Changes in haematopoietic progenitor colony differentiation and proliferation and the production of different abzymes in <scp>EAE</scp> mice treated with <scp>DNA</scp>

Aulova, Kseniya S.; Toporkova, Ludmila B.; Lopatnikova, Julia; Alshevskaya, Alina; Sennikov, Sergei V.; Buneva, Valentina N.; Budde, Thomas; Meuth, Sven G.; Попова, Н. А.; Orlovskaya, Irina A.; Nevinsky, Georgy A.

doi:10.1111/jcmm.13289

Cited by 27 publications

(350 citation statements)

References 37 publications

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“…There are several different EAE models, each mimicking a specific facet of MS (for review see). C57BL/6 mice were recently used to study possible mechanisms of spontaneous, myelin oligodendrocyte glycoprotein (MOG)‐ and DNA‐accelerated development of EAE . We confirmed that immunization with MOG and DNA led to an acceleration of EAE development but was associated with various changes in HSC differentiation profiles, lymphocyte proliferation and apoptosis in different organs of mice.…”

Section: Introductionsupporting

confidence: 56%

“…Immunization of mice with MOG and Pertussis toxin, and immunization with DNA without toxin was carried out as described in previously published protocol . The relative weight of mice and proteinuria (relative concentration of total protein in the urine, mg/mL) was analysed as before . Protein concentration in urine was measured using the Bradford assay with a bovine serum albumin standard.…”

Section: Methodsmentioning

confidence: 99%

“…Protein concentration in urine was measured using the Bradford assay with a bovine serum albumin standard. For further experiments including the purification of Abs and analysis of their enzymatic activity, 0.5‐0.8 mL of blood was collected after decapitation using standard approaches …”

Section: Methodsmentioning

confidence: 99%

“…DNase activity of IgGs was analysed according to published methods . The reaction mixture (20 μL) for analysis of IgG DNase activity contained 20 mmol/L Tris‐HCl (pH 7.5), 20 mmol/L NaCl, 5 mmol/L MgCl 2 , 1 mmol/L ethylenediaminetetraacetic (EDTA), 20 μg/mL supercoiled (sc) pBluescript and 0.03‐0.2 mg/mL of IgGs, and was incubated for 1‐12 hours at 37°C.…”

Section: Methodsmentioning

confidence: 99%

“…At that time, antibody titres for various anti‐antigens correspond to a range of indices typical for healthy humans and mice. A detectable level of abzymes can indicate the disease onset or pre‐disease stage, while an increase in catalytic activities follows the development of clear pathological symptoms . To understand the disease, it is therefore essential to identify all possible parallel and complementary mechanisms of MS development.…”

Section: Introductionmentioning

confidence: 99%

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Changes in cell differentiation and proliferation lead to production of abzymes in EAE mice treated with DNA–Histone complexes

Aulova

Toporkova

Lopatnikova

et al. 2018

J Cellular Molecular Medi

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Experimental autoimmune encephalomyelitis (EAE)‐prone C57BL/6 mice are used as a model of human multiple sclerosis. We immunize mice with myelin oligodendrocyte glycoprotein (MOG), DNA–histone and DNA‐methylated bovine serum albumin (met‐BSA) complexes to reveal different characteristics of EAE development including bone marrow lymphocyte proliferation and differentiation profiles of hematopoietic stem cells. Immunization of C57BL/6 mice with MOG35‐55 results in the acceleration of EAE development. Anti‐DNA antibodies are usually directed against DNA–histone complexes resulting from cell apoptosis. During the acute EAE phase (7‐20 days after immunization), catalytic antibodies efficiently hydrolysing myelin basic protein (MBP), MOG and DNA are produced with parallel suppression of antibodies hydrolysing histones. We could show that in contrast to MOG, immunization with histone‐DNA results in a reduction of proteinuria, a significant increase in anti‐DNA, anti‐MBP and anti‐MOG antibody titres, as well as an increase in their catalytic activities for antigen hydrolysis, but slightly changes the concentration of cytokines. Contrary to MOG, DNA–histone and DNA‐met‐BSA only stimulated the formation of anti‐DNA antibodies hydrolysing DNA with a long delay (15‐20 days after immunization). Our data indicate that for C57BL/6 mice immunization with DNA‐met‐BSA and DNA–histone complexes may have opposing effects compared to MOG. DNA–histone stimulates the appearance of histone‐hydrolysing abzymes in the acute EAE phase, while abzymes with DNase activity appear at significantly later time‐points. We conclude that MOG, DNA–histone and DNA‐met‐BSA have different effects on numerous bone marrow, cellular, immunological and biochemical parameters of immunized mice, but all antigens finally significantly stimulate the development of the EAE.

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Section: Introductionsupporting

confidence: 56%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Changes in cell differentiation and proliferation lead to production of abzymes in EAE mice treated with DNA–Histone complexes

Aulova

Toporkova

Lopatnikova

et al. 2018

J Cellular Molecular Medi

Self Cite

252

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Substrate specificity of IgGs with peroxidase and oxidoreductase activities from sera of patients with systemic lupus erythematosus and multiple sclerosis

Tolmacheva

Buneva

Nevinsky

2019

J of Molecular Recognition

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The analysis of IgGs to protect humans from oxidative stress through oxidation of harmful compounds was carried out. We have compared here for the first time peroxidase (in the presence of H 2 O 2 ) and oxidoreductase (in the absence of H 2 O 2 ) activities of IgGs from sera of healthy humans and patients with systemic lupus erythematosus (SLE) and multiple sclerosis (MS). In addition, substrate specificity of SLE and MS IgG preparations in the oxidation of different compounds was analyzed: 2,2′azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 3,3′-diaminobenzidine (DAB), homovanillic acid (HVA), o-phenylenediamine (OPD), α-naphthol, 3-amino-9ethylcarbazole (AEC), p-hydroquinone (pHQ), and adrenaline. IgGs of healthy humans and SLE and MS patients oxidized DAB, ABTS, and OPD due to their peroxidase and oxidoreductase activities, while other compounds were substrates of IgGs only in the presence of H 2 O 2 : adrenaline was not oxidized by both activities of IgGs. The average SLE IgGs peroxidase activity increased statistically significant in comparison with abzymes from healthy humans in the order (-fold): OPD (1.2) < DAB (1.7) < α-naphtol (2.2) ≤ AEC (2.4) < ABTS (4.5) < 5-ASA (10.6), while with oxidoreductase activity: OPD (1.8) ≤ DAB (2.1-fold) < ABTS (5.0). Only HVA was oxidized by IgGs with peroxidase activity of healthy donors faster than by SLE (1.3-fold) and MS abzymes (2.4-fold). In the oxidation of several substrates, only three IgGs of MS patients were used. The data speak of a tendency to increase the peroxidase and oxidoreductase activities of MS IgGs in comparison with healthy donors, but to a lesser extent: OPD (1.1 to 1.2-fold) ≤ ABTS (1.2 to 1.8-fold). It was shown that development of SLE and MS leads to increase in peroxidase and oxidoreductase activities of IgGs toward most of classical substrates. Thus, abzymes can serve as an additional factor of reactive oxygen species detoxification protecting of patients with SLE and MS from some harmful compounds somewhat better than healthy peoples.

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Catalytic antibodies in the bone marrow and other organs of Th mice during spontaneous development of experimental autoimmune encephalomyelitis associated with cell differentiation

et al. 2021

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Exact mechanisms of autoimmune disease development are still yet unknown. However, it is known that the development of autoimmune diseases is associated with defects in the immune system, namely, the violation of the bone marrow hematopoietic stem cells (HSCs) differentiation profiles. Different characteristics of autoimmune reaction development in experimental autoimmune encephalomyelitis (EAE) prone Th mice characterizing T-lymphocytes response were analyzed using standard approaches. Profiles of several HSCs differentiation of bone marrow (BFU-E, CFU-E, CFU-GM, CFU-GEMM, T- and B-lymphocytes) of Th male and female mice during spontaneous development of EAE were noticeably different. Patterns of total lymphocytes, B- and T-cells proliferation in several different organs (bone marrow, blood, spleen, thymus, and lymph nodes) were also remarkably different. In addition, there were in time noticeable differences in their changes for some organs of male and female mice. Characters of changes in the profiles of CD4 and CD8 cells proliferation in some organs not always coincide with those for total T lymphocytes. The changes in the differentiation profiles of HSCs and the level of lymphocytes proliferation in the bone marrow and other organs were associated with the increase in the concentration of antibodies against DNA, myelin basic protein, and myelin oligodendrocyte glycoprotein, and catalytic antibodies hydrolyzing these substrates. Despite some differences in changes in the analyzed parameters, in general, the spontaneous development of EAE in male and female mice occurs to some extent in a comparable way.

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Changes in haematopoietic progenitor colony differentiation and proliferation and the production of different abzymes in EAE mice treated with DNA

Cited by 27 publications

References 37 publications

Changes in cell differentiation and proliferation lead to production of abzymes in EAE mice treated with DNA–Histone complexes

Changes in cell differentiation and proliferation lead to production of abzymes in EAE mice treated with DNA–Histone complexes

Substrate specificity of IgGs with peroxidase and oxidoreductase activities from sera of patients with systemic lupus erythematosus and multiple sclerosis

Catalytic antibodies in the bone marrow and other organs of Th mice during spontaneous development of experimental autoimmune encephalomyelitis associated with cell differentiation

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