Changes in ingestive behavior, serum glucose and free fatty acids concentrations in rats following intracerebroventricular injection of spermine.

Kohno, Hiroyuki; Sakurada, Tsukasa; Suzuki, Tomoharu; Kisara, Kensuke; Satoh, Hiroaki

doi:10.1254/jjp.31.863

Cited by 11 publications

(2 citation statements)

References 31 publications

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“…The increased food intake may underlie changes specifically within the PVN, which produces anorexigenic peptides such as thyrotropin-releasing hormone, CRH, and OT ( 58 ), or simply may reflect the increased hydration status of Azin1 shRNA animals, as indicated by the lower plasma osmolality. Interestingly, spermine has been shown to suppress food intake when administered intracerebroventricularly into the rat brain, in agreement with a role of polyamines on ingestive behavior ( 59 ).…”

Section: Discussionsupporting

confidence: 56%

Control of Polyamine Biosynthesis by Antizyme Inhibitor 1 Is Important for Transcriptional Regulation of Arginine Vasopressin in the Male Rat Hypothalamus

Greenwood

Paton

et al. 2015

Endocrinology

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The polyamines spermidine and spermine are small cations present in all living cells. In the brain, these cations are particularly abundant in the neurons of the paraventricular (PVN) and supraoptic nuclei (SON) of the hypothalamus, which synthesize the neuropeptide hormones arginine vasopressin (AVP) and oxytocin. We recently reported increased mRNA expression of antizyme inhibitor 1 (Azin1), an important regulator of polyamine synthesis, in rat SON and PVN as a consequence of 3 days of dehydration. Here we show that AZIN1 protein is highly expressed in both AVP- and oxytocin-positive magnocellular neurons of the SON and PVN together with antizyme 1 (AZ1), ornithine decarboxylase, and polyamines. Azin1 mRNA expression increased in the SON and PVN as a consequence of dehydration, salt loading, and acute hypertonic stress. In organotypic hypothalamic cultures, addition of the irreversible ornithine decarboxylase inhibitor DL-2-(difluoromethyl)-ornithine hydrochloride significantly increased the abundance of heteronuclear AVP but not heteronuclear oxytocin. To identify the function of Azin1 in vivo, lentiviral vectors that either overexpress or knock down Azin1 were stereotaxically delivered into the SON and/or PVN. Azin1 short hairpin RNA delivery resulted in decreased plasma osmolality and had a significant effect on food intake. The expression of AVP mRNA was also significantly increased in the SON by Azin1 short hairpin RNA. In contrast, Azin1 overexpression in the SON decreased AVP mRNA expression. We have therefore identified AZIN1, and hence by inference, polyamines as novel regulators of the expression of the AVP gene.

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Section: Discussionsupporting

confidence: 56%

Control of Polyamine Biosynthesis by Antizyme Inhibitor 1 Is Important for Transcriptional Regulation of Arginine Vasopressin in the Male Rat Hypothalamus

Greenwood

Paton

et al. 2015

Endocrinology

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“…0.6 mm in diameter) with an inner obdulator (approx. 0.3 mm in diameter) was implanted stereotaxically into the left lateral, 3rd and 4th cerebroventricles (14) according to the atlas of Pellegrino et al (15).…”

Section: Animalsmentioning

confidence: 99%

Comparison of the antinociceptive effect between the cyclic dipeptide cyclo (Tyr(Et)-homoarginine) and the linear dipeptide Boc-Tyr(Et)-homoarginine-OMe in rats.

Sato¹,

Sakurada²,

Sakurada³

et al. 1984

Jpn.J.Pharmacol

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Abstract-The antinociceptive effect of the cyclic dipeptide cyclo[Tyr(Et)-homo arginine] (C.TEHA) was examined utilizing the tail flick test and the digitus pinching test in rats in comparison with the linear dipeptide Boc-Tyr(Et)-homoarginine OMe (B.TEHM).Though both dipeptides administered into the lateral, 3rd and 4th cerebroventricles produced antinociceptive effects equipotent to morphine, except for the 4th cerebroventricular administration of B.TEH M, the administration into the spinal subarachnoid space was without effect. The effect of B.TEHM was com pletely antagonized by the pretreatment of naloxone (i.p.) when administered into -ihe 3rd cerebroventricle where its effect was demonstrated to be the most potent .

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The significance of?-adrenoceptor down regulation in the desipramine action in the forced swimming test

Kitada

Miyauchi

Kosasa

et al. 1986

Naunyn-Schmiedeberg's Arch. Pharmacol.

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The present studies were undertaken to clarify whether central beta-adrenoceptor down regulation is responsible for the greater effect of chronic treatment with desipramine (DMI) compared with acute treatment in the forced swimming test in rats. Repetitive administration of DMI activated the rat behaviour pattern and consequently reduced the duration of immobility. The degree of activation depended on the length of treatment, i.e. no effect when given in a single dose, moderate effect when given subchronically (3 doses) and marked activation after chronic (31 doses) treatment. Chronic treatment with DMI also produced a decrease in 3H-dihydroalprenolol (3H-DHA) binding site in the cerebral cortex. Acute stimulation of brain beta-adrenoceptors by intracerebroventricular (i.c.v.) isoprenaline significantly, though partially, attenuated the behavioural effect of chronic DMI by beta 1-adrenoceptor-related mechanisms. Similarly, chronic i.c.v. co-administration of atenolol or practolol, beta 1-adrenoceptor antagonists, together with DMI attenuated both beta-adrenoceptor down regulation and the behavioural activation by chronic DMI. On the other hand, chronic i.c.v. administration of isoprenaline, supposedly leading to down regulation of beta-adrenoceptors, facilitated the activating behavioural effect of DMI, as a single dose became effective. Changes, however, in 3H-DHA binding parameters in the cerebral cortex were not observed after chronic isoprenaline. These results suggest that down regulation of beta-adrenoceptors in brain is responsible, at least in part, for the marked activatory effect of chronic DMI in the forced swimming test, possibly by reducing an inhibitory function of beta 1-adrenoceptor mediated mechanisms.

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Changes in ingestive behavior, serum glucose and free fatty acids concentrations in rats following intracerebroventricular injection of spermine.

Cited by 11 publications

References 31 publications

Control of Polyamine Biosynthesis by Antizyme Inhibitor 1 Is Important for Transcriptional Regulation of Arginine Vasopressin in the Male Rat Hypothalamus

Control of Polyamine Biosynthesis by Antizyme Inhibitor 1 Is Important for Transcriptional Regulation of Arginine Vasopressin in the Male Rat Hypothalamus

Comparison of the antinociceptive effect between the cyclic dipeptide cyclo (Tyr(Et)-homoarginine) and the linear dipeptide Boc-Tyr(Et)-homoarginine-OMe in rats.

The significance of?-adrenoceptor down regulation in the desipramine action in the forced swimming test

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