Changes in gas composition and acid‐base values of venous blood samples stored under different conditions in 4 domestic species

Abstract

The results of this study underline the need for rapid assessment of acid-base samples, because any delay, even for 1 hour, may affect the results.

Effects of collecting blood into plastic heparinised Vacutainer®tubes and storage conditions on blood gas analysis values in horses

“…Statistically significant differences were not observed for blood pH in any of the samples analysed during this study (Fig 1; Table 1). This finding was similar to that in other studies (Knowles et al 2006;Picandet et al 2007;Piccione et al 2007) and partially reflects the excellent buffering capacity of blood. Although a statistically significant effect of sampling method or storage temperature on blood pH was not detected (Table 1, Fig 1), the main effect of time was significant when adjusted for sampling method and storage temperature.…”

“…Extensive research has been conducted on the use of plastic syringes for collection and storage of blood for gas analysis (Mahoney et al 1991;Muller-Plathe and Heyduck 1992;Wu et al 1997;Deane et al 2004;Knowles et al 2006;Picandet et al 2007;Piccione et al 2007;Rezende et al 2007). The results of these studies have led to recommendations that plastic syringes with low gas permeability can be used for blood gas analysis as long as blood samples are stored at room temperature and analysed within 30 min of collection.…”

“…The results of these studies have led to recommendations that plastic syringes with low gas permeability can be used for blood gas analysis as long as blood samples are stored at room temperature and analysed within 30 min of collection. Blood gas analysis should not be performed on blood samples collected in plastic syringes and stored in iced water because such storage facilitates oxygen diffusion from the atmosphere across the semi-permeable plastic barrel of the syringe into the blood sample, thereby increasing PO 2 (Mahoney et al 1991;Piccione et al 2007;Rezende et al 2007;Anon 2009).…”

Evaluating the effect of sample type on American alligator (Alligator mississippiensis) analyte values in a point-of-care blood analyser

“…The results in the present study suggest that lactate can be measured reliably in American alligator whole blood and in both fresh and banked frozen plasma samples using the i-STAT analyser. Although the blood gas and acid–base values included in the CG4+ cartridge are susceptible to the effects of processing time, sample handling procedures and storage temperatures ( Piccione et al , 2007 ; Abbott Point of Care Inc., 2013d ), our results provide support for using fresh and frozen plasma samples in an i-STAT device to determine multiple CG4+ cartridge analytes in American alligators. However, it is important to emphasize that the i-STAT was originally intended for use with mammals; therefore, caution must be employed when using the device in other non-mammalian species, and further validation is needed.…”

Effect of syringe type, storage temperature and time delay on venous blood gas values in newborn calves

“…Unless a suitable hand-held machine is available, determination of the latter in food animal practice is usually not feasible in the field, and blood samples must be transported to a laboratory. In contrast to blood samples destined for haematological or biochemical evaluation, which yield reliable results within a sufficient storage period (Bleul et al 2002;Blincoe and Marble 1985), blood-gas variables change quickly depending on storage temperature and may render clinically useless measurements (Piccione et al 2007). The maximum length of time blood samples can be stored for blood gas analysis without affecting values in cattle is controversial; recommendations vary from 1 hour to 1 day (Cingi et al 2009;Gokce et al 2004b;Szenci and Besser 1990).…”