory T-cell (Treg), and follicular helper T-cell (Tfh) were quantified by flow cytometry. Serum Th1/Th2/Th17 cytokines were tested by mouse Th1/Th2/Th17 cytometric bead array (CBA) kit and transforming growth factor beta (TGF- were analyzed by enzyme-linked immunosorbent assay (ELISA) kit. The results indicated that ICT (10 mg/kg) protected against MWReg30-induced ITP, as evidenced by increased blood platelets and decreased spleen index. In addition, the imbalance of Th/CTL in ITP mice spleen was regulated by ICT. Meanwhile, ICT inhibited Th1, Th17, and Tfh and improved Th2 and Treg in ITP mice spleen. Furthermore, the results of CBA and ELISA suggested that ICT decreased serum Th1- and Th17-related cytokines and increased Th2 cytokines, as well as promoted the release of TGF-. These results demonstrated that the protective effect of ICT on ITP was mediated by regulating T-cell polarization.

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Changes in Follicular Helper T Cells in Idiopathic Thrombocytopenic Purpura Patients

Cited by 41 publications

References 43 publications

Phenotypic and Functional Analysis of T Follicular Cells in Common Variable Immunodeficiency

Phenotypic and Functional Analysis of T Follicular Cells in Common Variable Immunodeficiency

The Role of Circulating T Follicular Helper Cells and Regulatory Cells in Non‐Small Cell Lung Cancer Patients

Icaritin Improves Antibody-Induced Thrombocytopenia in a Mouse Model by Regulating T-cell Polarization

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