Soils are a source of atmospheric nitrogen oxides (NO x), especially in regions with significant cropland where nitrogen (N) fertilizers are used to enhance crop yields. The magnitude of soil NO x emissions, however, varies substantially by region, depending on the local land use pattern and management activities. We estimated soil NO x emissions in California based on the DeNitrification-DeComposition (DNDC) biogeochemical model, linked to a detailed spatial-temporal differentiated California-specific database. The DNDC-generated surface fluxes were used in the Community Multiscale Air Quality (CMAQ) model to evaluate impacts of soil NO x emissions on formation of ambient particulate (PM 2.5) nitrate in the San Joaquin Valley (SJV) where cropland is the dominant land use. The DNDC-generated soil NO x emissions contribute approximately 1.1% of total anthropogenic NO x emissions in California, at an emission rate of roughly 24 t day −1 (as NO 2) statewide and 9 t day −1 in the SJV. Cropland is the dominant source of soil NO x emissions in California, contributing nearly 60% of statewide soil NO x emissions, driven principally by fertilizer use. The PM 2.5 nitrate concentrations simulated by CMAQ using the DNDC-generated soil NO x emissions are compatible with those observed in the SJV, suggesting that soil NO x emissions have limited impacts on PM 2.5 nitrate formation in the atmosphere. Our emission and air quality modeling results are further supported by long-term ambient NO x-to-carbon monoxide (CO) and satellite NO 2 data analyses in the SJV, which showed diurnal, monthly, and annual trends consistent with characteristics of NO x sources dominated by traffic combustion in both urban and agricultural regions. Plain Language Summary Nitrogen oxides (NO x) are air pollutants that can react with other chemicals in the air to form fine particulate matter and ozone, both of which pose adverse impacts to human health and the environment. Control of NO x emissions is critical to improving air quality in California. Soils are a known NO x source, especially in agricultural areas where large amounts of nitrogen fertilizers are used to increase crop yields. The nitrogen chemicals in soil can be converted into various nitrogen gases, including NO x , by soil microorganisms. The contribution of soil emissions to the total NO x budget varies by region, depending on land uses and management activities. This study modeled soil NO x emissions from different land covers in California and evaluated impacts of soil NO x emissions on the formation of ambient particulate nitrate in the San Joaquin Valley (SJV), where cropland is the dominant land use. Our results indicate that soil NO x is a relatively minor fraction of the total NO x budget in California and has a minor effect on atmospheric concentrations of particulate nitrate in the SJV. Ambient and satellite data analyses show traffic combustions being the dominate source of NO x emissions in both urban and agricultural areas of the SJV.