Changes in indole-3-acetic acid, indole-3-acetic acid oxidase, and peroxidase isoenzymes in the seeds of developing peach fruits

Valpuesta, Victoriano; Quesada, Miguel A.; Sánchez‐Roldán, Cristina; Tigier, Horacio A.; Heredia, Antonio; Bukovac, Martin J.

doi:10.1007/bf02021818

Cited by 14 publications

(17 citation statements)

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“…Thus, the idea proposed by Galston and Dalberg in 1954 that increased IAA-oxidase might account for the decreased IAA level in older, non-growing tissues has long been accepted. However, several studies have reported higher 1AA-oxidase in younger growing tissues which also exhibited higher IAA concentrations (Rajagopal and Larsen 1974;Brennan and Jacobs 1983;Valpuesta et al 1989;Sfinchez-Bravo et al 1989). Though a compartmentalization of IAA and IAA-oxidase might explain these results, the presence of a high IAA concentration in tissues with a high capacity to destroy IAA would agree with the proposed hypothesis that IAA must be destroyed following "the growth promoting act" (Cohen and Bandurski 1978).…”

Section: Introductionsupporting

confidence: 74%

Uptake and decarboxylation of indole-3-acetic acid during auxin-induced growth in lupin hypocotyl segments

Botía

Ortuño

Sabater

et al. 1994

Planta

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The elongation growth of etiolated hypocotyl segments of lupin (Lupinus albus L.) was stimulated by acid pH (4.6 versus 6.5) and by IAA for periods of up to 4 h. After this time, the segments were unable to grow further. In the presence of an optimal IAA concentration (10 gM), acid pH increased the growth rate but had no effect on final growth. With suboptimal IAA (0.1 laM), however, acid pH increased growth in a more than additive way, suggesting a synergistic action between the two factors. This synergism may be explained by the increased IAA uptake and decarboxylation seen at an acid pH. These results reinforce the view that the effects of low pH and IAA on growth are not independent. Vanadate inhibited growth and also IAA uptake and decarboxylation. This inhibitor, therefore, probably inhibits growth not only by decreasing ATPase-mediated acidification but also by decreasing H+-dependent IAA uptake from the apoplasm. This dependence of IAA uptake on ATPase may be mediated by apoplasmic acidification. The amount of IAA decarboxylated increased when the assay conditions favored the growth of segments, indicating that IAA could be destroyed by decarboxylation during the auxin-induced growth.

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Section: Introductionsupporting

confidence: 74%

Uptake and decarboxylation of indole-3-acetic acid during auxin-induced growth in lupin hypocotyl segments

Botía

Ortuño

Sabater

et al. 1994

Planta

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“…A persistência e o crescimento do fruto na planta dependem das relações exatas entre auxinas, giberelinas e citocininas (POWELL; PRATTI, 1966, VALPUESTA et al, 1989. Esses hormônios condicionam o desenvolvimento dos frutos caracterizado por uma curva sigmoidal dupla, com três estádios distintos (BARBOSA et al, 1993;CUNHA JÚNIOR et al, 2007;DELA BRUNA, 2007).…”

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Desenvolvimento dos frutos de pêssego 'Aurora' e nectarina 'Sunraycer' no sul de Santa Catarina

Bruna¹,

Moreto²

2011

Rev. Bras. Frutic.

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RESUMO -O desenvolvimento do fruto de pessegueiro é resultado da diferenciação e do crescimento das paredes do ovário após a fecundação. A persistência e o crescimento do fruto na planta dependem das relações exatas entre os hormônios auxina, giberelina e citocinina que condicionam o desenvolvimento dos frutos, caracterizado por uma curva dupla sigmoide, com três estádios distintos. O presente trabalho teve por objetivo conhecer o comportamento dos frutos e das sementes do pêssego Aurora e da Nectarina Sunraycer durante todo o seu ciclo de desenvolvimento. O crescimento dos frutos e das sementes durante o ciclo foi determinado semanalmente, coletando-se 30 frutos de dez diferentes plantas em ramos previamente identificados. As sementes foram separadas do fruto para a determinação do peso fresco (PF) e do peso seco (PS). O crescimento dos frutos da variedade Sunraycer dá-se de forma contínua e acelerada desde a floração até a maturação, sugerindo um curto período ou a inexistência do Estágio II de crescimento. Para a variedade Aurora, a curva de crescimento é diferenciada nos três estádios (I, II e III). O raleio dos frutos deve ser feito até o início do estádio II, para a variedade Aurora e Sunraycer. As sementes das variedades Aurora e Sunraycer atingem seu tamanho máximo no estágio I de crescimento do fruto. O aumento de peso seco na semente, para a variedade Sunraycer, é praticamente inexistente no estádio III, enquanto para a variedade Aurora ocorre o maior aumento de peso seco que vai até a maturação do fruto. Termos para indexação: Curva de crescimento, Produção, Ecofisiologia. DEVELOPMENT OF THE 'AURORA' PEACH AND 'SUNRAYCER' NECTARINE FRUITS IN SOUTHERN SANTA CATARINA, BRAZILABSTRACT -The peach fruit development is a result of the differentiation and growth of the ovary wall after fertilization. The persistency and the growth of the fruit on the plant depend on the exact relationships among auxin, gibberelin and cytokinin hormones, which condition the development of the fruits, characterized by a double sigmoid curve with three distinct stages. This study aimed to understand the performance of fruits and seeds of the Aurora peach and Sunraycer nectarine throughout their development cycle. Fruits and seeds growth was evaluated weekly by collecting 30 fruits from previously identified branches of ten different plants. The seeds were separated from the fruit for the determination of the fresh weight (FW) and the dry weight (DW). The growth of the Sunraycer fruits variety is continuous and accelerated from flowering to maturity, which suggests a short period or absence of the growth Stage II. For the Aurora variety, the growth curve is differentiated during the three stages (I, II and III). The fruits thinning must be done until the beginning of stage II for both varieties, Aurora and Sunraycer. Their seeds reach their maximum size at stage I of fruit growth. The increase in the dry weight of the Sunraycer variety was practically absent on stage III, whereas it increased significantly in the Aurora variety u...

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“…The seed apparently plays a critical role in growth and viability of the fruit during stage I (Tukey 1936). Changes in indoleacetic acid (IAA) levels and peroxidase activities have been determined previously along with the fruit growth (Valpuesta et al 1989).The main soluble anionic peroxidase isoenzyme from peach seeds was purified, and rabbit polyclonal antibodies were obtained against this isoenzyme (Quesada et al 1990). Changes in total IAA oxidase activity of peach complete seed peroxidases and levels of specific peroxidase proteins, detected by polyclonal antibodies, were followed during fruit growth (Alba et al 1993).…”

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confidence: 99%

mentioning

confidence: 99%

Localization and General Properties of Developing Peach Seed Coat and Endosperm Peroxidase Isoenzymes

Alba¹,

Forchetti²,

Quesada

et al. 1998

J Plant Growth Regul

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Changes of soluble and ionically bound peroxidase and indoleacetic acid (IAA) oxidase activities were followed during peach seed development. Soluble peroxidase activity was located mainly in the embryo plus endosperm tissue, whereas wall ionically bound activities were found predominantly in the integument tissue. The different peroxidase isoenzymes present in the extracts were characterized by polyacrylamide gel electrophoresis and isoelectric focusing; the main soluble isoenzyme of embryo plus endosperm tissue was an anionic isoperoxidase of R F 0.07. Basic ionically bound isoenzymes were located only in the integument tissue, but two soluble anionic isoenzymes of R F 0.23 and 0.51 were also present in this tissue. In parallel, peroxidase protein content was estimated specifically using polyclonal antibodies. The kinetic data and the changes of seed IAA oxidase activity during fruit development suggested that basic peroxidase isoenzymes from ionically bound extracts of integument might be involved in IAA degradation.The peach fruit is a useful model for studying growthrelated changes in peroxidase isoenzyme patterns and their relationships with some physiological processes because its growth occurs in three well defined development stages (Valpuesta et al. 1991). The seed apparently plays a critical role in growth and viability of the fruit during stage I (Tukey 1936). Changes in indoleacetic acid (IAA) levels and peroxidase activities have been determined previously along with the fruit growth (Valpuesta et al. 1989).The main soluble anionic peroxidase isoenzyme from peach seeds was purified, and rabbit polyclonal antibodies were obtained against this isoenzyme (Quesada et al. 1990). Changes in total IAA oxidase activity of peach complete seed peroxidases and levels of specific peroxidase proteins, detected by polyclonal antibodies, were followed during fruit growth (Alba et al. 1993).Here we report the first differential distribution of peroxidase isoenzymes and changes in the levels of specific protein in each seed tissue, integument (the outer coat of seed) and embryo plus endosperm, during fruit development. The kinetic behavior of IAA oxidase activity of the isoenzymes with the highest activity with this substrate was analyzed in order to study the possible role of peroxidases during peach seed development. Materials and Methods PlantsFruits were obtained from a commercial cultivar (cv. Redhaven) of peach (Prunus persica L. Batsch) during fruit growth. Several samples were collected during the three stages of fruit development defined previously for the cultivar Redhaven (Alba et al. 1995). Stage I extends from 0 to 55 days, stage II from 55 to 79 days, and stage III from 79 to 98 days after anthesis. Seeds were removed from frozen fruit and dissected mechanically into integument and embryo plus endosperm as a unique section. Crude Extract Isolation ProcedureSoluble protein extracts and those ionically bound to cell walls were obtained by grinding the seeds and their different sections with 50 mM sodium ...

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Changes in indole-3-acetic acid, indole-3-acetic acid oxidase, and peroxidase isoenzymes in the seeds of developing peach fruits

Cited by 14 publications

References 14 publications

Uptake and decarboxylation of indole-3-acetic acid during auxin-induced growth in lupin hypocotyl segments

Uptake and decarboxylation of indole-3-acetic acid during auxin-induced growth in lupin hypocotyl segments

Desenvolvimento dos frutos de pêssego 'Aurora' e nectarina 'Sunraycer' no sul de Santa Catarina

Localization and General Properties of Developing Peach Seed Coat and Endosperm Peroxidase Isoenzymes

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