Changes in grapevine DNA methylation and polyphenols content induced by solar ultraviolet-B radiation, water deficit and abscisic acid spray treatments

Marfil, Carlos Federico; Ibañez, Verónica Noé; Alonso, Rodrigo; Varela, A. M.; Bottini, Rubén; Masuelli, Ricardo Williams; Fontana, A.; Berli, Federico

doi:10.1016/j.plaphy.2018.12.021

Cited by 42 publications

(38 citation statements)

References 51 publications

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“…Increasing evidence demonstrates that plant DNA methylation and demethylation are closely associated with various environmental stresses, including cold [7], drought [8], heat [9], heavy metal [10], salt [11], and ultraviolet stresses [12]. Additionally, DNA methylation and demethylation also play an indispensable role in regulating fruit development and ripening [3,13,14], as well as secondary metabolism [15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Genome-wide identification and analysis of DNA methyltransferase and demethylase gene families in Dendrobium officinale reveal their potential functions in polysaccharide accumulation

Silva²,

Li³

et al. 2021

BMC Plant Biol

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Background DNA methylation is a conserved and important epigenetic modification involved in the regulation of numerous biological processes, including plant development, secondary metabolism, and response to stresses. However, no information is available regarding the identification of cytosine-5 DNA methyltransferase (C5-MTase) and DNA demethylase (dMTase) genes in the orchid Dendrobium officinale. Results In this study, we performed a genome-wide analysis of DoC5-MTase and DodMTase gene families in D. officinale. Integrated analysis of conserved motifs, gene structures and phylogenetic analysis showed that eight DoC5-MTases were divided into four subfamilies (DoCMT, DoDNMT, DoDRM, DoMET) while three DodMTases were divided into two subfamilies (DoDML3, DoROS1). Multiple cis-acting elements, especially stress-responsive and hormone-responsive ones, were found in the promoter region of DoC5-MTase and DodMTase genes. Furthermore, we investigated the expression profiles of DoC5-MTase and DodMTase in 10 different tissues, as well as their transcript abundance under abiotic stresses (cold and drought) and at the seedling stage, in protocorm-like bodies, shoots, and plantlets. Interestingly, most DoC5-MTases were downregulated whereas DodMTases were upregulated by cold stress. At the seedling stage, DoC5-MTase expression decreased as growth proceeded, but DodMTase expression increased. Conclusions These results provide a basis for elucidating the role of DoC5-MTase and DodMTase in secondary metabolite production and responses to abiotic stresses in D. officinale.

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Section: Introductionmentioning

confidence: 99%

Genome-wide identification and analysis of DNA methyltransferase and demethylase gene families in Dendrobium officinale reveal their potential functions in polysaccharide accumulation

Silva²,

Li³

et al. 2021

BMC Plant Biol

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“…Although the effects of UV on leaf physiology have been studied, the research performed on berries is more meaningful for agricultural applications. Studies of this type have assessed the effects of both ambient solar and artificially enhanced UV levels using specific cut‐off filters or lamps providing supplemental UV, respectively. In addition, the effects of UV natural gradients have been evaluated .…”

Section: Introductionmentioning

confidence: 99%

Phenolic characteristics acquired by berry skins of Vitis vinifera cv. Tempranillo in response to close‐to‐ambient solar ultraviolet radiation are mostly reflected in the resulting wines

et al. 2019

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BACKGROUND: It is widely recognized that ambient levels of solar ultraviolet (UV) radiation strongly influence the phenolic composition of grape skins. However, it is unknown to what extent this influence is reflected in the resulting wines. RESULTS: Tempranillo grapevines were exposed or non-exposed to close-to-ambient solar UV levels using appropriate filters, and the phenolic profiles and antioxidant capacity of both grape skins and the resulting wines were analyzed. In total, 47 phenolic compounds were identified in skins and wines, including flavonols, anthocyanins, flavanols, stilbenes, and hydroxycinnamic and hydroxybenzoic acids. In UV-exposed grape skins, flavonols and anthocyanins increased, whereas flavanols and hydroxybenzoic acids showed no significant change. These characteristics were conserved in the resulting wines. However, for stilbenes, hydroxycinnamic acids and antioxidant capacity, the effect of UV on grape skins was not conserved in wines, probably as a result of changes during winemaking. In addition, color intensity, total phenols and total polyphenol index of wines elaborated from UV-exposed grapes increased (although non-significantly) compared to those made from non-UV-exposed grapes. CONCLUSION:The phenolic composition of grape skins exposed to close-to-ambient solar UV could predict, to some extent, the phenolic composition of the resulting wines, particularly regarding higher contents of flavonols and anthocyanins. Thus, manipulating the UV radiation received by grape skins could improve wine quality by positively influencing color stability and healthy properties. To our knowledge, this is the first study in which the effects of solar UV radiation on phenolic composition have been assessed from grape skins to wine. Enological parameters of winesNo enological parameter showed significant differences between FUV+ and FUV-samples (Table 2). However, there was a trend for higher values of color intensity, TPI and total phenols in FUV+ than FUV-samples. Responses of other enological parameters of winesAlthough close-to-ambient levels of solar UV radiation greatly influenced the phenolic composition of grape skins and the resulting wines, they did not affect significantly other enological parameters of these wines, such as alcohol content, pH, total acidity, malic acid, color intensity, TPI and total phenols. However, there was a trend for higher values of the three last variables in FUV+ than FUV-samples, in line with the significant results found in wine that J Sci Food Agric 2020; 100: 401-409

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“…Ultraviolet radiation could cause multiple damage to plants. UV-B changed the degree of DNA methylation [ 36 ], and UV-C energy reacted photochemically with DNA and caused damage to the DNA double-strand structure [ 37 ], which would make it almost impossible for injured cells to carry out DNA replication. Continuous UV-C irradiation also caused an overproduction of reactive oxygen species (ROS) in plants, causing peroxidation of cell membranes, cell structure damage, and functional destruction as well as stimulating the antioxidant enzyme system [ 13 ].…”

Section: Discussionmentioning

confidence: 99%

“…Stilbene biosynthesis could be promoted by MYB14 under UV-C [ 43 ]. UV-B can affect low molecular weight polyphenol (LMWP) accumulation by promoting DNA methylation to regulate the acclimation mechanisms of plants [ 36 ]. In this study, UV-C activated the biosynthesis and accumulation of flavonoids, which improved their nutritional value and the tolerance of the berries to stress.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic and Metabolomic Basis of Short- and Long-Term Post-Harvest UV-C Application in Regulating Grape Berry Quality Development

Zhang

et al. 2021

Foods

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In this study, ultraviolet-C (UV-C) was utilized to improve the quality of post-harvest grape berries, and the transcriptomic and metabolomic basis of this improvement was elucidated. Berries of the red grape variety ‘Zicui’ and the white variety ‘Xiangfei’ were chosen to evaluate the effect of short- and long-term UV-C irradiation. Post-harvest UV-C application promoted malondialdehyde (MDA) and proline accumulation, and reduced the soluble solid content in berries. Both the variety and duration of irradiation could modulate the transcriptomic and metabolomic responses of berries to UV-C. Compared with the control, the differentially expressed genes (DEGs) identified under UV-C treatment were enriched in pathways related to metabolite accumulation, hormone biosynthesis and signal transduction, and reactive oxygen species (ROS) homeostasis. Flavonoid biosynthesis and biosynthesis of other secondary metabolites were the shared pathways enriched with differential metabolites. After long-term UV-C irradiation, cis-resveratrol accumulated in the berries of the two varieties, while the differential chalcone, dihydroflavone, flavonoid, flavanol, and tannin components primarily accumulated in ‘Xiangfei’, and some flavonols and anthocyanins primarily accumulated in ‘Zicui’. Based on an exhaustive survey, we made a summary for the effect of UV-C in regulating the quality development of post-harvest grape berries. The results of this study may help to elucidate the mechanism by which UV-C functions and support its efficient application.

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Changes in grapevine DNA methylation and polyphenols content induced by solar ultraviolet-B radiation, water deficit and abscisic acid spray treatments

Cited by 42 publications

References 51 publications

Genome-wide identification and analysis of DNA methyltransferase and demethylase gene families in Dendrobium officinale reveal their potential functions in polysaccharide accumulation

Genome-wide identification and analysis of DNA methyltransferase and demethylase gene families in Dendrobium officinale reveal their potential functions in polysaccharide accumulation

Phenolic characteristics acquired by berry skins of Vitis vinifera cv. Tempranillo in response to close‐to‐ambient solar ultraviolet radiation are mostly reflected in the resulting wines

Transcriptomic and Metabolomic Basis of Short- and Long-Term Post-Harvest UV-C Application in Regulating Grape Berry Quality Development

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