Changes in Gut Bacterial Translation Occur before Symptom Onset and Dysbiosis in Dextran Sodium Sulfate-Induced Murine Colitis

Taguer, Mariia; Darbinian, Emma; Wark, Kim L.; Ter-Cheam, A; Stephens, David A.; Maurice, Corinne F.

doi:10.1128/msystems.00507-21

Cited by 5 publications

(9 citation statements)

References 88 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our mouse experiments and those carried out in a previous study 17 , the administration of DSS-induced gut inflammation, leading to a drastic alteration of the gut environment and subsequent changes in gut microbiota composition. However, the chemically-induced gut inflamation did not reduce the total bacterial load in mouse feces.…”

Section: Discussionmentioning

confidence: 54%

“…This regimen is known to cause a significant depletion of gut-resident bacteria 16 . We included two additional groups of (a) five untreated mice and (b) five mice treated with DSS (dextran sulfate sodium)—a chemical used to induce epithelial damage without impacting the total bacterial load in feces 17 —for comparison. Throughout the experiment, we collected paired fecal and oral samples, which were subsequently analyzed by 16S amplicon sequencing to profile bacterial composition and by 16S quantitative PCR (qPCR) to estimate total bacterial abundance.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

A High Fraction of Oral Bacteria in the Feces Indicates Gut Microbiota Depletion with Implications for Human Health

Liao

Rolling

Djukovic

et al. 2022

Preprint

View full text Add to dashboard Cite

High relative abundance of oral bacteria in the gut is considered abnormal and has been linked to intestinal disorders. However, this reported association has two alternate explanations: ectopic oral bacteria may increase in absolute numbers in the intestine and drive disease (the driver hypothesis) or, instead, intestinal commensals may decrease in absolute numbers and the relative rise in oral bacteria is simply an indicator of this loss (the marker hypothesis). Here we use experiments in mice and clinical data from patients to provide evidence in favor of the marker hypothesis. We demonstrate that depletion of intestinal bacteria by antibiotics increases the relative abundance of oral bacteria in the host feces without increasing their absolute abundance, and the relative enrichment can indicate host health parameters such as stool consistency, pathogen overgrowth, and immunity. Our results reframe the conventional interpretation of microbiome sequencing data with implications for intestinal disorders and microbiome therapies.

show abstract

Section: Discussionmentioning

confidence: 54%

Section: Resultsmentioning

confidence: 99%

A High Fraction of Oral Bacteria in the Feces Indicates Gut Microbiota Depletion with Implications for Human Health

Liao

Rolling

Djukovic

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…Thus, the dynamic alterations of the gut microbiota during colitis progression remain less characterized. 19,20,29,[42][43][44][45][46][47][48][49][50] By monitoring murine fecal microbial communities in DSS colitis, we aimed to determine some of these underlying microbial dynamics of colitis. While we do not report significant decreases in alpha-diversity, unlike other DSS studies 48,[50][51][52] , our beta-diversity analyses suggest that these gut bacterial communities may have reached an alternative stable state distinct from baseline.…”

Section: Dss Colitis Results In Broad-level and Taxon-specific Change...mentioning

confidence: 99%

“…Reference source not found.). 28 In this way, we defined the different health states of the mice during colitis development: baseline, pre-symptomatic, symptomatic, and recovery, 29 as detailed in the Methods. Amplicon sequencing of the 16S V4-5 region was conducted on bacterial DNA extracted from mouse feces collected throughout the experiment, as described in the Methods.…”

Section: Dss Reproducibly Causes Colitis and Changes In Fecal Bacteri...mentioning

confidence: 99%

“…We incorporated these three parameters into a modified scoring metric of colitis as described by Kim et al (2012) based on the disease activity index (DAI) (Supplementary Table 1, Supplementary Table 2). 28 In this way, we defined the different health states of the mice during colitis development: baseline, presymptomatic, symptomatic, and recovery, 29 as detailed in the Methods. Amplicon sequencing of the 16S V4-5 region was conducted on bacterial DNA extracted from mouse feces collected throughout the experiment, as described in the Methods.…”

Section: Dss Reproducibly Causes Colitis and Changes In Fecal Bacteri...mentioning

confidence: 99%

See 1 more Smart Citation

Dextran sodium sulfate-induced colitis alters the proportion and composition of replicating gut bacteria

Beauchemin,

Hunter,

Maurice

2024

Preprint

Self Cite

View full text Add to dashboard Cite

The bacteria living in the human gut are essential for host health. Though the composition and metabolism of these bacteria is well described in both healthy hosts and those with intestinal disease, less is known about the activity of the gut bacteria prior to, and during, disease development, especially regarding gut bacterial replication. Here, we use a recently developed single-cell technique alongside existing metagenomics-based tools to identify, track, and quantify the replicating gut bacteria and their replication dynamics in the dextran sodium sulfate mouse model of colitis. We show that the proportion of replicating gut bacteria decreases when mice have the highest levels of inflammation and returns to baseline levels as mice begin recovering. We additionally report significant alterations in the composition of the total replicating gut bacterial community during colitis development. On the taxa level, we observe significant changes in the abundance of taxa such as the mucus-degrading Akkermansia muciniphila and the poorly described Erysipelatoclostridium genus. We further demonstrate that many taxa exhibit variable replication rates during colitis, including A. muciniphila. Lastly, we show that colitis development is positively correlated with increases in the presence and abundance of bacteria predicted to be fast replicators, suggesting that taxa with the potential to replicate quickly may have an advantage during intestinal inflammation. These data support the need for additional research using activity-based approaches to further characterize the gut bacterial response to intestinal inflammation and its consequences for both the host and the gut microbial community at large.

show abstract

Oral bacteria relative abundance in faeces increases due to gut microbiota depletion and is linked with patient outcomes

Liao,

Rolling,

Djukovic

et al. 2024

Nat Microbiol

View full text Add to dashboard Cite

Changes in Gut Bacterial Translation Occur before Symptom Onset and Dysbiosis in Dextran Sodium Sulfate-Induced Murine Colitis

Abstract: Most studies on the gut microbiome focus on the composition of this community and how it changes during disease. However, how the community transitions from a healthy state to one associated with disease is currently unknown.

Cited by 5 publications

References 88 publications

A High Fraction of Oral Bacteria in the Feces Indicates Gut Microbiota Depletion with Implications for Human Health

A High Fraction of Oral Bacteria in the Feces Indicates Gut Microbiota Depletion with Implications for Human Health

Dextran sodium sulfate-induced colitis alters the proportion and composition of replicating gut bacteria

Oral bacteria relative abundance in faeces increases due to gut microbiota depletion and is linked with patient outcomes

Contact Info

Product

Resources

About